LOCUS       sequence-382127-       12341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Site specific insertion using CRISPR/Cas9 editing of C. elegans 
            ChrI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Blazie SM, Takayanagi-Kiya S, McCulloch KA, Jin Y
  TITLE     Eukaryotic initiation factor EIF-3.G augments mRNA translation 
            efficiency to regulate neuronal activity.
  JOURNAL   Elife. 2021 Jul 29;10. pii: 68336. doi: 10.7554/eLife.68336.
  PUBMED    34323215
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-07-29"; volume: "10. pii: 68336. doi"; pages: " 
            10.7554/eLife.68336"
COMMENT     Sequence Label: pCZGY2727
FEATURES             Location/Qualifiers
     source          1..12341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(199..303)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     509..531
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     523..540
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     524..540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2085..2118
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    6923..6956
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    6986..7110
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7151..7456)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7253..7272)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(7798..8457)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8372..8391
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(8511..8517,8518..8535,8536..8541))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(8566..8690)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8706..8722)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8707..8723)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(10254..10270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10254..10270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10267..10289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10278..10294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10302..10308,10309..10326,10327..10332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10347..10368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10429..11017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10509..10528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      11128..11637
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11215..11234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11421..11442
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(11679..12341,1..129,130..198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12302..12321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cattgggaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
       61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      301 gcgcacattt ccccgaaaag tgccacctga cgtagttaac aaaaaaaagc ccgccgaagc
      361 gggctttatt accaagcgaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
      421 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
      481 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
      541 tgatcagtca tccaagtggg gatcaggaag aagttgataa agtgattcaa aaactcgagg
      601 agctcaacac agcgatgaac ccaattttga gagcaacact gacgaagaac tatgacgcga
      661 gggtaggcac taccttgaag ctatccacct gcctgcctgc cgcctatttc catgtgcagt
      721 aggaacgtgt gtaggtatag gcaggcatta tcgcgtctcc tttcgaacta cctagtacct
      781 agtaggtagt ttgaagggag gcgcgataat gcctgcctat gtctacacat gtgcctactg
      841 cgcatggaaa taggcggtag tcaggcaggt agatagcctc ataaaattca atacttcctt
      901 ttcagaaaga atgctggaat gtattcaaag aggagtcact cttcccattg ctcagagaat
      961 atgaagagtt gctcggcgag caaaagttca tgattggaga tcgatattca tgggctgaca
     1021 tcgctgtcgt cgagtttcta actcggtgtc agcagtgcta cgatagcttc tatctggctc
     1081 atttcgagag gctccgaact ttttgccaga cttttgagtc tcttccgcac attcgtccct
     1141 acattcaggg acgtgttgat tcatttatct aattttttaa aatggatatt tttcgttttt
     1201 tatttgtttg ttttgctgat aattcaataa agttacgcaa cgaattttag acatttatga
     1261 aaaccgaaaa tcgacaaaaa tattttaaga aaaatagcag caaaattcaa tattttatca
     1321 aattttgttt atggtaatga gaaagaaaac atacaatatt tttctaagaa gaagaaaaat
     1381 gaccagaatt ttaagatttt tctcgaatat acgaaaaatt gataaactaa aattttcgat
     1441 ttttccattt attttgaaaa aaaaactctt gacggttttc accatgcgaa gaggtgagaa
     1501 atattctgaa agttatgcat tttgaaaaaa ttgcaaaaaa atacgttaaa attttttgat
     1561 ctattgtttt ttttgaaaac cggaatttaa aatggaattt tttttcaaaa attaaaatta
     1621 aaatttaaat atttctagtg tttggatagt ttttcaaaag ctagatttaa cccgcgagat
     1681 gtcgaccgct agtgtagctt acagatggct gaagacttcg caaaaatctt atcttagaaa
     1741 cccgcgaggt gtcggccgct aaaactttgt aaacctgcca acaaaatgct tcgcattgtc
     1801 tgaacagatg aaaccttgaa acaaaacata tacaattgaa atcccgtaaa gtgtcgggcg
     1861 caagaactgc gattagttgt ataattggac tagctcaaat ttgaccaaaa tctcattttt
     1921 agacctctaa gatgtcggcc actataactt tattcaactt gaaactgcaa aaaaacttat
     1981 caactgtata gattcaagta ttatgtcaag tattggtttt agagctacag tacactctga
     2041 taacaaaatc tgcagaaata cctccctgtc aattcccaaa atacgaagtt cctattctct
     2101 agaaagtata ggaacttcgg gtgaaaggaa cgacactatg tacatcaacc agaaattaca
     2161 agatattgga gaaatatatt caaataaata ataaatacat cattgttaca atggagggcg
     2221 ttttgttgcc atatcaacca tttcgttatg ttgtcgaatt ggttgtgaat cccgtggctg
     2281 aaaaaattct taaggttatt aaaacattta tcatcgcata atacctttgg ctgaatatca
     2341 gaaattccgt ttcgactatg ttttggagga ctcgtaccaa cggggacagt tttgatggac
     2401 atcaatgttt tactgagttc caaaagattt tgagaaagta ctgcgcatag ctcactagat
     2461 ttcgcatttc gaacatcctc ggcatatatt tctgaaaaat gattttggaa ttcaaagaat
     2521 ttgtaacatt atattggcta aaagatttga aggttgataa tatccattac ctaatgcctt
     2581 agaaacttga caacacatca tatcctgttc attcagtcga gtctggagag ttgaaacctg
     2641 ctcttccatc atttgtactt catttgccca gttatctttc ttttggggag tcctcaatgg
     2701 ttctcttagc ggctcatttg atctccgtgg tgaagatgga cccgaaacgg tgttcgaacg
     2761 atcacgaatt gtgttcttca ctttttgaaa tactggagca agcgcgagaa tggttgaggg
     2821 tgactctgcg ccatcattct ttgaattaac tgctgctgga ggtctttgag gacgttgagt
     2881 tctgtttgga tgactgcttc cagcaaccgt gatatgtgaa aagttgtttg attttatcat
     2941 tggttttgca tttttctgaa aaaaatatta ttttttatga ctatgtttca tttaatatac
     3001 tttaggattc ctatagtgtc tggctaattt cacgaatgag tcgatatctg ctatcggagg
     3061 atatctcatg agatattgaa gacatccacc gtagtctgat gacaatactg taaagaagct
     3121 ttgtcttcaa attttttatc gatttaacaa cttacaaaga tgtctgatct gaactaacaa
     3181 cgaaacaaac atgcatttgg ctaatggggc aattggccga tcaatcagaa gtacatccca
     3241 tagaaaaagc aaatcgtgta gaggaagttc tcggccaaat aataagcgaa gccaacgact
     3301 gtaaatattt taattaaata ttttcaaaaa taaaataact cacattccat aaagttgtgg
     3361 tggtatatca agagtactaa gatgtttggc aagggtggga tcgatttcgt gtagaagatt
     3421 tccaatatca attagatctt ccatcaaacg agatgcagga gcggaatctt gcaccctcat
     3481 gtacggttcc tttggaattg gacgctttgg actctcagtg actgttggtt cttcatacca
     3541 tcttgatact tctaacatca ccgaacaaaa cagattacta aaaatatatt atcaataatg
     3601 attgaaattt attcactcac tagctatcct gttcgaggta ttctttacaa aataagcaat
     3661 tcaaaatatc ttcttcttcc accgttagca ttttcaactc atcattctct tttgcatgtt
     3721 gaaatgcttc attgtccgaa tatatcacga aaattagcgg tgccagaatt tcgtgcattc
     3781 cttgacgata atttacaaaa ggatgctcct ttgcataaac caaaagaata tctgacatca
     3841 tttgccgaat acttgtgttt tgaaagaact caatttccgg aaaagttcgt gaaacatctt
     3901 tgccgataat gtcacgaaga tcattgtcct cgaaaaatgt attccatgga ttttgctcaa
     3961 tagaggcaag tggattgttg aattcaggat cttgagaaaa ctttgtatcg tgtgggtcaa
     4021 ttaaatgatt ttccttgtga gctcggtaaa gattccgact acgtgataag cttatttccc
     4081 aatcacttgt ttcatacggc aaacatcgaa gaacaagctg taatcaattg acttttgaag
     4141 actaatttag aaatgccgaa actaataccc tccaaacagc tgatctacat gaagatgaac
     4201 gcaaggagcc tgatgcagcg actcttgcta ggagatcatc agaaagctta tatttgatta
     4261 gtgacgtcgc atcaaagctg aacacataaa ttctttaata ttttatatcc ccatattatg
     4321 cacctgagag gaatgaagaa atttgctgat actcccggtg aattcatgtc ttgatggttg
     4381 tgcgcattgt ggtaatatct gaaaaatttc gaagtcagtt ttctgataag gcaacaacta
     4441 ataaattgag aatcaattta cccttaagga acgatgttcg cttctgaatt tattaataaa
     4501 tttttccgta attcaattca aaattgtcat atttaagaaa aaaacatcaa aaatgacaaa
     4561 ttcaatattt taattaattt ttgctttcgt cgtaaatcta cacacgcgtc tcttccgtgc
     4621 gagagtccaa gccagcagcc aaattcgttg actgagtatt caacgtttat acgttgtcgg
     4681 caacgagaaa taggaaaatg catcgggaaa tgttcttttt tcgatttttt ccaaggtttt
     4741 gacaaatttt accacgaatt ttgctatgtt ttcaattaaa aaatatgtta ttcaactgtt
     4801 tctatgagga aaataaggct ttgcatgtaa ttttcttatt cagcataatt tttaattaat
     4861 ttgaattttc tgtcctaacg tttattttgt tttcttggtt atgactgatc tgaaattaat
     4921 ttttgaattt taaggtaata tgaaaaaacc cgagttgacc gccacatccg tagagaagtt
     4981 cctcatcgag aagttcgact ccgtctccga cctcatgcaa ctctccgagg gagaggagtc
     5041 ccgtgccttc tccttcgacg tcggaggacg tggatacgtc ctccgtgtca actcctgcgc
     5101 cgacggattc tacaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa
     5161 ttttcaggac cgttacgtct accgtcactt cgcctccgcc gccctcccaa tcccagaggt
     5221 cctcgacatc ggagagttct ccgagtccct cacctactgc atctcccgtc gtgcccaagg
     5281 agtcaccctc caagacctcc cagagaccga gctcccagcc gtcctccaac cagtcgccga
     5341 ggccatggac gccatcgccg ccgccgacct ctcccaaacc tccggattcg gaccattcgg
     5401 accacaagga atcggacaat acaccacctg gcgtgacttc atctgcgcca tcgccgaccc
     5461 acacgtctac cactggcaaa ccgtcatgga cgacaccgtc tccgcctccg tcgcccaagc
     5521 cctcgacgag ctcatgctct gggccgagga ctgcccagag gtccgtcacc tcgtccacgc
     5581 cgacttcgga tccaacaacg tcctcaccga caacggacgt atcaccgccg tcatcgactg
     5641 gtccgaggcc atgttcggag actcccaata cgaggtcgcc aacatcttct tctggcgtcc
     5701 atggctcgcc tgcatggagc aacaaacccg ttacttcgag cgtcgtcacc cagagctcgc
     5761 cggatcccca cgtctccgtg cctacatgct ccgtatcgga ctcgaccaac tctaccaatc
     5821 cctcgtcgac ggaaacttcg acgacgccgc ctgggcccaa ggacgttgcg acgccatcgt
     5881 ccgttccgga gccggaaccg tcggacgtac ccaaatcgcc cgtcgttccg ccgccgtctg
     5941 gaccgacgga tgcgtcgagg tcctcgccga ctccggaaac cgtcgtccat ccacccgtcc
     6001 acgtgccaag gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     6061 ggagtaaaat tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa
     6121 taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat
     6181 atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa
     6241 catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa
     6301 cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg
     6361 tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc
     6421 ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt
     6481 tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat
     6541 catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc
     6601 atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc
     6661 aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt
     6721 tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     6781 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     6841 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     6901 aatataccaa acataactgt ttgaagttcc tattctctag aaagtatagg aacttcgcat
     6961 gccgggctgc aggaattcga tatcaaccac tttgtacaag aaagctgaac gagaaacgta
     7021 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     7081 ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg tgtataaggg
     7141 agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc
     7201 ggtggctgag atcagccact tcttccccga taacggagac cggcacactg gccatatcgg
     7261 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga
     7321 ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
     7381 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
     7441 aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca
     7501 ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc
     7561 agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat
     7621 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
     7681 catacctctt tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa
     7741 atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc cagatcttta
     7801 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     7861 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     7921 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     7981 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     8041 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     8101 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     8161 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     8221 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
     8281 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     8341 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     8401 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     8461 agcttcctta gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga
     8521 gccggaagca taaagtgtaa agcctggggt gcctaatgcg gccgccatag tgactggata
     8581 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     8641 atatttatat cattttacgt ttctcgttca gcttttttgt acaaacttgt tgatatcaag
     8701 cttatcgata ccgtcgacct cgagacgtac ggtgcgcgcg atgcattcga agatctgccc
     8761 actagtaagt cggcgatttc tttgaagttt tgtcaaaaga agagacaagg acttgataga
     8821 tacgctcatc agagtgagat tcattgcaaa tagtgtgtaa atcgtggaga gacgagagaa
     8881 aaggtgtgag aagcttagaa gataaagaat aggaagaaga acatatctca tttcctattt
     8941 tctctaattt ttcgttcatt tttctagaca ttacccattt gatggttttt caatatggtt
     9001 atcagggtac tcattttggt agttttcaaa gcaatttgga gttttttttt aaattgtcaa
     9061 ctacaaaaac gcagcttaaa ggtccaaata tcatagtata aaaattcttt aaaaaattcc
     9121 aaattttata tgattttttg aaaattggaa gaatgttagt ttttgccatt tttttggaag
     9181 tttttctccg gattttatgg tacttatatt tcaaatataa atttttgtag gaattcgagt
     9241 atgcgagaga gctatagttt actaaaaaaa agtggcaaaa actcagtaat tgccactttt
     9301 ttcaaaaatg ttttgaaaaa ttttactatg atatttggcc attttggcac catatgtacg
     9361 ttacttttta cgaaacactc taatttttga aatttgagtt tcatgtttaa tcttttgtat
     9421 aaaattttta tggtcctttt gtgacaggat aaaaaattgc aaaaaaatag aaaactttgt
     9481 ttcgtgaaaa cacctagttt aaaaattgtg ggccacttag cgattctagt caaaaatcag
     9541 acaaatgtca gctgccgtaa ctttttccgg atcagggtta tcaaatttta attttaattg
     9601 taaatgtaga aaaatgatcg gcaacatttt gctaattgaa aatattttga aaattgctat
     9661 agtacatatt aatctagtat taatatagta ttaatgttat aatatcagtt atgtgtttga
     9721 ataactcaag cgtaagtttc cgtaaaatat ctttaaattt tggtaatttt ttacggtttg
     9781 gtataaattt ttgcattttt tgattttcta cacttttttt cgatttcgca ttttccgaat
     9841 ttctctgaaa taaatcgaac aatcggaaaa ttttcgaggg cacccttagc catgcagctc
     9901 aggaatttga ctttatgttc ccattcaaat ttttttaaaa ttaaataaaa tgctaattgt
     9961 aagataaatt acaaaaatta ttaaatctca tcatccttat gatgatagtg cagtgcatga
    10021 atatattttt ccaaatagga tatgattcgt cgagtagctt cacgtaatcc gttttcttca
    10081 atcaccattt cgaactgcaa tttttttttt ggatttttgt aacaatattg tcataaattg
    10141 tgattttgat attttgcgta gagcatcaga acctttctgc tttgaatttt aatgggatta
    10201 aaaattataa aaaacttgca ccctaattaa agcgcgatac ctggtgaccc atggtcatag
    10261 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    10321 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaca tgtgagcaaa
    10381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11101 aaaaaggatc ttcacctaga tccttttacg cgccctgtag cggcgcatta agcgcggcgg
    11161 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    11221 tcgctttctt cccttccttt ctcgccacgt tcgctttccc cgtcaagctc taaatcgggg
    11281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
    11341 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    11401 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    11461 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    11521 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaattta
    11581 aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa ccggggtaaa
    11641 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11701 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11761 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11821 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11881 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11941 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12001 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12061 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
    12301 gataataccg cgccacatag cagaacttta aaagtgctca t
//
