LOCUS       sequence-382113-        9538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human MYCN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9538)
  TITLE     Roussel laboratory unpublished
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCDH-CMV-human MYCN-EF1-mRFP
FEATURES             Location/Qualifiers
     source          1..9538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2393..2604
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2617..2885
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2913..2922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3606..4193
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3658..3678)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4076..4087)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4267..4500
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4572..4693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4609..4628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4663..4682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4733..4868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4901..4917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4901..4917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4914..4936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4925..4941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4949..4955,4956..4973,4974..4979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4994..5015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5132..5149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5303..5891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6062..6853,6854..6922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6685..6704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6923..7027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7095..7113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7151..7173)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7273..7292
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7486..7508
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7500..7517
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7501..7517
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7532..7758
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7759..7939
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7986..8111
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8604..8837
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9022..9066
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9333..9449
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagattc tagagctagc gaattcggca cgaggctccg
      301 gtgtgtctgt cggttgcagt gttggaggtc ggcgccggcc cccgccttcc gcgcccccca
      361 cgggaaggaa gcacccccgg tattaaaacg aacggggcgg aaagaagccc tcagtcgccg
      421 gccgggaggc gagccgatgc cgagctgctc cacgtccacc atgccgggca tgatctgcaa
      481 gaacccagac ctcgagtttg actcgctaca gccctgcttc tacccggacg aagatgactt
      541 ctacttcggc ggccccgact cgaccccccc gggggaggac atctggaaga agtttgagct
      601 gctgcccacg cccccgctgt cgcccagccg tggcttcgcg gagcacagct ccgagccccc
      661 gagctgggtc acggagatgc tgcttgagaa cgagctgtgg ggcagcccgg ccgaggagga
      721 cgcgttcggc ctggggggac tgggtggcct cacccccaac ccggtcatcc tccaggactg
      781 catgtggagc ggcttctccg cccgcgagaa gctggagcgc gccgtgagcg agaagctgca
      841 gcacggccgc gggccgccaa ccgccggttc caccgcccag tccccgggag ccggcgccgc
      901 cagccctgcg ggtcgcgggc acggcggggc tgcgggagcc ggccgcgccg gggccgccct
      961 gcccgccgag ctcgcccacc cggccgccga gtgcgtggat cccgccgtgg tcttcccctt
     1021 tcccgtgaac aagcgcgagc cagcgcccgt gcccgcagcc ccggccagtg ccccggcggc
     1081 gggccctgcg gtcgcctcgg gggcgggtat tgccgcccca gccggggccc cgggggtcgc
     1141 ccctccgcgc ccaggcggcc gccagaccag cggcggcgac cacaaggccc tcagtacctc
     1201 cggagaggac accctgagcg attcagatga tgaagatgat gaagaggaag atgaagagga
     1261 agaaatcgac gtggtcactg tggagaagcg gcgttcctcc tccaacacca aggctgtcac
     1321 cacattcacc atcactgtgc gtcccaagaa cgcagccctg ggtcccggga gggctcagtc
     1381 cagcgagctg atcctcaaac gatgccttcc catccaccag cagcacaact atgccgcccc
     1441 ctctccctac gtggagagtg aggatgcacc cccacagaag aagataaaga gcgaggcgtc
     1501 cccacgtccg ctcaagagtg tcatcccccc aaaggctaag agcttgagcc cccgaaactc
     1561 tgactcggag gacagtgagc gtcgcagaaa ccacaacatc ctggagcgcc agcgccgcaa
     1621 cgaccttcgg tccagctttc tcacgctcag ggaccacgtg ccggagttgg taaagaatga
     1681 gaaggccgcc aaggtggtca ttttgaaaaa ggccactgag tatgtccact ccctccaggc
     1741 cgaggagcac cagcttttgc tggaaaagga aaaattgcag gcaagacagc agcagttgct
     1801 aaagaaaatt gaacacgctc ggacttgcta gacgcttctc aaaactggac agtcactgcc
     1861 actttgcaca ttttgatttt ttttttaaac aaacattgtg ttgacattaa gaatgttggt
     1921 ttactttcaa atcggtcccc tgtcgagttc ggctctgggt gggcagtagg accaccagtg
     1981 tggggttctg ctgggacctt ggagagcctg catcccagga tgctgggtgg ccctgcagcc
     2041 tcctccacct cacctccatg acagcgctaa acgttggtga cggttgggag cctctggggc
     2101 tgttgaagtc accttgtgtg ttccaagttt ccaaacaaca gaaagtcatt ccttcttttt
     2161 aaaatggtgc ttaagttcca gcagatgcca cataaggggt ttgccatttg atacccctgg
     2221 ggaacatttc tgtaaatacc attgacacat ccgccttttg tatacatcct gggtaatgag
     2281 aggtggcttt tgcggccagt attagactgg aagttcatac ctaagtcctg aattcgaatt
     2341 taaatcggat ccgcggccgc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     2401 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     2461 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2521 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2581 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     2641 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     2701 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     2761 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     2821 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     2881 agatccaagc tgtgaccggc gcctacgata tcgccaccat ggccagctcc gaggatgtca
     2941 tcaaagagtt tatgagattt aaggtcaaga tggagggaag cgtcaacgga cacgagttcg
     3001 agattgaggg agaaggagaa ggccggcctt acgagggcac acaaaccgct aagctcaagg
     3061 tcacaaaagg aggacccctc cccttctcct gggatattct gagccctcag ttccagtacg
     3121 gaagcaaagc ctatgttaaa caccctgccg acatccctga ctatctgaag ctctccttcc
     3181 ctgaaggctt caagtgggag agattcatga acttcgagga cggaggcgtg gtgacagtca
     3241 cacaagatag caccctccag gacggagagt ttatttataa ggtgaaactc agaggaacca
     3301 acttcccctc cgatggccct gtcatgcaaa aaaaaacaat gggatgggaa gcctccaccg
     3361 agagaatgta tcctgaggat ggcgctctga aaggcgaaat taaaatgaga ctgaaactca
     3421 aagacggagg acactacgat gccgaggtca aaacaaccta caaggccaag aaacaagtgc
     3481 agctgcctgg cgcctacatg actgatatta aactcgacat tatcagccat aatggggact
     3541 acaccatcgt ggaacaatat gagagagctg agggcagaca tagcacaggc gctggataag
     3601 tcgacaatca acctctgatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3661 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3721 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3781 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3841 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3901 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3961 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4021 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4081 cctcaatcca gcggaccttc cttcccgcgc cctgctgccg gctctgcggc ctcttccgcg
     4141 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac
     4201 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4261 ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg
     4321 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4381 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4441 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4501 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4561 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4621 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4681 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     4741 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4801 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4861 tggaggccta gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt
     4921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5701 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7021 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     7081 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     7141 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     7201 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     7261 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     7321 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     7381 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     7441 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     7501 gtaaaacgac ggccagtgcc aagctgacgc gtgtagtctt atgcaatact cttgtagtct
     7561 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     7621 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     7681 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7741 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7801 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7861 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7921 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7981 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     8041 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8101 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8161 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8221 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8281 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8341 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8401 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccactgatc
     8461 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     8521 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     8581 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8641 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8701 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8761 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8821 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8881 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8941 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     9001 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     9061 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     9121 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9181 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     9241 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     9301 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
     9361 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     9421 caaaaacaaa ttacaaaatt caaaatttta tcgatactag tattatgccc agtacatgac
     9481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
