LOCUS       sequence-38210-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinADE K108A HDEL
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(34..281)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     247..265
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     283..299
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     284..300
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    333..357
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa
       61 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
      121 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
      181 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
      241 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc gacggtatcg
      301 ataagcttga tatcgaattc ctgcagccca tcaccacttt gtacaagaaa gctgggtttc
      361 acaattcatc atgatcatcg tagtaataat ctaacttggt gaacaccgtt ttgcagcctt
      421 tatctgagaa aactctctcc tctttgggga aaaatgtcat ctcagccact ctgcttacaa
      481 tgtcttcatc aatttcatag cctcgggact gcatttccac tcggatacac atttttaggt
      541 gtttgtattc cagggggagg aagggaacaa aataatcaat gaggttccgg tcaattaagc
      601 tgctgtgcca gaagccactg ttcttgttat tgaaaaccga cacagacaac gcgtgttcaa
      661 tgtctttgag cttgatgtct tccctctgct ttccactcct ccagaaatcc aaagccacat
      721 ctgtgatcct ttctgctcca gcattgctga gaaatatgaa catggctttc tggtaggaga
      781 ccccatccac caggtcataa tagtcgagga aaggcttgat ggcatc
//
