LOCUS       sequence-382131-        2776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_66.11
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      66..160
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(191..196,197..213,214..219)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             427..960
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      1124..1669
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1570..1589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1786..1803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1847..1933)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2005..2039
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             2048..2059
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctcgggtaag aactcgcact tcgtggaaac actattatct ggtgggtctc tgtccactag
       61 tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
      121 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta
      181 cgatttaaat ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg
      241 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
      301 tttcatggct tgttatgact gtttttttgt acagcctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
      481 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
      541 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc cacctactcc
      601 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
      661 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
      721 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg agagcaccgg
      781 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
      841 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
      901 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
      961 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attggacaac
     1021 ggtcggctcg gcccacttgt gtgtctcgta caattctgag gtccacacag atagtgtccg
     1081 gccggcccta gaaatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc
     1141 tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg
     1201 ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa
     1261 aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat
     1321 caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga
     1381 cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     1441 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg
     1501 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     1561 gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt
     1621 tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc
     1681 cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc
     1741 cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc
     1801 gaggaagcgg aatatatccg gcgcgcccag ctgtctaggg cggcggattt gtcctactca
     1861 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     1921 ttcgttttat ttgatgcctt taattaaggc tcgggagacc tatcggtaat aacagtccaa
     1981 tctggtgtaa cttcggaatc gtccttgaca gctagctcag tcctaggtat aatgctagct
     2041 actagtgaaa gaggagaaat actagtatgg ttagcaaagg cgaggcggtt atcaaggagt
     2101 ttatgcgttt taaggttcac atggagggta gcatgaatgg tcacgagttc gagatcgagg
     2161 gtgaaggcga gggtcgtccg tacgaaggca cccagaccgc gaagctgaaa gtgaccaagg
     2221 gtggcccgct gccgttcagc tgggacatcc tgagcccgca gttcatgtat ggcagccgtg
     2281 cgtttaccaa acacccggcg gacattccgg attactataa gcaaagcttc ccggaaggtt
     2341 ttaaatggga gcgtgttatg aacttcgaag atggtggcgc ggtgaccgtt acccaggaca
     2401 ccagcctgga ggatggcacc ctgatttaca aggtgaaact gcgtggcacc aactttccgc
     2461 cggatggtcc ggttatgcag aagaaaacga tgggttggga agcgagcacc gagcgtctgt
     2521 atccggaaga tggcgtgctg aagggtgata tcaaaatggc gctgcgtctg aaggacggtg
     2581 gccgttacct ggcggatttt aagaccacct ataaagcgaa gaaaccggtg caaatgccgg
     2641 gtgcgtacaa cgttgaccgt aaactggata ttaccagcca caacgaggat tataccgtgg
     2701 ttgagcaata tgagcgtagc gagggtcgcc acagcaccgg cggcatggac gaactgtata
     2761 agggatccta ataagg
//
