LOCUS       sequence-382120-       10504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-U6-crCHIKV-array-UTR-3xp3-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Dalla Benetta E, Lopez-Denman AJ, Li HH, Masri RA, Brogan DJ, Bui M,
            Yang T, Li M, Dunn M, Klein MJ, Jackson S, Catalan K, Blasdell KR, 
            Tng P, Antoshechkin I, Alphey LS, Paradkar PN, Akbari OS
  TITLE     Engineered Antiviral Sensor Targets Infected Mosquitoes.
  JOURNAL   CRISPR J. 2023 Dec;6(6):543-556. doi: 10.1089/crispr.2023.0056.
  PUBMED    38108518
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023"; journalName: "CRISPR J"; date: "2023-12"; 
            volume: "6"; issue: "6"; pages: "543-556"
COMMENT     Sequence Label: OA-1093B
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_RNA        6445..6480
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        6511..6546
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        6577..6612
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        6643..6678
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        6709..6744
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     regulatory      7635..7644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7774..7792)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8042..8061
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8236..8259
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9193..9314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9230..9249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9284..9303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9982..10016
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10197..10213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10197..10213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10210..10232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10221..10237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10245..10251,10252..10269,10270..10275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10290..10311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10428..10445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
     3241 aaacttttaa acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc
     3301 atgttgtatt ataaaataag taattagctt aacttataca taatagaaac aaattatact
     3361 tattagtcag tcagaaacaa ctttggcaca tatcaatatt atgctctcga caaataactt
     3421 ttttgcattt tttgcacgat gcatttgcct ttcgccttat tttagagggg cagtaagtac
     3481 agtaagtacg ttttttcatt actggctctt cagtactgtc atctgatgta ccaggcactt
     3541 catttggcaa aatattagag atattatcgc gcaaatatct cttcaaagta ggagcttcta
     3601 aacgcttacg cataaacgat gacgtcaggc tcatgtaaag gtttctcata aattttttgc
     3661 gactttgaac cttttctccc ttgctactga cattatggct gtatataata aaagaattta
     3721 tgcaggcaat gtttatcatt ccgtacaata atgccatagg ccacctattc gtcttcctac
     3781 tgcaggtcat cacagaacac atttggtcta gcgtgtccac tccgccttta gtttgattat
     3841 aatacataac catttgcggt ttaccggtac tttcgttgat agaagcatcc tcatcacaag
     3901 atgataataa gtataccatc ttagctggct tcggtttata tgagacgaga gtaaggggtc
     3961 cgtcaaaaca aaacatcgat gttcccactg gcctggagcg actgtttttc agtacttccg
     4021 gtatctcgcg tttgtttgat cgcacggttc ccacaatggt taattcgagc tcgcccgggc
     4081 tcctaggaga tctatatgtc ttgccgaagc acattaaccc aagccattta tatatcaagt
     4141 acaagttgat ttgattctag tgatggactg acacgaatct atggcactgt tgcagagctt
     4201 acaaacaggt acccggagtc gcttcaatta ttcaatcatc cattcaaaaa cttccatctc
     4261 ttccaattag ccaatattca aattggaatc cagccagccg acggaggcag atggtctacc
     4321 tggcaactat agagtttcca tgtttccaga ctttccctcc cggtaaacgg agacaaaacg
     4381 acagacgtaa gtaggtacat atgcataccg cacggacaaa tcaaatttgt ctggcagctc
     4441 caattagagt cgttaaaaat ttaacgatgc gctaaataac ttcaagctat ttgtctcgct
     4501 ggattggctt cgagtggtaa gatcctatca aatgccgaaa acaaaaaact ttcttcttaa
     4561 ttgttcgttc ttcaacacct ctccatggtg ataacggata cggtttcatt gtcagcatcc
     4621 atcctccgaa aaatacatta cgccttgaaa tatgcaatcg caaacacgga tctgtttgga
     4681 acatttattt tactatgaag agatgcgata ggtaatattt atttgagcgt ttaagatact
     4741 cattgttctc tcaaagaatg tcattgaaag ccaacgaggt caaatcaaat attataataa
     4801 aaaggtcaaa gaggactaac ttaaagctct ctttatggat aggaaaaaat attttcgccc
     4861 atcgctagaa cttttaccgt ttccattgag tatataacta agatgaatga ggctaattga
     4921 tgtttaaaca agatgcttcg gcaattaata ataaggaatg gtaaagcatt cttcaaatgt
     4981 tactatgttc tcaaattttc aagaattagg tattcaagaa attctttcag gcatattcca
     5041 taatctgcac aagcagctac tttagaaatt attgccgggc ttaaattata tgtacaacat
     5101 ttcctgagta ttttctgaca aaaattctca caagggaagg tcacgatgga gttacacggg
     5161 gtttttaacc ggactatttt tgttagattc tacatgtcga aatatgaaaa accccaaagc
     5221 ctttcgggtg atggaccagt ggtgtagcca ggaggggctc tgaaaggggt aattttgtac
     5281 gtatattata ttattaagct aattggaaat ttgacaaaaa tattttttta gtatttattg
     5341 tgatggtaga gaacaaaatt gacattaatg tacgtttgaa atgtatttgc catgcctttg
     5401 gcgtactaac atggagtatg gacaccaaaa ccgatttcgc gatcgcttaa ttaatcatca
     5461 caaacgaaac tattataatc gtcttcaggg gaacggtagc atcatatctg tcaccagtca
     5521 gtcggattcc tttctttcgg cataaattat gctcgggtga cgagacgagt tgaaaatccc
     5581 gtaaattaaa acatatacca cactcgccgt cctagacgac gacgacgacg acgattccca
     5641 aggaattgaa cccttcattt gcatttcact accgctctga gagatgcaaa gcgcgccaga
     5701 cagtcgatcg aatctgcaga ccagagagtc acatcaagaa tgttgacatt ccaccagaag
     5761 actctaatta ttcttcgctc gactgggaaa actgttaaca actgctcgag tgtggtcgga
     5821 gcggctcaca gtgcgttttc ccgtagacaa aaatcaaatt tcatggtatt tttcggcgat
     5881 aaacagtaag ttaaagggaa cccgaattta gtgctatata atttaattcc actagagttt
     5941 gtatcctttg atagatacgc gtatttcgac ctcaactgca aggccgtcgt gtactagact
     6001 tgactaatcc agactggtct tttagttatg acttctgtcc acatctccat acattcaacg
     6061 cactgtgcgg ctgtgctgtg cgactccgtc gagtcgacca acatagttga aacaaattga
     6121 atatttaatt gatcgttata ggaatggtgt tagatgagtc atcctttaca gtaagcacat
     6181 acagtattat aattgaagat cgtcggcaga taggtgtgta gggtagagta tcagcaataa
     6241 gttgggacgt ttgacttttt gtaggtagac aaaaactaaa ctttttttcg cttctctatg
     6301 tgtgcccctg ggtagcgttc cgttccgatt ggggtgcgaa cgaatgaaat cgcccatcga
     6361 gttgatacgt ccatccatcg ctagaaccgc gttcgctgta gaagactata taagagcaga
     6421 ggcaagagta gtgaaatgct agcgcaagta aacccctacc aactggtcgg ggtttgaaac
     6481 agtcagttct gcttctcgtt cttcctctgc caagtaaacc cctaccaact ggtcggggtt
     6541 tgaaacgttt cctttcggtg ggttctgcag cgtcttcaag taaaccccta ccaactggtc
     6601 ggggtttgaa actcatatct actgtgaacc tatccattgg tacaagtaaa cccctaccaa
     6661 ctggtcgggg tttgaaaccg aagttggatc tggagcttgc aaaggtggca agtaaacccc
     6721 taccaactgg tcggggtttg aaactttttt tgctagcggt ggttgttcat cgattcctaa
     6781 tattgcagtg aagcagtttg ttgtgcatgg cgaagataag gttagggcga ggaatgcctt
     6841 ggaaataagc ggcactgggc cgtatgaata aaaagcgttg aactttacta catgtagcat
     6901 ctactcaaaa acaaaatcca caaagtttac tacacttttg gtatgaataa aaaccttttc
     6961 gaacatgtag tacctactac tttaagaaaa ttctattcaa agtacagagt aatgctgcat
     7021 gttaagaaaa ttccattcag agtgactctt gaaatcaata aaatcatgca tatatgccaa
     7081 atttgtcact tattcacgtg acaaattatg tgaaaatatg aatattacta cattcccgct
     7141 atgcatcgcg taatcgatca tgaaatttat gttggttatg taactttaca gtattccctt
     7201 tatgtgcatg tgaattttat aatgatttga caacaaggat tttccttaga attttcgaat
     7261 tttagctaga aatctgtatc ctatgaatga atcttgagat aacagcaatt gtaacattat
     7321 aaagttccta acattttcaa gattccagta ttgaggcctt ttgggcccgg cgcgccaatt
     7381 cgagctcgcc cggggatcta attcaattag agactaattc aattagagct aattcaatta
     7441 ggatccaagc ttatcgattt cgaaccctcg accgccggag tataaataga ggcgcttcgt
     7501 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
     7561 gcaaataaac aagcgcagct gaacaagcta aacaatcggg gtaccgctag agtcgacggt
     7621 acgatccacc ggtcgccacc atggtgagca agggcgagga ggtcatcaaa gagttcatgc
     7681 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     7741 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     7801 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     7861 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     7921 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     7981 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     8041 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     8101 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     8161 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     8221 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     8281 agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc ggcagcaccg
     8341 gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtt atcaaggaat
     8401 ttatgcgctt caaagttagg atggagggat ccatgaacgg acatgagttc gagatcgagg
     8461 gagagggcga gggacgcccg tatgaaggca cacaaacagc caaactcaag gtcaccaagg
     8521 gcggaccact gcccttcgcc tgggatatcc tgagtcccca gtttatgtac ggcagcaagg
     8581 cctacgttaa gcaccccgct gacataccgg actacaaaaa gctgtccttt ccggaaggct
     8641 tcaagtggga gcgcgtgatg aatttcgaag acggaggact ggtcactgtg acccaagata
     8701 gcagtttgca ggacggtaca ctgatctata aggttaaaat gcgcggcact aactttccgc
     8761 cagatggccc agtgatgcag aagaagacca tgggttggga ggcatccacc gaacgtctgt
     8821 accctcgaga cggagtgctc aagggcgaga tccatcaggc cctcaaactg aaagatggtg
     8881 gtcactacct ggtcgaattt aagaccattt acatggccaa gaagccggtt cagctgcccg
     8941 gatattatta tgtggatacg aaactggata taacttcgca taacgaagac tacaccattg
     9001 tcgagcagta tgagcgcagc gaaggccgac atcacctgtt cctctacggc atggacgagc
     9061 tgtacaagta ggcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     9121 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     9181 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9241 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9301 atcaatgtat cttaaagctt ctaggccggc cgatctcgga tctgacaatg ttcagtgcag
     9361 agactcggct acgcctcgtg gactttgaag ttgaccaaca atgtttattc ttacctctaa
     9421 tagtcctctg tggcaaggtc aagattctgt tagaagccaa tgaagaacct ggttgttcaa
     9481 taacattttg ttcgtctaat atttcactac cgcttgacgt tggctgcact tcatgtacct
     9541 catctataaa cgcttcttct gtatcgctct ggacgtcatc ttcacttacg tgatctgata
     9601 tttcactgtc agaatcctca ccaacaagct cgtcatcgct ttgcagaaga gcagagagga
     9661 tatgctcatc gtctaaagaa ctacccattt tattatatat tagtcacgat atctataaca
     9721 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     9781 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     9841 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     9901 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     9961 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
    10021 aaaagatttg cgctttactc gacctaaact ttaaacacgt catagaatct tcgtttgaca
    10081 aaaaccacat tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt
    10141 cgcgcgagcg tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatgtca
    10201 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    10261 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    10321 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    10381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    10441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    10501 cggt
//
