LOCUS       sequence-382285-        8727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N-terminally mGFP labelled mutated FGFR3 (isoform 
            IIIc)-K650Q mutation (associated with HCH; target mutation: 
            c.1948A>C; silent mutation added on purpose: c.1938C>T).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Hartl I, Brumovska V, Striedner Y, Yasari A, Schutz GJ, Sevcsik E, 
            Tiemann-Boege I
  TITLE     Measurement of FGFR3 signaling at the cell membrane via total 
            internal reflection fluorescence microscopy to compare the 
            activation of FGFR3 mutants.
  JOURNAL   J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832.
            Epub 2022 Dec 27.
  PUBMED    36581204
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-02"; volume: "299(2):102832. doi"; pages: " 
            10.1016/j.jbc.2022.102832. Epub 2022 Dec 27"
COMMENT     Sequence Label: pcDNA3.1_mGFP-FGFR3-K650Q
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(995..997,998..1708)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1037..1058)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1298..1317)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1645..1666
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4149..4166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4155..4379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4425..4853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4512..4531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4722..4743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4862..4882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4867..5196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5047..5182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5109..5128
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5245..6270
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5245..6270
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6400..6521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6437..6456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6491..6510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6570..6586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6570..6586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6583..6605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6594..6610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6618..6624,6625..6642,6643..6648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6663..6684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6801..6818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6972..7560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7052..7071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7731..8522,8523..8591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8354..8373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8592..8696)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgggcgc ccctgcctgc gccctcgcgc tctgcgtggc
      961 cgtggccatc gtggccggcg cctcctcggg taccatgagc aagggcgagg agctgttcac
     1021 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1081 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1141 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1201 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1261 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1321 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1381 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1441 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1501 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1561 cgacggcccc gtgctgctgc ccgacaacca ctacctgagt acccagtcca aactgagcaa
     1621 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1681 cactctcggc atggacgagc tgtacaagga attcggtgga tcaggtggta gtggaggttc
     1741 tggtggttca ggtggatccg agtccttggg gacggagcag cgcgtcgtgg ggcgagcggc
     1801 agaagtcccg ggcccagagc ccggccagca ggagcagttg gtcttcggca gcggggatgc
     1861 tgtggagctg agctgtcccc cgcccggggg tggtcccatg gggcccactg tctgggtcaa
     1921 ggatggcaca gggctggtgc cctcggagcg tgtcctggtg gggccccagc ggctgcaggt
     1981 gctgaatgcc tcccacgagg actccggggc ctacagctgc cggcagcggc tcacgcagcg
     2041 cgtactgtgc cacttcagtg tgcgggtgac agacgctcca tcctcgggag atgacgaaga
     2101 cggggaggac gaggctgagg acacaggtgt ggacacaggg gccccttact ggacacggcc
     2161 cgagcggatg gacaagaagc tgctggccgt gccggccgcc aacaccgtcc gcttccgctg
     2221 cccagccgct ggcaacccca ctccctccat ctcctggctg aagaacggca gggagttccg
     2281 cggcgagcac cgcattggag gcatcaagct gcggcatcag cagtggagcc tggtcatgga
     2341 aagcgtggtg ccctcggacc gcggcaacta cacctgcgtc gtggagaaca agtttggcag
     2401 catccggcag acgtacacgc tggacgtgct ggagcgctcc ccgcaccggc ccatcctgca
     2461 ggcggggctg ccggccaacc agacggcggt gctgggcagc gacgtggagt tccactgcaa
     2521 ggtgtacagt gacgcacagc cccacatcca gtggctcaag cacgtggagg tgaatggcag
     2581 caaggtgggc ccggacggca caccctacgt taccgtgctc aagacggcgg gcgctaacac
     2641 caccgacaag gagctagagg ttctctcctt gcacaacgtc acctttgagg acgccgggga
     2701 gtacacctgc ctggcgggca attctattgg gttttctcat cactctgcgt ggctggtggt
     2761 gctgccagcc gaggaggagc tggtggaggc tgacgaggcg ggcagtgtgt atgcaggcat
     2821 cctcagctac ggggtgggct tcttcctgtt catcctggtg gtggcggctg tgacgctctg
     2881 ccgcctgcgc agccccccca agaaaggcct gggctccccc accgtgcaca agatctcccg
     2941 cttcccgctc aagcgacagg tgtccctgga gtccaacgcg tccatgagct ccaacacacc
     3001 actggtgcgc atcgcaaggc tgtcctcagg ggagggcccc acgctggcca atgtctccga
     3061 gctcgagctg cctgccgacc ccaaatggga gctgtctcgg gcccggctga ccctgggcaa
     3121 gccccttggg gagggctgct tcggccaggt ggtcatggcg gaggccatcg gcattgacaa
     3181 ggaccgggcc gccaagcctg tcaccgtagc cgtgaagatg ctgaaagacg atgccactga
     3241 caaggacctg tcggacctgg tgtctgagat ggagatgatg aagatgatcg ggaaacacaa
     3301 aaacatcatc aacctgctgg gcgcctgcac gcagggcggg cccctgtacg tgctggtgga
     3361 gtacgcggcc aagggtaacc tgcgggagtt tctgcgggcg cggcggcccc cgggcctgga
     3421 ctactccttc gacacctgca agccgcccga ggagcagctc accttcaagg acctggtgtc
     3481 ctgtgcctac caggtggccc ggggcatgga gtacttggcc tcccagaagt gcatccacag
     3541 ggacctggct gcccgcaatg tgctggtgac cgaggacaac gtgatgaaga tcgcagactt
     3601 cgggctggcc cgggacgtgc acaacctcga ttactacaag cagacaacca acggccggct
     3661 gcccgtgaag tggatggcgc ctgaggcctt gtttgaccga gtctacactc accagagtga
     3721 cgtctggtcc tttggggtcc tgctctggga gatcttcacg ctggggggct ccccgtaccc
     3781 cggcatccct gtggaggagc tcttcaagct gctgaaggag ggccaccgca tggacaagcc
     3841 cgccaactgc acacacgacc tgtacatgat catgcgggag tgctggcatg ccgcgccctc
     3901 ccagaggccc accttcaagc agctggtgga ggacctggac cgtgtcctta ccgtgacgtc
     3961 caccgacgag tacctggacc tgtcggcgcc tttcgagcag tactccccgg gtggccagga
     4021 cacccccagc tccagctcct caggggacga ctccgtgttt gcccacgacc tgctgccccc
     4081 ggccccaccc agcagtgggg gctcgcggac gtgataatct agagggcccg tttaaacccg
     4141 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4201 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4261 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4321 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4381 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4441 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4501 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4561 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4621 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4681 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4741 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4801 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     4861 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4921 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4981 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5041 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5101 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     5161 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     5221 attttcggat ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     5281 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     5341 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     5401 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     5461 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     5521 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     5581 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     5641 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     5701 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     5761 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     5821 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     5881 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     5941 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     6001 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     6061 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     6121 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     6181 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     6241 cccagcactc gtccgagggc aaaggaatag cacgtgctac gagatttcga ttccaccgcc
     6301 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6361 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6421 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6481 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6541 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6601 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6661 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6721 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6781 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6901 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6961 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7021 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7081 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7141 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7201 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7261 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7321 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7381 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7441 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7501 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7561 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7621 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7681 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7741 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7801 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7861 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7921 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7981 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8041 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8101 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8161 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8221 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8281 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8341 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8401 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8461 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8521 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8581 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8641 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8701 tttccccgaa aagtgccacc tgacgtc
//
