LOCUS       sequence-382208-        9057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP 
            through P2A self-cleaving peptides..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Gu Q, Yang X, Lv J, Zhang J, Xia B, Kim JD, Wang R, Xiong F, Meng S,
            Clements TP, Tandon B, Wagner DS, Diaz MF, Wenzel PL, Miller YI, 
            Traver D, Cooke JP, Li W, Zon LI, Chen K, Bai Y, Fang L
  TITLE     AIBP-mediated cholesterol efflux instructs hematopoietic stem and 
            progenitor cell fate.
  JOURNAL   Science. 2019 Mar 8;363(6431):1085-1088. doi: 
            10.1126/science.aav1749. Epub 2019 Jan 31.
  PUBMED    30705153
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aav1749"; journalName: "Science"; date: "2019-03-8-
            8"; volume: "363"; issue: "6431"; pages: "1085-1088"
COMMENT     Sequence Label: pBT2-4xUAS:NICD-P2A-mRFP
FEATURES             Location/Qualifiers
     source          1..9057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(34..45,46..208)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1246..1262
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1247..1263
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1541..1550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3662..3718
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3725..4402
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             3725..4402
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(3855..3873)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4123..4142
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4317..4340
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4441..4575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4465..4484)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4519..4538
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6030..6217,6218..6229)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        complement(6247..6265)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6286..6302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6286..6302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6299..6321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6310..6326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6334..6340,6341..6358,6359..6364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6379..6400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6517..6534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6688..7276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6768..6787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7447..8238,8239..8307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8070..8089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8308..8412)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8438..8893)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8575..8596)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8787..8806
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9020..9042
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9034..9051
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9035..9051
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattggag ctccagaggt gtaaaaagta ctcaaaaatt
       61 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      121 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      181 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      241 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      301 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      361 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      421 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      481 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
      541 cagatcttac gaagttatag atcctctagc gataagcttg atatcgaatt cgagttggcg
      601 cgcctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg
      661 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
      721 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
      781 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
      841 ctgggggctt tgggaggggg ctgtccccgt gagctcccag gcgcgcctgt cattctaaat
      901 ctctctttca gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag
      961 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
     1021 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
     1081 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
     1141 gggctgtccc cgtgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     1201 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa acccctcgag gtcgacggta
     1261 tcgataagct tgatatcgaa ttcctgcagc ccgggggatc acgcggccat caagcttagg
     1321 cctccacggt ggcttctaat ccgtgagtcc tagcgggtga cagccctccg tcttcacagg
     1381 cggaggagag tcttccgtag ggttcctcgg agtactgtcc tccgacgcgt gcaagggtcg
     1441 actctagagg gtatataatg gatcccatcg cgtctcagcc tcactttgag ctcctccaca
     1501 cgaattccct cgacctcgaa gacgcgtgga tccaccggtc gccaccatgg tggtcgtctc
     1561 caggaagagg aagcgggaac atggccagct ttggttcccc gagggattta aagtcaacga
     1621 acccaaaaag aagaggagag aaccagtggg agaggactct gtcggactca agcctttgaa
     1681 aaactcagac agctctctga tggatgaaca gctgagcgaa tgggctgaag acgacaccaa
     1741 taaacgattc cggtttgagg ggcagagcat tctggagatg agcggtcagt tggatcaccg
     1801 tcagtggact cagcagcatc tggacgctgc agacctgcgc ttgaactcga tggctccgac
     1861 gccgccgcag gggcagatcg aaaacgactg catggacgtc aatgtccgcg ggcctgacgg
     1921 attcactcca ctgatgatcg catcgtgtag cggcggcggt cttgaaaatg aaaacggcga
     1981 agcagaagaa gatccgtccg ctgacgtgat cacagatttt atttatcacg gagccaacct
     2041 gcacaatcag acagaccgca caggcgagac cgcacttcat ctggcagcac gatacgctcg
     2101 ctcggatgct gctaaacgtc tgctggagtc gtgcgccgat gctaatgtgc aggataacat
     2161 gggccggaca ccgctgcacg ccgccgtcgc tgctgatgca cagggagtct tccagatcct
     2221 cattcggaac cgagcgacgg atctagacgc ccgcatgcat gatgggacga cgccgctgat
     2281 tctggccacc cgattggctg ttgagggaat ggtggaggag ctcattaact gccatgcaga
     2341 tccgaatgct gttgatgatt caggtaaatc agctcttcac tgggcggctg ctgttaataa
     2401 tgtggatgct gctgtcgtgc tgttgaagaa tggagctaat aaagatctgc agaataataa
     2461 ggaggaaacg cctcttttcc tcgctgctcg tgagggaagt tacgaaacgg ctaaagtcct
     2521 gcttgatcac ttggccaatc gggatattgc agatcacctg gaccagctgc cccgggacat
     2581 cgctcatgag cgcatgcacc acgatatcgt gcgtctgttg gaggaatata accttgtccg
     2641 aagccccccg ctacccttat cacccccgct ctgctgccca aacacatacc tgggcatcaa
     2701 acccagccca ggaaacaaca acaacacggc taagaaaaca cgcaaacccg gaggaaaagg
     2761 tgttggaggg aaagacagcg ggaaggacat cagaaccaaa aagaaaaaga gcggagatgg
     2821 caaaaacggc gggattatgg aagtgggcgt cctctcgcct gttgattcgc tggagtctcc
     2881 acacggctac ctatcagacg tctcgtctcc gcccatgatg acatcaccct tccagcagtc
     2941 tccgcccatc agtttaaatc agctccaggg attggccgac tcccacatgg gtggagctct
     3001 tcagggttta gggaagcctt ttgactccgc ccctcgttta tcccatctgc cggtggccaa
     3061 caatgtgggt ggagctcaag caggcgcttg tgattggctg caaagggtgc agcagcagca
     3121 gcaacaacaa caacaacaac agcaggcagg catgctaatg cctacaatgc tctctgctac
     3181 taatatgcca caagtcatgg gctatcccac catgcaaagc agccatcttg gtgcaccgtc
     3241 acacatgatc gcccatcaaa acatggcgcc aatgcagcac caaaacatct cgcatcactt
     3301 cctcggggac ctcagtgggc tggatcttca gtcaagctca ggacacgccc ccattcagac
     3361 catcctaccg caggacagcc agcgcatggc tccgcctatt tctagcacgc agtttctgac
     3421 tccgccttct cagcatagct attctaaccc tatggacaac acacccaatc accagcaggt
     3481 cccagaccac ccgttcctca caccctcggc cgggtctccc gaccaatggt ccagctcatc
     3541 tccacattcc aacttgtctg attggtcgga aggaatctcg agcccgccca ccagtatgca
     3601 gatgaaccat attccagaag ccttcaagac cggtggatcc ctcgagcccg ggtctagagg
     3661 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctag
     3721 atccatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
     3781 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3841 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     3901 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
     3961 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4021 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     4081 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     4141 gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg
     4201 cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
     4261 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
     4321 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
     4381 ccgccactcc accggcgcct aatcgattct agctagaact atagtgagtc gtattacgta
     4441 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4501 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4561 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4621 gtgtgggagg ttttttaatt cgcggccgcg gcgactagtg ggtttattgg atacacgggt
     4681 acccgggcga cgcagtctat cggaggactg gcgcgccgag tgaggggttg tgggctcttt
     4741 tattgagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     4801 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     4861 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     4921 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     4981 aaaagcttta ggctgaaaga gagatttaga atgacaggcg cgcctgggag ctcacgggga
     5041 cagccccctc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     5101 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     5161 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     5221 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa
     5281 agagagattt agaatgacag gcgcgcctgg gagctcacgg ggacagcccc ctcccaaagc
     5341 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
     5401 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
     5461 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
     5521 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
     5581 caggcgcgcc aactcgaatt cgatatcaag cttatcgcta gaggatctat aacttcgtag
     5641 ggcccgcggg agacagagga cacatttcat cttgaatgaa tctttattta caccacttaa
     5701 agaaaagaat tcgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac
     5761 tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac
     5821 tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca
     5881 tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg
     5941 attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg
     6001 gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga
     6061 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt
     6121 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat
     6181 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg gtaccagctt
     6241 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     6301 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6361 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8041 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8401 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     8461 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     8521 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     8581 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     8641 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     8701 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     8761 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     8821 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     8881 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8941 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     9001 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattg
//
