LOCUS       sequence-382275-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N-terminally mGFP and C-terminally mScarlet labelled 
            wildtype FGFR3 (isoform IIIc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Hartl I, Brumovska V, Striedner Y, Yasari A, Schutz GJ, Sevcsik E, 
            Tiemann-Boege I
  TITLE     Measurement of FGFR3 signaling at the cell membrane via total 
            internal reflection fluorescence microscopy to compare the 
            activation of FGFR3 mutants.
  JOURNAL   J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832.
            Epub 2022 Dec 27.
  PUBMED    36581204
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-02"; volume: "299(2):102832. doi"; pages: " 
            10.1016/j.jbc.2022.102832. Epub 2022 Dec 27"
COMMENT     Sequence Label: pcDNA3.1_mGFP-FGFR3-mScarlet
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(995..997,998..1708)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1037..1058)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1298..1317)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1645..1666
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4124..4819
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4257..4275)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4525..4544
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4854..4871)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4860..5084
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5130..5558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5217..5236)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5427..5448
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5567..5587)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5572..5901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5752..5887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5814..5833
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5950..6975
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5950..6975
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7105..7226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7142..7161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7196..7215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7275..7291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7275..7291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7288..7310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7299..7315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7323..7329,7330..7347,7348..7353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7368..7389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7506..7523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7677..8265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7757..7776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8436..9227,9228..9296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9059..9078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9297..9401)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgggcgc ccctgcctgc gccctcgcgc tctgcgtggc
      961 cgtggccatc gtggccggcg cctcctcggg taccatgagc aagggcgagg agctgttcac
     1021 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1081 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1141 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1201 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1261 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1321 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1381 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1441 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1501 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1561 cgacggcccc gtgctgctgc ccgacaacca ctacctgagt acccagtcca aactgagcaa
     1621 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1681 cactctcggc atggacgagc tgtacaagcg tacgggtgga tcaggtggta gtggaggttc
     1741 tggtggttca ggtggatccg agtccttggg gacggagcag cgcgtcgtgg ggcgagcggc
     1801 agaagtcccg ggcccagagc ccggccagca ggagcagttg gtcttcggca gcggggatgc
     1861 tgtggagctg agctgtcccc cgcccggggg tggtcccatg gggcccactg tctgggtcaa
     1921 ggatggcaca gggctggtgc cctcggagcg tgtcctggtg gggccccagc ggctgcaggt
     1981 gctgaatgcc tcccacgagg actccggggc ctacagctgc cggcagcggc tcacgcagcg
     2041 cgtactgtgc cacttcagtg tgcgggtgac agacgctcca tcctcgggag atgacgaaga
     2101 cggggaggac gaggctgagg acacaggtgt ggacacaggg gccccttact ggacacggcc
     2161 cgagcggatg gacaagaagc tgctggccgt gccggccgcc aacaccgtcc gcttccgctg
     2221 cccagccgct ggcaacccca ctccctccat ctcctggctg aagaacggca gggagttccg
     2281 cggcgagcac cgcattggag gcatcaagct gcggcatcag cagtggagcc tggtcatgga
     2341 aagcgtggtg ccctcggacc gcggcaacta cacctgcgtc gtggagaaca agtttggcag
     2401 catccggcag acgtacacgc tggacgtgct ggagcgctcc ccgcaccggc ccatcctgca
     2461 ggcggggctg ccggccaacc agacggcggt gctgggcagc gacgtggagt tccactgcaa
     2521 ggtgtacagt gacgcacagc cccacatcca gtggctcaag cacgtggagg tgaatggcag
     2581 caaggtgggc ccggacggca caccctacgt taccgtgctc aagacggcgg gcgctaacac
     2641 caccgacaag gagctagagg ttctctcctt gcacaacgtc acctttgagg acgccgggga
     2701 gtacacctgc ctggcgggca attctattgg gttttctcat cactctgcgt ggctggtggt
     2761 gctgccagcc gaggaggagc tggtggaggc tgacgaggcg ggcagtgtgt atgcaggcat
     2821 cctcagctac ggggtgggct tcttcctgtt catcctggtg gtggcggctg tgacgctctg
     2881 ccgcctgcgc agccccccca agaaaggcct gggctccccc accgtgcaca agatctcccg
     2941 cttcccgctc aagcgacagg tgtccctgga gtccaacgcg tccatgagct ccaacacacc
     3001 actggtgcgc atcgcaaggc tgtcctcagg ggagggcccc acgctggcca atgtctccga
     3061 gctcgagctg cctgccgacc ccaaatggga gctgtctcgg gcccggctga ccctgggcaa
     3121 gccccttggg gagggctgct tcggccaggt ggtcatggcg gaggccatcg gcattgacaa
     3181 ggaccgggcc gccaagcctg tcaccgtagc cgtgaagatg ctgaaagacg atgccactga
     3241 caaggacctg tcggacctgg tgtctgagat ggagatgatg aagatgatcg ggaaacacaa
     3301 aaacatcatc aacctgctgg gcgcctgcac gcagggcggg cccctgtacg tgctggtgga
     3361 gtacgcggcc aagggtaacc tgcgggagtt tctgcgggcg cggcggcccc cgggcctgga
     3421 ctactccttc gacacctgca agccgcccga ggagcagctc accttcaagg acctggtgtc
     3481 ctgtgcctac caggtggccc ggggcatgga gtacttggcc tcccagaagt gcatccacag
     3541 ggacctggct gcccgcaatg tgctggtgac cgaggacaac gtgatgaaga tcgcagactt
     3601 cgggctggcc cgggacgtgc acaacctcga ctactacaag aagacaacca acggccggct
     3661 gcccgtgaag tggatggcgc ctgaggcctt gtttgaccga gtctacactc accagagtga
     3721 cgtctggtcc tttggggtcc tgctctggga gatcttcacg ctggggggct ccccgtaccc
     3781 cggcatccct gtggaggagc tcttcaagct gctgaaggag ggccaccgca tggacaagcc
     3841 cgccaactgc acacacgacc tgtacatgat catgcgggag tgctggcatg ccgcgccctc
     3901 ccagaggccc accttcaagc agctggtgga ggacctggac cgtgtcctta ccgtgacgtc
     3961 caccgacgag tacctggacc tgtcggcgcc tttcgagcag tactccccgg gtggccagga
     4021 cacccccagc tccagctcct caggggacga ctccgtgttt gcccacgacc tgctgccccc
     4081 ggccccaccc agcagtgggg gctcgcggac gaccggtgga agcatggtga gcaagggcga
     4141 ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca
     4201 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4261 gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt
     4321 catgtacggc tccagggcct tcatcaagca ccccgccgac atccccgact actataagca
     4381 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt
     4441 gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg
     4501 cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc
     4561 gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct
     4621 gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca aggccaagaa
     4681 gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa
     4741 cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg
     4801 catggacgag ctgtacaagt aatctagagg gcccgtttaa acccgctgat cagcctcgac
     4861 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4921 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4981 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5041 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5101 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5161 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5221 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5281 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5341 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5401 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5461 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5521 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5581 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5641 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5701 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5821 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5881 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5941 cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     6001 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     6061 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     6121 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     6181 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     6241 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     6301 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     6361 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     6421 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     6481 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     6541 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     6601 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     6661 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     6721 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     6781 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     6841 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     6901 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     6961 agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7021 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7081 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7201 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7261 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7321 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7381 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7441 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7501 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7561 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7621 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7681 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7741 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7801 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7861 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7921 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7981 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8041 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8101 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8161 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8221 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8281 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8341 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8401 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8461 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8521 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8581 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8641 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8701 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8761 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8821 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8881 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8941 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9001 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9061 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9121 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9181 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9241 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9301 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9361 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9421 ccacctgacg tc
//
