LOCUS       sequence-382170-       12727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human full-length LRRK2 with FKBP domain and 3xFLAG 
            situated at the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Kluss JH, Beilina A, Williamson CD, Lewis PA, Cookson MR, 
            Bonet-Ponce L
  TITLE     Lysosomal positioning regulates Rab10 phosphorylation at LRRK2(+) 
            lysosomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2205492119. doi: 
            10.1073/pnas.2205492119. Epub 2022 Oct 18.
  PUBMED    36256825
REFERENCE   2  (bases 1 to 12727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2205492119. 
            doi"; pages: " 10.1073/pnas.2205492119. Epub 2022 Oct 18"
COMMENT     Sequence Label: 3xFLAG-FKBP-LRRK2
FEATURES             Location/Qualifiers
     source          1..12727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1009..1329
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     protein_bind    1369..1393
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9010..9034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9068..9690
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(9267..9286)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(9714..9734)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9719..10048
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9899..10034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10086..10105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10087..10105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10119..10135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10119..10135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10132..10154)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10143..10159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10167..10173,10174..10191,10192..10197))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10212..10233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10350..10367)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10521..11109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10601..10620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11280..12071,12072..12140))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11903..11922
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12141..12245)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12272..12700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12359..12378)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12569..12590
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagggag gcagcggtgg agtgcaggtg
     1021 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
     1081 cactacaccg ggatgcttga agatggaaag aaatttgatt cctcccggga cagaaacaag
     1141 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
     1201 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
     1261 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
     1321 aaactggaaa agcttgcggc cgcgaattca tcgatagatc tgatatcaac aagtttgtac
     1381 aaaaaagcag gctccgaatt cgcccttatg gctagtggca gctgtcaggg gtgcgaagag
     1441 gacgaggaaa ctctgaagaa gttgatagtc aggctgaaca atgtccagga aggaaaacag
     1501 atagaaacgc tggtccaaat cctggaggat ctgctggtgt tcacgtactc cgagcacgcc
     1561 tccaagttat ttcaaggcaa aaatatccat gtgcctctgt tgatcgtctt ggactcctat
     1621 atgagagtcg cgagtgtgca gcaggtgggt tggtcacttc tgtgcaaatt aatagaagtc
     1681 tgtccaggta caatgcaaag cttaatggga ccccaggatg ttggaaatga ttgggaagtc
     1741 cttggtgttc accaattgat tcttaaaatg ctaacagttc ataatgccag tgtaaacttg
     1801 tcagtgattg gactgaagac cttagatctc ctcctaactt caggtaaaat caccttgctg
     1861 atattggatg aagaaagtga tattttcatg ttaatttttg atgccatgca ctcatttcca
     1921 gccaatgatg aagtccagaa acttggatgc aaagctttac atgtgctgtt tgagagagtc
     1981 tcagaggagc aactgactga atttgttgag aacaaagatt atatgatatt gttaagtgcg
     2041 ttaacaaatt ttaaagatga agaggaaatt gtgcttcatg tgctgcattg tttacattcc
     2101 ctagcgattc cttgcaataa tgtggaagtc ctcatgagtg gcaatgtcag gtgttataat
     2161 attgtggtgg aagctatgaa agcattccct atgagtgaaa gaattcaaga agtgagttgc
     2221 tgtttgctcc ataggcttac attaggtaat tttttcaata tcctggtatt aaacgaagtc
     2281 catgagtttg tggtgaaagc tgtgcagcag tacccagaga atgcagcatt gcagatctca
     2341 gcgctcagct gtttggccct cctcactgag actattttct taaatcaaga tttagaggaa
     2401 aagaatgaga atcaagagaa tgatgatgag ggggaagaag ataaattgtt ttggctggaa
     2461 gcctgttaca aagcattaac gtggcataga aagaacaagc acgtgcagga ggccgcatgc
     2521 tgggcactaa ataatctcct tatgtaccaa aacagtttac atgagaagat tggagatgaa
     2581 gatggccatt tcccagctca tagggaagtg atgctctcca tgctgatgca ttcttcatca
     2641 aaggaagttt tccaggcatc tgcgaatgca ttgtcaactc tcttagaaca aaatgttaat
     2701 ttcagaaaaa tactgttatc aaaaggaata cacctgaatg ttttggagtt aatgcagaag
     2761 catatacatt ctcccgaagt ggctgaaagt ggctgtaaaa tgctaaatca tctttttgaa
     2821 ggaagcaaca cttccctgga tataatggca gcagtggtcc ccaaaatact aacagttatg
     2881 aaacgtcatg agacatcatt accagtgcag ctggaggcgc ttcgagctat tttacatttt
     2941 atagtgcctg gcatgccaga agaatccagg gaggatacag aatttcatca taagctaaat
     3001 atggttaaaa aacagtgttt caagaatgat attcacaaac tggtcctagc agctttgaac
     3061 aggttcattg gaaatcctgg gattcagaaa tgtggattaa aagtaatttc ttctattgta
     3121 cattttcctg atgcattaga gatgttatcc ctggaaggtg ctatggattc agtgcttcac
     3181 acactgcaga tgtatccaga tgaccaagaa attcagtgtc tgggtttaag tcttatagga
     3241 tacttgatta caaagaagaa tgtgttcata ggaactggac atctgctggc aaaaattctg
     3301 gtttccagct tataccgatt taaggatgtt gctgaaatac agactaaagg atttcagaca
     3361 atcttagcaa tcctcaaatt gtcagcatct ttttctaagc tgctggtgca tcattcattt
     3421 gacttagtaa tattccatca aatgtcttcc aatatcatgg aacaaaagga tcaacagttt
     3481 ctaaacctct gttgcaagtg ttttgcaaaa gtagctatgg atgattactt aaaaaatgtg
     3541 atgctagaga gagcgtgtga tcagaataac agcatcatgg ttgaatgctt gcttctattg
     3601 ggagcagatg ccaatcaagc aaaggaggga tcttctttaa tttgtcaggt atgtgagaaa
     3661 gagagcagtc ccaaattggt ggaactctta ctgaatagtg gatctcgtga acaagatgta
     3721 cgaaaagcgt tgacgataag cattgggaaa ggtgacagcc agatcatcag cttgctctta
     3781 aggaggctgg ccctggatgt tgccaacaat agcatttgcc ttggaggatt ttgtatagga
     3841 aaagttgaac cttcttggct tggtccttta tttccagata agacttctaa tttaaggaaa
     3901 caaacaaata tagcatctac actagcaaga atggtgatca gatatcagat gaaaagtgct
     3961 gtggaagaag gaacagcctc aggcagcgat ggaaattttt ctgaagatgt gctgtctaaa
     4021 tttgatgaat ggacctttat tcctgactct tctatggaca gtgtgtttgc tcaaagtgat
     4081 gacctggata gtgaaggaag tgaaggctca tttcttgtga aaaagaaatc taattcaatt
     4141 agtgtaggag aattttaccg agatgccgta ttacagcgtt gctcaccaaa tttgcaaaga
     4201 cattccaatt ccttggggcc catttttgat catgaagatt tactgaagcg aaaaagaaaa
     4261 atattatctt cagatgattc actcaggtca tcaaaacttc aatcccatat gaggcattca
     4321 gacagcattt cttctctggc ttctgagaga gaatatatta catcactaga cctttcagca
     4381 aatgaactaa gagatattga tgccctaagc cagaaatgct gtataagtgt tcatttggag
     4441 catcttgaaa agctggagct tcaccagaat gcactcacga gctttccaca acagctatgt
     4501 gaaactctga agagtttgac acatttggac ttgcacagta ataaatttac atcatttcct
     4561 tcttatttgt tgaaaatgag ttgtattgct aatcttgatg tctctcgaaa tgacattgga
     4621 ccctcagtgg ttttagatcc tacagtgaaa tgtccaactc tgaaacagtt taacctgtca
     4681 tataaccagc tgtcttttgt acctgagaac ctcactgatg tggtagagaa actggagcag
     4741 ctcattttag aaggaaataa aatatcaggg atatgctccc ccttgagact gaaggaactg
     4801 aagattttaa accttagtaa gaaccacatt tcatccctat cagagaactt tcttgaggct
     4861 tgtcctaaag tggagagttt cagtgccaga atgaattttc ttgctgctat gcctttcttg
     4921 cctccttcta tgacaatcct aaaattatct cagaacaaat tttcctgtat tccagaagca
     4981 attttaaatc ttccacactt gcggtcttta gatatgagca gcaatgatat tcagtaccta
     5041 ccaggtcccg cacactggaa atctttgaac ttaagggaac tcttatttag ccataatcag
     5101 atcagcatct tggacttgag tgaaaaagca tatttatggt ctagagtaga gaaactgcat
     5161 ctttctcaca ataaactgaa agagattcct cctgagattg gctgtcttga aaatctgaca
     5221 tctctggatg tcagttacaa cttggaacta agatcctttc ccaatgaaat ggggaaatta
     5281 agcaaaatat gggatcttcc tttggatgaa ctgcatctta actttgattt taaacatata
     5341 ggatgtaaag ccaaagacat cataaggttt cttcaacagc gattaaaaaa ggctgtgcct
     5401 tataaccgaa tgaaacttat gattgtggga aatactggga gtggtaaaac caccttattg
     5461 cagcaattaa tgaaaaccaa gaaatcagat cttggaatgc aaagtgccac agttggcata
     5521 gatgtgaaag actggcctat ccaaataaga gacaaaagaa agagagatct cgtcctaaat
     5581 gtgtgggatt ttgcaggtcg tgaggaattc tatagtactc atccccattt tatgacgcag
     5641 cgagcattgt accttgctgt ctatgacctc agcaagggac aggctgaagt tgatgccatg
     5701 aagccttggc tcttcaatat aaaggctcgc gcttcttctt cccctgtgat tctcgttggc
     5761 acacatttgg atgtttctga tgagaagcaa cgcaaagcct gcatgagtaa aatcaccaag
     5821 gaactcctga ataagcgagg gttccctgcc atacgagatt accactttgt gaatgccacc
     5881 gaggaatctg atgctttggc aaaacttcgg aaaaccatca taaacgagag ccttaatttc
     5941 aagatccgag atcagcttgt tgttggacag ctgattccag actgctatgt agaacttgaa
     6001 aaaatcattt tatcggagcg taaaaatgtg ccaattgaat ttcccgtaat tgaccggaaa
     6061 cgattattac aactagtgag agaaaatcag ctgcagttag atgaaaatga gcttcctcac
     6121 gcagttcact ttctaaatga atcaggagtc cttcttcatt ttcaagaccc agcactgcag
     6181 ttaagtgact tgtactttgt ggaacccaag tggctttgta aaatcatggc acagattttg
     6241 acagtgaaag tggaaggttg tccaaaacac cctaagggaa ttatttcgcg tagagatgtg
     6301 gaaaaatttc tttcaaaaaa aaggaaattt ccaaagaact acatgtcaca gtattttaag
     6361 ctcctagaaa aattccagat tgctttgcca ataggagaag aatatttgct ggttccaagc
     6421 agtttgtctg accacaggcc tgtgatagag cttccccatt gtgagaactc tgaaattatc
     6481 atccgactat atgaaatgcc ttattttcca atgggatttt ggtcaagatt aatcaatcga
     6541 ttacttgaga tttcacctta catgctttca gggagagaac gagcacttcg cccaaacaga
     6601 atgtattggc gacaaggcat ttacttaaat tggtctcctg aagcttattg tctggtagga
     6661 tctgaagtct tagacaatca tccagagagt ttcttaaaaa ttacagttcc ttcttgtaga
     6721 aaaggctgta ttcttttggg ccaagttgtg gaccacattg attctctcat ggaagaatgg
     6781 tttcctgggt tgctggagat tgatatttgt ggtgaaggag aaactctgtt gaagaaatgg
     6841 gcattatata gttttaatga tggcgaagaa catcaaaaaa tcttacttga tgacttgatg
     6901 aagaaagcag aggaaggaga tctcttagta aatccagatc aaccaaggct caccattcca
     6961 atatctcaga ttgcccctga cttgattttg gctgacctgc ctagaaatat tatgttgaat
     7021 aatgatgagt tggaatttga acaagctcca gagtttctcc taggtgatgg cagttttgga
     7081 tcagtttacc gagcagccta tgaaggagaa gaagtggctg tgaagatttt taataaacat
     7141 acatcactca ggctgttaag acaagagctt gtggtgcttt gccacctcca ccaccccagt
     7201 ttgatatctt tgctggcagc tgggattcgt ccccggatgt tggtgatgga gttagcctcc
     7261 aagggttcct tggatcgcct gcttcagcag gacaaagcca gcctcactag aaccctacag
     7321 cacaggatcg cactccacgt agctgatggt ttgagatacc tccactcagc catgattata
     7381 taccgagacc tgaaacccca caatgtgctg cttttcacac tgtatcccaa tgctgccatc
     7441 atcgcaaaga ttgctgacta cggcattgct cagtactgct gtagaatggg gataaaaaca
     7501 tcagagggca caccagggtt tcgtgcacct gaagttgcca gaggaaatgt catttataac
     7561 caacaggctg atgtttattc atttggttta ctactctatg acattttgac aactggaggt
     7621 agaatagtag agggtttgaa gtttccaaat gagtttgatg aattagaaat acaaggaaaa
     7681 ttacctgatc cagttaaaga atatggttgt gccccatggc ctatggttga gaaattaatt
     7741 aaacagtgtt tgaaagaaaa tcctcaagaa aggcctactt ctgcccaggt ctttgacatt
     7801 ttgaattcag ctgaattagt ctgtctgacg agacgcattt tattacctaa aaacgtaatt
     7861 gttgaatgca tggttgctac acatcacaac agcaggaatg caagcatttg gctgggctgt
     7921 gggcacaccg acagaggaca gctctcattt cttgacttaa atactgaagg atacacttct
     7981 gaggaagttg ctgatagtag aatattgtgc ttagccttgg tgcatcttcc tgttgaaaag
     8041 gaaagctgga ttgtgtctgg gacacagtct ggtactctcc tggtcatcaa taccgaagat
     8101 gggaaaaaga gacataccct agaaaagatg actgattctg tcacttgttt gtattgcaat
     8161 tccttttcca agcaaagcaa acaaaaaaat tttcttttgg ttggaaccgc tgatggcaag
     8221 ttagcaattt ttgaagataa gactgttaag cttaaaggag ctgctccttt gaagatacta
     8281 aatataggaa atgtcagtac tccattgatg tgtttgagtg aatccacaaa ttcaacggaa
     8341 agaaatgtaa tgtggggagg atgtggcaca aagattttct ccttttctaa tgatttcacc
     8401 attcagaaac tcattgagac aagaacaagc caactgtttt cttatgcagc tttcagtgat
     8461 tccaacatca taacagtggt ggtagacact gctctctata ttgctaagca aaatagccct
     8521 gttgtggaag tgtgggataa gaaaactgaa aaactctgtg gactaataga ctgcgtgcac
     8581 tttttaaggg aggtaatggt aaaagaaaac aaggaatcaa aacacaaaat gtcttattct
     8641 gggagagtga aaaccctctg ccttcagaag aacactgctc tttggatagg aactggagga
     8701 ggccatattt tactcctgga tctttcaact cgtcgactta tacgtgtaat ttacaacttt
     8761 tgtaattcgg tcagagtcat gatgacagca cagctaggaa gccttaaaaa tgtcatgctg
     8821 gtattgggct acaaccggaa aaatactgaa ggtacacaaa agcagaaaga gatacaatct
     8881 tgcttgaccg tttgggacat caatcttcca catgaagtgc aaaatttaga aaaacacatt
     8941 gaagtgagaa aagaattagc tgaaaaaatg agacgaacat ctgttgagta agagagaagg
     9001 gcgaattcga cccagctttc ttgtacaaag tggttgatat cggtaccagt cgactctaga
     9061 ggatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     9121 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     9181 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     9241 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     9301 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     9361 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     9421 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     9481 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     9541 ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc
     9601 tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt
     9661 tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc tgtggaatgt
     9721 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9781 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9841 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9901 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9961 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10021 cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt
    10081 taattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt tcctgtgtga
    10141 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10201 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10261 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10321 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10381 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10441 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10501 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10561 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10621 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10681 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10741 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10801 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10861 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10921 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10981 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11041 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11101 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11161 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11221 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11281 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11341 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11401 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11461 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11521 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11581 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11641 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11701 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11761 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11821 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11881 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11941 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12001 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12061 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12121 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12181 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12241 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
    12301 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    12361 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    12421 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    12481 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    12541 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    12601 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    12661 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    12721 acaattt
//
