LOCUS       sequence-382233-        8659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of mouse Gata2 with mutation C373R in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: FUW-Gata2-C373R
FEATURES             Location/Qualifiers
     source          1..8659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..41)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        213..592
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        594..792
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     747..767
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             810..990
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1037..1162
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1655..1888
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2073..2117
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2415..2532
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2597..3808
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3645..3664
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5300..5888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5353..5373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5771..5782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5891..5907)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5892..5908)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6413..6593
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6619..6636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6625..6736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6882..7470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6962..6981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7641..8432,8433..8501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8264..8283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8502..8606)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
       61 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
      121 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      181 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      241 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      301 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      361 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      421 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      481 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      541 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      601 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      661 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      721 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      781 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      961 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1021 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1081 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     1141 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     1201 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1261 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1321 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1381 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1441 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1501 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1561 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1621 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1681 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1741 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1801 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1861 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1921 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1981 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     2041 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     2101 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     2161 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     2221 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2281 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2341 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     2401 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     2461 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     2521 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt aattaacccg
     2581 tgtcggctcc agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     2641 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     2701 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     2761 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     2821 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     2881 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     2941 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     3001 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     3061 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     3121 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     3181 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     3241 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     3301 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     3361 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     3421 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     3481 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     3541 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     3601 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     3661 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     3721 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     3781 ctggccgttt ttggcttttt tgttagacga agcttgggcc cgggatccat ggaggtggcg
     3841 cctgagcagc ctcgctggat ggcgcacccc gccgtattga atgcgcagca ccccgactcg
     3901 caccatccgg gcctggcgca taactacatg gagccagcac agctgctgcc tcccgacgag
     3961 gtggatgtct tcttcaacca tctcgactcg cagggcaacc cttactacgc caacccggcc
     4021 cacgcgcgcg cgcgcgtttc ctacagcccg gcgcatgccc gtctcaccgg aggccagatg
     4081 tgccgaccac acttgttgca cagcccaggc ttgccgtggc tggacggggg caaagcagct
     4141 ctctctgccg ccgctgccca tcaccacagt ccctggaccg tcagcccgtt ctccaagacc
     4201 ccgctgcacc cctcagctgc tggagcaccc ggagggcctc tgtctgttta cccaggggct
     4261 gcgggtggga gcgggggagg cagtgggagc tccgtggcct ccctcacccc cactgcagcc
     4321 cactcgggct cccatctctt cggcttccca cccacgccac ccaaagaagt gtctccagac
     4381 cccagcacaa caggagctgc ttccccggcc tcttcttctg cagggggtag tgtagcccgg
     4441 ggtgaggaca aggatggcgt caagtaccaa gtgtcactct ccgagagcat gaagatggaa
     4501 ggcggcagtc ccctgcgccc gggcctagct accatgggca cccagcctgc aacacaccac
     4561 ccgataccca cctatccctc ctatgtgccc gccgcagctc atgactatgg cagcagtctc
     4621 ttccatccag gaggcttcct gggtggcccc gcctccagct tcacccctaa gcagagaagc
     4681 aaggctcgct cctgctcaga aggccgggag tgtgtcaact gtggtgccac agccacccct
     4741 ctctggcgac gagatggcac gggccactac ctgtgcaatg cctgtgggct ctaccacaag
     4801 atgaatggac agaaccggcc gctcatcaag cccaagcgga ggctgtctgc tgccagaaga
     4861 gcgggcacct gttgtgcaaa ttgtcagacg acaaccacca ccttatggcg ccggaacgcc
     4921 aacggggacc ctgtgtgcaa cgcccgtggc ctctactaca agctgcacaa tgttaacagg
     4981 ccactgacca tgaagaagga agggatccag acccggaatc ggaagatgtc cagcaaatcc
     5041 aagaaaagca agaaaggggc tgaatgtttc gaggagctct ccaagtgcat gcaagagaag
     5101 tcaccgccct tcagtgcggc tgccctggct ggacacatgg cacctgtggg acacctccca
     5161 ccttttagtc actctggaca catcctaccc acgcccacgc ctatccaccc ttcctccagt
     5221 ctctcttttg gccaccccca cccgtccagc atggtgactg ccatgggcta gggatccatt
     5281 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5341 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5401 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5461 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5521 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5581 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5641 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     5701 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5761 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5821 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5881 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     5941 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6001 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6061 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6121 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     6181 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     6241 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     6301 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     6361 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     6421 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6481 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6541 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     6601 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6661 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6721 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6781 ggcaggacag caagggggag gattgggaag acaatagcag gcatgtgagc aaaaggccag
     6841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     8641 ggatcgggag atctcccga
//
