LOCUS       sequence-38212-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinA E171Q HDEL
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(31..278)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     244..262
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     280..296
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     281..297
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    330..354
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actcactata gggcgaattg ggtaccggcc gcaaattaaa gccttcgagc gtcccaaaac
       61 cttctcaagc aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa
      121 aagaaaaatt tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat
      181 agggacctag acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg
      241 agggcgtgaa tgtaagcgtg acataactaa ttacatgact cgaggtcgac ggtatcgata
      301 agcttgatat cgaattcctg cagcccatca ccactttgta caagaaagct gggtttcaca
      361 attcatcatg atcatcgtag taataatcta acttggtgaa caccgttttg cagcctttat
      421 ctgagaaaac tctctcctct ttggggaaaa atgtcatctc ctcagccact ctgcttacaa
      481 tgtcttcatc aatttcatag cctcgggact gcatttccac tcggatacac atttttaggt
      541 gtttgtattc cagggggagg aagggaacaa aataatcaat gaggttccgg tcaattaagc
      601 tgctgtgcca gaagccactg ttcttgttat tgaaaaccga cacagacaac gcgtgttcaa
      661 tgtctttgag cttgatgtct tccctctgct ttccactcct ccagaaatcc aaagccacat
      721 ctgtgatcct ttctgctcca gcattgctga gaaatatgaa catggctttc tggt
//
