LOCUS       sequence-382075-        1980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-based purification of 
            Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal 
            mNeonGreen tag and cleavable N-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Chen F, Wu J, Iwanski MK, Jurriens D, Sandron A, Pasolli M, Puma G, 
            Kromhout JZ, Yang C, Nijenhuis W, Kapitein LC, Berger F, Akhmanova A
  TITLE     Self-assembly of pericentriolar material in interphase cells lacking
            centrioles.
  JOURNAL   Elife. 2022 Jul 5;11:e77892. doi: 10.7554/eLife.77892.
  PUBMED    35787744
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-07-5"; volume: "11:e77892. doi"; pages: " 
            10.7554/eLife.77892"
COMMENT     Sequence Label: pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen
FEATURES             Location/Qualifiers
     source          1..1980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1270..1980
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      1525..1534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgagcgcag aacgagaaat tccagccgag gacagcatca aagtggtctg ccgattccga
       61 ccgctgaacg acagcgaaga gaaggccggc tccaagttcg tggtcaagtt ccccaacaat
      121 gtggaggaga actgcatatc catagcgggc aaggtgtatt tgttcgacaa ggtcttcaaa
      181 ccgaatgcat cccaggaaaa ggtctacaat gaggcggcca agtccattgt tacggatgtc
      241 ctggccgggt acaatggaac gatattcgca tacggtcaga cgtcctccgg aaaaacgcat
      301 acgatggagg gcgtgatcgg ggactccgta aaacagggta tcatacctag gatcgtcaac
      361 gacattttca atcacatcta cgcgatggag gtgaacctgg agtttcacat caaggtctcc
      421 tactacgaga tctacatgga caagattcga gatctgttgg acgtctccaa ggtgaacctt
      481 agtgtgcacg aggataagaa ccgggtgccg tacgtcaagg gcgctacgga acggttcgtc
      541 tcgtcgccgg aggatgtttt cgaggtgatc gaggagggca aatccaatcg tcacatcgct
      601 gtgacaaaca tgaacgagca ttcttcgcga tcccactcag tattccttat caatgtgaag
      661 caggagaacc tggagaacca gaagaaacta tccggcaaac tctacctggt ggatttggcc
      721 ggatccgaga aggtttccaa gactggagcg gagggaaccg ttcttgatga agccaagaac
      781 atcaacaagt cgctgtcggc cttgggcaac gtaatttctg ccctggcgga cggaaacaaa
      841 acgcacatcc cctaccgtga ttccaagcta acgcgcatcc tgcaggagtc gctgggaggc
      901 aacgcacgca caaccatcgt catctgctgc tctccagcca gtttcaacga atctgaaacg
      961 aagtcaacgc tggacttcgg tcgtagagcc aagacagtga agaacgtggt ctgcgttaac
     1021 gaggagctta ctgccgagga atggaagcga cgctatgaaa aggagaagga aaagaacgcc
     1081 cgactaaagg gtaaggtgga gaagcttgag atcgagcttg cgcgctggag agcgggtgaa
     1141 actgttaagg cggaggagca aatcaacatg gaggatctca tggaggcaag cacgcccaac
     1201 ctggaagtgg aggcagcaca gacggcggcg gccgaggccg ctttggccgc ccagcgaacg
     1261 gctgaattca tggtgagcaa gggcgaggag gataacatgg cctctctccc agcgacacat
     1321 gagttacaca tctttggctc catcaacggt gtggactttg acatggtggg tcagggcacc
     1381 ggcaatccaa atgatggtta tgaggagtta aacctgaagt ccaccaaggg tgacctccag
     1441 ttctccccct ggattctggt ccctcatatc gggtatggct tccatcagta cctgccctac
     1501 cctgacggga tgtcgccttt ccaggccgcc atggtagatg gctccggata ccaagtccat
     1561 cgcacaatgc agtttgaaga tggtgcctcc cttactgtta actaccgcta cacctacgag
     1621 ggaagccaca tcaaaggaga ggcccaggtg aaggggactg gtttccctgc tgacggtcct
     1681 gtgatgacca actcgctgac cgctgcggac tggtgcaggt cgaagaagac ttaccccaac
     1741 gacaaaacca tcatcagtac ctttaagtgg agttacacca ctggaaatgg caagcgctac
     1801 cggagcactg cgcggaccac ctacaccttt gccaagccaa tggcggctaa ctatctgaag
     1861 aaccagccga tgtacgtgtt ccgtaagacg gagctcaagc actccaagac cgagctcaac
     1921 ttcaaggagt ggcaaaaggc ctttaccgat gtgatgggca tggacgagct gtacaagtga
//
