LOCUS       sequence-382129-        3429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_69.11
FEATURES             Location/Qualifiers
     source          1..3429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      66..160
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(191..196,197..213,214..219)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             427..960
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      1113..1701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1602..1621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1855..1872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1887..2027
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1953..1972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2113..2135
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2129..2320)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2500..2586)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2658..2692
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2701..2712
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctcgggtaag aactcgcact tcgtggaaac actattatct ggtgggtctc tgtccactag
       61 tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
      121 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta
      181 cgatttaaat ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg
      241 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
      301 tttcatggct tgttatgact gtttttttgt acagcctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
      481 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
      541 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc cacctactcc
      601 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
      661 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
      721 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg agagcaccgg
      781 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
      841 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
      901 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
      961 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attggacaac
     1021 ggtcggctcg gcccacttgt gtgtctcgta caattctgag gtccacacag atagtgtccg
     1081 gccggccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1141 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1201 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     1261 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1321 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1381 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1441 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1501 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1561 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1621 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1681 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1741 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1801 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1861 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     1921 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1981 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     2041 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     2101 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     2161 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct
     2221 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     2281 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     2341 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     2401 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     2461 ggatgcggcg ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     2521 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     2581 tatttgatgc ctttaattaa ggctcgggag acctatcggt aataacagtc caatctggtg
     2641 taacttcgga atcgtccttt acggctagct cagtcctagg tatagtgcta gctactagtg
     2701 aaagaggaga aatactagta tggttagcaa aggcgaggcg gttatcaagg agtttatgcg
     2761 ttttaaggtt cacatggagg gtagcatgaa tggtcacgag ttcgagatcg agggtgaagg
     2821 cgagggtcgt ccgtacgaag gcacccagac cgcgaagctg aaagtgacca agggtggccc
     2881 gctgccgttc agctgggaca tcctgagccc gcagttcatg tatggcagcc gtgcgtttac
     2941 caaacacccg gcggacattc cggattacta taagcaaagc ttcccggaag gttttaaatg
     3001 ggagcgtgtt atgaacttcg aagatggtgg cgcggtgacc gttacccagg acaccagcct
     3061 ggaggatggc accctgattt acaaggtgaa actgcgtggc accaactttc cgccggatgg
     3121 tccggttatg cagaagaaaa cgatgggttg ggaagcgagc accgagcgtc tgtatccgga
     3181 agatggcgtg ctgaagggtg atatcaaaat ggcgctgcgt ctgaaggacg gtggccgtta
     3241 cctggcggat tttaagacca cctataaagc gaagaaaccg gtgcaaatgc cgggtgcgta
     3301 caacgttgac cgtaaactgg atattaccag ccacaacgag gattataccg tggttgagca
     3361 atatgagcgt agcgagggtc gccacagcac cggcggcatg gacgaactgt ataagggatc
     3421 ctaataagg
//
