LOCUS       sequence-382095-        6210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6210)
  TITLE     GeneDetect Michael J Fox Foundation Shared Tools Plasmids 2022
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAM/SAR-CAG-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..6210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     promoter        1287..1564
                     /label=chicken beta-actin promoter
     primer_bind     1843..1865
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1927..1946
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    2033..2621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2086..2106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2504..2515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2648..2665)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2654..2878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3088..3417)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3102..3237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3402..3422
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3663..4251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3743..3762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4422..5213,5214..5282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5045..5064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5283..5387)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5455..5473
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5511..5533)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5633..5652
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5866..5885
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        6175..6193
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6175..6192
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggtacg atccccagct tgcatgcctg caggtcactg ttctcatcac atcatatcaa
      241 ggttatatac catcaatatt gccacagatg ttacttagcc ttttaatatt tctctaattt
      301 agtgtatatg caatgatagt tctctgattt ctgagattga gtttctcatg tgtaatgatt
      361 atttagagtt tctctttcat ctgttcaaat ttttgtctag ttttattttt tactgatttg
      421 taagacttct ttttataatc tgcatattac aattctcttt actggggtgt tgcaaatatt
      481 ttctgtcatt ctatggcctg acttttctta atggtttttt aattttaaaa ataagtctta
      541 atattcatgc aatctaatta acaatctttt ctttgtggtt aggactttga gtcataagaa
      601 atttttctct acactgaagt catgatggca tgcttctata ttattttcta aaagatttaa
      661 agttttgcct tctccattta gacttataat tcactggaat ttttttgtgt gtatggtatg
      721 acatatgggt tcccttttat tttttacata taaatatatt tccctgtttt tctaaaaaag
      781 aaaaagatca tcattttccc attgtaaaat gccatatttt tttcataggt cacttacata
      841 tatcaatggg tctgtttctg agctctactc tattttatca gcctcactgt ctatccccac
      901 acatctcatg ctttgctcta aatcttgata tttagtggaa cattctttcc cattttgttc
      961 tacaagaata tttttgttat tgtcttttgg gcttctatat acattttaga atgaggttgg
     1021 caagttgggg atcgtaccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1081 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1141 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1201 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1261 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     1321 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     1381 agcgatgggg gcgggggggg ggggggggcg cgcgccaggs ggggsggggs ggggsgaggg
     1441 gsggggsggg gsgaggsgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     1501 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     1561 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     1621 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     1681 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     1741 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggctgtc
     1801 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1861 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1921 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttggatccac
     1981 tcgagtggag ctcgcgacta gtcgattcga attcgatatc aagcttatcg ataatcaacc
     2041 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2101 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2161 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2221 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2281 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2341 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2401 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     2461 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     2521 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     2581 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgactcgc
     2641 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2701 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2761 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2821 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2881 tctgaggcgg aaagaaccag ctggggctcg actagagcat ggctacgtag ataagtagca
     2941 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     3001 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggcggcct
     3061 cagtgagcga gcgagcgcgc agagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     3121 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     3181 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     3241 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     3301 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     3361 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     3421 ccacagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4081 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5401 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     5461 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5521 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5581 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     5641 gagcagattg tactgagagt gcaccattcg acgctctccc ttatgcgact cctgcattag
     5701 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
     5761 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     5821 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
     5881 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     5941 gaggatctgg ctagcgatga ccctgctgat tggttcgctg accatttccg ggtgcgggac
     6001 ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg actctgacgg cagtttacga
     6061 gagagatgat agggtctgct tcagtaagcc agatgctaca caattaggct tgtacatatt
     6121 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
     6181 gtgacactat agaatacacg gaattaattc
//
