LOCUS       sequence-382231-        8659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of mouse Gata2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: FUW-Gata2
FEATURES             Location/Qualifiers
     source          1..8659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..42)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        214..593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        595..793
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     748..768
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             811..991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1038..1163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1656..1889
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2074..2118
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2416..2533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2598..3809
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3646..3665
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5301..5889
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5354..5374)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5772..5783)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5892..5908)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5893..5909)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6414..6594
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6620..6637)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6626..6737
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6883..7471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6963..6982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7642..8433,8434..8502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8265..8284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8503..8607)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
       61 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
      121 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      181 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      601 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      721 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      781 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      841 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      901 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      961 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     1021 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     1081 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1141 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1201 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1261 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1321 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1381 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1441 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1501 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1561 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1621 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1681 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1741 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1801 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1861 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1921 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1981 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     2041 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     2101 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     2161 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     2221 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2281 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2341 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     2401 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2461 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2521 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaaccc
     2581 gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
     2641 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
     2701 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
     2761 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
     2821 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
     2881 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
     2941 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
     3001 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
     3061 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
     3121 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
     3181 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
     3241 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     3301 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     3361 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     3421 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     3481 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     3541 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     3601 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     3661 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     3721 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     3781 tctggccgtt tttggctttt ttgttagacg aagcttgggc ccgggatcca tggaggtggc
     3841 gcctgagcag cctcgctgga tggcgcaccc cgccgtattg aatgcgcagc accccgactc
     3901 gcaccatccg ggcctggcgc ataactacat ggagccagca cagctgctgc ctcccgacga
     3961 ggtggatgtc ttcttcaacc atctcgactc gcagggcaac ccttactacg ccaacccggc
     4021 ccacgcgcgc gcgcgcgttt cctacagccc ggcgcatgcc cgtctcaccg gaggccagat
     4081 gtgccgacca cacttgttgc acagcccagg cttgccgtgg ctggacgggg gcaaagcagc
     4141 tctctctgcc gccgctgccc atcaccacag tccctggacc gtcagcccgt tctccaagac
     4201 cccgctgcac ccctcagctg ctggagcacc cggagggcct ctgtctgttt acccaggggc
     4261 tgcgggtggg agcgggggag gcagtgggag ctccgtggcc tccctcaccc ccactgcagc
     4321 ccactcgggc tcccatctct tcggcttccc acccacgcca cccaaagaag tgtctccaga
     4381 ccccagcaca acaggagctg cttccccggc ctcttcttct gcagggggta gtgtagcccg
     4441 gggtgaggac aaggatggcg tcaagtacca agtgtcactc tccgagagca tgaagatgga
     4501 aggcggcagt cccctgcgcc cgggcctagc taccatgggc acccagcctg caacacacca
     4561 cccgataccc acctatccct cctatgtgcc cgccgcagct catgactatg gcagcagtct
     4621 cttccatcca ggaggcttcc tgggtggccc cgcctccagc ttcaccccta agcagagaag
     4681 caaggctcgc tcctgctcag aaggccggga gtgtgtcaac tgtggtgcca cagccacccc
     4741 tctctggcga cgagatggca cgggccacta cctgtgcaat gcctgtgggc tctaccacaa
     4801 gatgaatgga cagaaccggc cgctcatcaa gcccaagcgg aggctgtctg ctgccagaag
     4861 agcgggcacc tgttgtgcaa attgtcagac gacaaccacc accttatggc gccggaacgc
     4921 caacggggac cctgtgtgca acgcctgtgg cctctactac aagctgcaca atgttaacag
     4981 gccactgacc atgaagaagg aagggatcca gacccggaat cggaagatgt ccagcaaatc
     5041 caagaaaagc aagaaagggg ctgaatgttt cgaggagctc tccaagtgca tgcaagagaa
     5101 gtcaccgccc ttcagtgcgg ctgccctggc tggacacatg gcacctgtgg gacacctccc
     5161 accttttagt cactctggac acatcctacc cacgcccacg cctatccacc cttcctccag
     5221 tctctctttt ggccaccccc acccgtccag catggtgact gccatgggct agggatccat
     5281 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5341 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5401 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5461 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5521 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5581 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5641 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5701 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5761 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5821 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5881 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5941 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6001 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6061 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6121 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6181 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6241 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6301 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6361 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6421 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6481 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6541 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6601 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6661 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6721 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6781 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgtgag caaaaggcca
     6841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     8641 cggatcggga gatctcccg
//
