LOCUS       sequence-382191-       13612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 
            3xFLAG on the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Kluss JH, Beilina A, Williamson CD, Lewis PA, Cookson MR, 
            Bonet-Ponce L
  TITLE     Lysosomal positioning regulates Rab10 phosphorylation at LRRK2(+) 
            lysosomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2205492119. doi: 
            10.1073/pnas.2205492119. Epub 2022 Oct 18.
  PUBMED    36256825
REFERENCE   2  (bases 1 to 13612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2205492119. 
            doi"; pages: " 10.1073/pnas.2205492119. Epub 2022 Oct 18"
COMMENT     Sequence Label: EE-LRRK2
FEATURES             Location/Qualifiers
     source          1..13612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2152..2217
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    2254..2278
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9895..9919)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9953..10575
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(10152..10171)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(10599..10619)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10604..10933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10784..10919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10971..10990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10972..10990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11004..11020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11004..11020)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11017..11039)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11028..11044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11052..11058,11059..11076,11077..11082))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11097..11118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11235..11252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11406..11994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11486..11505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12165..12956,12957..13025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12788..12807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13026..13130)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13157..13585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13244..13263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13454..13475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gagagagccc cagactgggt ggacgctgag
      961 gaatgccacc gctgcagggt gcagttcggg gtgatgaccc gtaagcacca ctgccgggcg
     1021 tgtgggcaga tattctgtgg aaagtgttct tccaagtact ccaccatccc caagtttggc
     1081 atcgagaagg aggtgcgcgt gtgtgagccc tgctacgagc agctgaacag gaaagcggag
     1141 ggaaaggcca cttccaccac tgagctgccc cccgagtacc tgaccagccc cctgtctcag
     1201 cagtcccagc tgccccccaa gagggacgag acggccctgc aggaggagga ggagctgcag
     1261 ctggccctgg cgctgtcaca gtcagaggcg gaggagaagg agaggctgag acagaagtcc
     1321 acgtacactt cgtaccccaa ggcggagccc atgccctcgg cctcctcagc gccacctgcc
     1381 agcagcctgt actcttcacc tgtgaactcg tcggcgcctc tggctgagga catcgaccct
     1441 gagctcgcac ggtatctcaa ccggaactac tgggagaaga aacaggagga ggctcgcaag
     1501 agccccacgc catctgcgcc cgtgcccctg acagagccgg ctgcacagcc tggtgaagga
     1561 cacgcagccc ctaccaacgt ggtggagaac cctctcccgg agacagactc tcagcccatt
     1621 cctccatctg gtggcccctt tagtgagcca cagttccaca atggcgagtc tgaggagagc
     1681 cacgagcagt tcctgaaggc gctgcagaac gccgtcacca ccttcgtgaa ccgcatgaag
     1741 agtaatcaca tgcgaggccg cagcatcacc aatgactcgg ccgtgctctc actctttcag
     1801 tccatcaacg gcatgcaccc gcagctgctg gagctgctca accagctgga cgagcgcagg
     1861 ctgtactatg agggtctgca ggacaagctg gcacagatcc gcgatgcccg tggtgcgctg
     1921 agtgccctgc gcgaagagca ccgggagaag cttcgccgtg cagccgaaga ggcagagcgc
     1981 cagcgccaga tccagctggc ccagaagctg gagataatgc ggcagaagaa gcaggagtac
     2041 ctggaggtgc agaggcagct ggccatccag cgcctgcagg agcaggagaa ggagcggcag
     2101 atgcggctgg agcagcagaa gcagacggtc cagatgcgcg gaggcagcgg tgactacaaa
     2161 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctt
     2221 gcggccgcga attcatcgat agatctgata tcaacaagtt tgtacaaaaa agcaggctcc
     2281 gaattcgccc ttatggctag tggcagctgt caggggtgcg aagaggacga ggaaactctg
     2341 aagaagttga tagtcaggct gaacaatgtc caggaaggaa aacagataga aacgctggtc
     2401 caaatcctgg aggatctgct ggtgttcacg tactccgagc acgcctccaa gttatttcaa
     2461 ggcaaaaata tccatgtgcc tctgttgatc gtcttggact cctatatgag agtcgcgagt
     2521 gtgcagcagg tgggttggtc acttctgtgc aaattaatag aagtctgtcc aggtacaatg
     2581 caaagcttaa tgggacccca ggatgttgga aatgattggg aagtccttgg tgttcaccaa
     2641 ttgattctta aaatgctaac agttcataat gccagtgtaa acttgtcagt gattggactg
     2701 aagaccttag atctcctcct aacttcaggt aaaatcacct tgctgatatt ggatgaagaa
     2761 agtgatattt tcatgttaat ttttgatgcc atgcactcat ttccagccaa tgatgaagtc
     2821 cagaaacttg gatgcaaagc tttacatgtg ctgtttgaga gagtctcaga ggagcaactg
     2881 actgaatttg ttgagaacaa agattatatg atattgttaa gtgcgttaac aaattttaaa
     2941 gatgaagagg aaattgtgct tcatgtgctg cattgtttac attccctagc gattccttgc
     3001 aataatgtgg aagtcctcat gagtggcaat gtcaggtgtt ataatattgt ggtggaagct
     3061 atgaaagcat tccctatgag tgaaagaatt caagaagtga gttgctgttt gctccatagg
     3121 cttacattag gtaatttttt caatatcctg gtattaaacg aagtccatga gtttgtggtg
     3181 aaagctgtgc agcagtaccc agagaatgca gcattgcaga tctcagcgct cagctgtttg
     3241 gccctcctca ctgagactat tttcttaaat caagatttag aggaaaagaa tgagaatcaa
     3301 gagaatgatg atgaggggga agaagataaa ttgttttggc tggaagcctg ttacaaagca
     3361 ttaacgtggc atagaaagaa caagcacgtg caggaggccg catgctgggc actaaataat
     3421 ctccttatgt accaaaacag tttacatgag aagattggag atgaagatgg ccatttccca
     3481 gctcataggg aagtgatgct ctccatgctg atgcattctt catcaaagga agttttccag
     3541 gcatctgcga atgcattgtc aactctctta gaacaaaatg ttaatttcag aaaaatactg
     3601 ttatcaaaag gaatacacct gaatgttttg gagttaatgc agaagcatat acattctccc
     3661 gaagtggctg aaagtggctg taaaatgcta aatcatcttt ttgaaggaag caacacttcc
     3721 ctggatataa tggcagcagt ggtccccaaa atactaacag ttatgaaacg tcatgagaca
     3781 tcattaccag tgcagctgga ggcgcttcga gctattttac attttatagt gcctggcatg
     3841 ccagaagaat ccagggagga tacagaattt catcataagc taaatatggt taaaaaacag
     3901 tgtttcaaga atgatattca caaactggtc ctagcagctt tgaacaggtt cattggaaat
     3961 cctgggattc agaaatgtgg attaaaagta atttcttcta ttgtacattt tcctgatgca
     4021 ttagagatgt tatccctgga aggtgctatg gattcagtgc ttcacacact gcagatgtat
     4081 ccagatgacc aagaaattca gtgtctgggt ttaagtctta taggatactt gattacaaag
     4141 aagaatgtgt tcataggaac tggacatctg ctggcaaaaa ttctggtttc cagcttatac
     4201 cgatttaagg atgttgctga aatacagact aaaggatttc agacaatctt agcaatcctc
     4261 aaattgtcag catctttttc taagctgctg gtgcatcatt catttgactt agtaatattc
     4321 catcaaatgt cttccaatat catggaacaa aaggatcaac agtttctaaa cctctgttgc
     4381 aagtgttttg caaaagtagc tatggatgat tacttaaaaa atgtgatgct agagagagcg
     4441 tgtgatcaga ataacagcat catggttgaa tgcttgcttc tattgggagc agatgccaat
     4501 caagcaaagg agggatcttc tttaatttgt caggtatgtg agaaagagag cagtcccaaa
     4561 ttggtggaac tcttactgaa tagtggatct cgtgaacaag atgtacgaaa agcgttgacg
     4621 ataagcattg ggaaaggtga cagccagatc atcagcttgc tcttaaggag gctggccctg
     4681 gatgttgcca acaatagcat ttgccttgga ggattttgta taggaaaagt tgaaccttct
     4741 tggcttggtc ctttatttcc agataagact tctaatttaa ggaaacaaac aaatatagca
     4801 tctacactag caagaatggt gatcagatat cagatgaaaa gtgctgtgga agaaggaaca
     4861 gcctcaggca gcgatggaaa tttttctgaa gatgtgctgt ctaaatttga tgaatggacc
     4921 tttattcctg actcttctat ggacagtgtg tttgctcaaa gtgatgacct ggatagtgaa
     4981 ggaagtgaag gctcatttct tgtgaaaaag aaatctaatt caattagtgt aggagaattt
     5041 taccgagatg ccgtattaca gcgttgctca ccaaatttgc aaagacattc caattccttg
     5101 gggcccattt ttgatcatga agatttactg aagcgaaaaa gaaaaatatt atcttcagat
     5161 gattcactca ggtcatcaaa acttcaatcc catatgaggc attcagacag catttcttct
     5221 ctggcttctg agagagaata tattacatca ctagaccttt cagcaaatga actaagagat
     5281 attgatgccc taagccagaa atgctgtata agtgttcatt tggagcatct tgaaaagctg
     5341 gagcttcacc agaatgcact cacgagcttt ccacaacagc tatgtgaaac tctgaagagt
     5401 ttgacacatt tggacttgca cagtaataaa tttacatcat ttccttctta tttgttgaaa
     5461 atgagttgta ttgctaatct tgatgtctct cgaaatgaca ttggaccctc agtggtttta
     5521 gatcctacag tgaaatgtcc aactctgaaa cagtttaacc tgtcatataa ccagctgtct
     5581 tttgtacctg agaacctcac tgatgtggta gagaaactgg agcagctcat tttagaagga
     5641 aataaaatat cagggatatg ctcccccttg agactgaagg aactgaagat tttaaacctt
     5701 agtaagaacc acatttcatc cctatcagag aactttcttg aggcttgtcc taaagtggag
     5761 agtttcagtg ccagaatgaa ttttcttgct gctatgcctt tcttgcctcc ttctatgaca
     5821 atcctaaaat tatctcagaa caaattttcc tgtattccag aagcaatttt aaatcttcca
     5881 cacttgcggt ctttagatat gagcagcaat gatattcagt acctaccagg tcccgcacac
     5941 tggaaatctt tgaacttaag ggaactctta tttagccata atcagatcag catcttggac
     6001 ttgagtgaaa aagcatattt atggtctaga gtagagaaac tgcatctttc tcacaataaa
     6061 ctgaaagaga ttcctcctga gattggctgt cttgaaaatc tgacatctct ggatgtcagt
     6121 tacaacttgg aactaagatc ctttcccaat gaaatgggga aattaagcaa aatatgggat
     6181 cttcctttgg atgaactgca tcttaacttt gattttaaac atataggatg taaagccaaa
     6241 gacatcataa ggtttcttca acagcgatta aaaaaggctg tgccttataa ccgaatgaaa
     6301 cttatgattg tgggaaatac tgggagtggt aaaaccacct tattgcagca attaatgaaa
     6361 accaagaaat cagatcttgg aatgcaaagt gccacagttg gcatagatgt gaaagactgg
     6421 cctatccaaa taagagacaa aagaaagaga gatctcgtcc taaatgtgtg ggattttgca
     6481 ggtcgtgagg aattctatag tactcatccc cattttatga cgcagcgagc attgtacctt
     6541 gctgtctatg acctcagcaa gggacaggct gaagttgatg ccatgaagcc ttggctcttc
     6601 aatataaagg ctcgcgcttc ttcttcccct gtgattctcg ttggcacaca tttggatgtt
     6661 tctgatgaga agcaacgcaa agcctgcatg agtaaaatca ccaaggaact cctgaataag
     6721 cgagggttcc ctgccatacg agattaccac tttgtgaatg ccaccgagga atctgatgct
     6781 ttggcaaaac ttcggaaaac catcataaac gagagcctta atttcaagat ccgagatcag
     6841 cttgttgttg gacagctgat tccagactgc tatgtagaac ttgaaaaaat cattttatcg
     6901 gagcgtaaaa atgtgccaat tgaatttccc gtaattgacc ggaaacgatt attacaacta
     6961 gtgagagaaa atcagctgca gttagatgaa aatgagcttc ctcacgcagt tcactttcta
     7021 aatgaatcag gagtccttct tcattttcaa gacccagcac tgcagttaag tgacttgtac
     7081 tttgtggaac ccaagtggct ttgtaaaatc atggcacaga ttttgacagt gaaagtggaa
     7141 ggttgtccaa aacaccctaa gggaattatt tcgcgtagag atgtggaaaa atttctttca
     7201 aaaaaaagga aatttccaaa gaactacatg tcacagtatt ttaagctcct agaaaaattc
     7261 cagattgctt tgccaatagg agaagaatat ttgctggttc caagcagttt gtctgaccac
     7321 aggcctgtga tagagcttcc ccattgtgag aactctgaaa ttatcatccg actatatgaa
     7381 atgccttatt ttccaatggg attttggtca agattaatca atcgattact tgagatttca
     7441 ccttacatgc tttcagggag agaacgagca cttcgcccaa acagaatgta ttggcgacaa
     7501 ggcatttact taaattggtc tcctgaagct tattgtctgg taggatctga agtcttagac
     7561 aatcatccag agagtttctt aaaaattaca gttccttctt gtagaaaagg ctgtattctt
     7621 ttgggccaag ttgtggacca cattgattct ctcatggaag aatggtttcc tgggttgctg
     7681 gagattgata tttgtggtga aggagaaact ctgttgaaga aatgggcatt atatagtttt
     7741 aatgatggcg aagaacatca aaaaatctta cttgatgact tgatgaagaa agcagaggaa
     7801 ggagatctct tagtaaatcc agatcaacca aggctcacca ttccaatatc tcagattgcc
     7861 cctgacttga ttttggctga cctgcctaga aatattatgt tgaataatga tgagttggaa
     7921 tttgaacaag ctccagagtt tctcctaggt gatggcagtt ttggatcagt ttaccgagca
     7981 gcctatgaag gagaagaagt ggctgtgaag atttttaata aacatacatc actcaggctg
     8041 ttaagacaag agcttgtggt gctttgccac ctccaccacc ccagtttgat atctttgctg
     8101 gcagctggga ttcgtccccg gatgttggtg atggagttag cctccaaggg ttccttggat
     8161 cgcctgcttc agcaggacaa agccagcctc actagaaccc tacagcacag gatcgcactc
     8221 cacgtagctg atggtttgag atacctccac tcagccatga ttatataccg agacctgaaa
     8281 ccccacaatg tgctgctttt cacactgtat cccaatgctg ccatcatcgc aaagattgct
     8341 gactacggca ttgctcagta ctgctgtaga atggggataa aaacatcaga gggcacacca
     8401 gggtttcgtg cacctgaagt tgccagagga aatgtcattt ataaccaaca ggctgatgtt
     8461 tattcatttg gtttactact ctatgacatt ttgacaactg gaggtagaat agtagagggt
     8521 ttgaagtttc caaatgagtt tgatgaatta gaaatacaag gaaaattacc tgatccagtt
     8581 aaagaatatg gttgtgcccc atggcctatg gttgagaaat taattaaaca gtgtttgaaa
     8641 gaaaatcctc aagaaaggcc tacttctgcc caggtctttg acattttgaa ttcagctgaa
     8701 ttagtctgtc tgacgagacg cattttatta cctaaaaacg taattgttga atgcatggtt
     8761 gctacacatc acaacagcag gaatgcaagc atttggctgg gctgtgggca caccgacaga
     8821 ggacagctct catttcttga cttaaatact gaaggataca cttctgagga agttgctgat
     8881 agtagaatat tgtgcttagc cttggtgcat cttcctgttg aaaaggaaag ctggattgtg
     8941 tctgggacac agtctggtac tctcctggtc atcaataccg aagatgggaa aaagagacat
     9001 accctagaaa agatgactga ttctgtcact tgtttgtatt gcaattcctt ttccaagcaa
     9061 agcaaacaaa aaaattttct tttggttgga accgctgatg gcaagttagc aatttttgaa
     9121 gataagactg ttaagcttaa aggagctgct cctttgaaga tactaaatat aggaaatgtc
     9181 agtactccat tgatgtgttt gagtgaatcc acaaattcaa cggaaagaaa tgtaatgtgg
     9241 ggaggatgtg gcacaaagat tttctccttt tctaatgatt tcaccattca gaaactcatt
     9301 gagacaagaa caagccaact gttttcttat gcagctttca gtgattccaa catcataaca
     9361 gtggtggtag acactgctct ctatattgct aagcaaaata gccctgttgt ggaagtgtgg
     9421 gataagaaaa ctgaaaaact ctgtggacta atagactgcg tgcacttttt aagggaggta
     9481 atggtaaaag aaaacaagga atcaaaacac aaaatgtctt attctgggag agtgaaaacc
     9541 ctctgccttc agaagaacac tgctctttgg ataggaactg gaggaggcca tattttactc
     9601 ctggatcttt caactcgtcg acttatacgt gtaatttaca acttttgtaa ttcggtcaga
     9661 gtcatgatga cagcacagct aggaagcctt aaaaatgtca tgctggtatt gggctacaac
     9721 cggaaaaata ctgaaggtac acaaaagcag aaagagatac aatcttgctt gaccgtttgg
     9781 gacatcaatc ttccacatga agtgcaaaat ttagaaaaac acattgaagt gagaaaagaa
     9841 ttagctgaaa aaatgagacg aacatctgtt gagtaagaga gaagggcgaa ttcgacccag
     9901 ctttcttgta caaagtggtt gatatcggta ccagtcgact ctagaggatc ccgggtggca
     9961 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
    10021 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
    10081 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
    10141 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
    10201 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
    10261 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
    10321 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
    10381 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa
    10441 ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc
    10501 ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta
    10561 gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc agttagggtg
    10621 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10681 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10741 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10861 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10921 aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt ccctatagtg
    10981 agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
    11041 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    11101 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    11161 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    11221 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    11281 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    11341 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    11401 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    11461 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    11521 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    11581 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    11641 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    11701 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    11761 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11821 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    11881 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11941 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    12001 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    12061 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    12121 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    12181 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    12241 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    12301 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    12361 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    12421 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    12481 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    12541 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    12601 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    12661 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    12721 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    12781 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    12841 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12901 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    12961 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    13021 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    13081 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    13141 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    13201 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    13261 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    13321 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    13381 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    13441 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    13501 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    13561 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat tt
//
