LOCUS       sequence-382138-        4786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged Rab11 in mammalian cells and zebrafish 
            embryos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Aljiboury AA, Ingram E, Krishnan N, Ononiwu F, Pal D, Manikas J, 
            Taveras C, Hall NA, Da Silva J, Freshour J, Hehnly H
  TITLE     Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during 
            zebrafish left-right organizer development.
  JOURNAL   PLoS Genet. 2023 May 15;19(5):e1010765. doi: 
            10.1371/journal.pgen.1010765. eCollection 2023 May.
  PUBMED    37186603
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-05-15"; volume: "19(5):e1010765. doi"; pages: "
            10.1371/journal.pgen.1010765. eCollection 2023 May"
COMMENT     Sequence Label: pCS2-FLAG-Rab11
FEATURES             Location/Qualifiers
     source          1..4786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             111..134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    838..972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(862..881)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     916..935
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1085..1105)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1085..1103)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1124..1140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1124..1140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1137..1159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1148..1164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1172..1178,1179..1196,1197..1202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1217..1238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1355..1372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1526..2114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1606..1625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2285..3076,3077..3145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2908..2927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3146..3250)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3276..3731)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3413..3434)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3625..3644
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3802..4786
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg tcagatccga gacccaagct ggctagcatg gactacaaag
      121 acgatgacga caagctcaag cttgctcata agggcacccg cgacgacgag tacgactacc
      181 tctttaaagt tgtccttatt ggagattctg gtgttggaaa gagtaatctc ctgtctcgat
      241 ttactcgaaa tgagtttaat ctggaaagca agagcaccat tggagtagag tttgcaacaa
      301 gaagcatcca ggttgatgga aaaacaataa aggcacagat atgggacaca gcagggcaag
      361 agcgatatcg agctataaca tcagcatatt atcgtggagc tgtaggtgcc ttattggttt
      421 atgacattgc taaacatctc acatatgaaa atgtagagcg atggctgaaa gaactgagag
      481 atcatgctga tagtaacatt gttatcatgc ttgtgggcaa taagagtgat ctacgtcatc
      541 tcagggcagt tcctacagat gaagcaagag cttttgcaga aaagaatggt ttgtcattca
      601 ttgaaacttc ggccctagac tctacaaatg tagaagctgc ttttcagaca attttaacag
      661 agatttaccg cattgtttct cagaagcaaa tgtcagacag acgcgaaaat gacatgtctc
      721 caagcaacaa tgtggttcct attcatgttc caccaaccac tgaaaacaag ccaaaggtgc
      781 agtgctgtca gaacatctag gcctgcagtc tagaactata gtgagtcgta ttacgtagat
      841 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      901 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      961 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1021 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt
     1081 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     1141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta
     1201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3241 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     3301 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3361 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3421 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3481 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3541 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3601 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3661 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3721 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3781 ggcctcttcg ctattacgcc agtcgaccgc caattcaata tggcgtatat ggactcatgc
     3841 caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc
     3901 caattcaata tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc
     3961 caattcaata tggcggacct ggcactgtgc caactgggga ggggtctact tggcacggtg
     4021 ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg
     4081 ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat
     4141 ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata
     4201 tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc
     4261 atataccata tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg
     4321 gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc
     4381 gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt
     4441 caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc
     4501 tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct
     4561 attaatagta acttggcaag tacattacta ttggaagtac gccagggtac attggcagta
     4621 ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg
     4681 acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag
     4741 gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaac
//
