LOCUS       sequence-38216-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Bhargava K, Templeton P, Spremulli LL
  TITLE     Expression and characterization of isoform 1 of human mitochondrial 
            elongation factor G.
  JOURNAL   Protein Expr Purif. 2004 Oct . 37(2):368-76.
  PUBMED    15358359
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2004 Oct . 37(2):368-76."
COMMENT     Sequence Label: pET21c(+)-EFG1mt
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgtctt catcaggggt gattcctaat
       61 gaaaaaatac gaaatattgg aatctcagct cacattgatt ctgggaaaac tacattaaca
      121 gaacgagtcc tttactacac tggcagaatt gcaaagatgc atgaggtgaa aggtaaagat
      181 ggagttggtg ctgtcatgga ttccatggaa ctagagagac aaagaggaat cactattcag
      241 tcagcagcca cttacaccat gtggaaagat gtcaatatta acattataga tactcctggg
      301 catgtggact tcacaataga agtggaaagg gccctgagag tgttggatgg tgcagtcctt
      361 gttctctgtg ctgttggagg ggtacagtgc cagaccatga ctgtcaatcg tcagatgaag
      421 cgctacaacg ttccgtttct aacttttatt aacaaattgg accgaatggg ctccaaccca
      481 gccagggccc tgcagcaaat gaggtctaaa ctaaatcata atgcagcgtt tatgcagata
      541 cccatgggtt tggagggtaa ttttaaaggt attgtagatc ttattgagga acgagccatc
      601 tattttgatg gagactttgg tcagattgtt cgatatggtg agattccagc tgaattaagg
      661 gcggcggcca ctgaccaccg gcaggagcta attgaatgtg ttgccaattc a
//
