LOCUS       sequence-382316-       11668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  glr-4 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY91
FEATURES             Location/Qualifiers
     source          1..11668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             4728..4736
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5709..5726
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6997..7017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7039..7059
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9259..9281)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9381..9400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9478..9496)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9564..9668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9669..9737,9738..10529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9887..9906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10700..11288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11189..11208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11442..11459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11576..11597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11612..11617,11618..11635,11636..11642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11650..11666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(11655..11668,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tcacattcgc
      181 acactctact aaaaaagatt caataattca ctcaacaaca gattcatatc tgtttgaata
      241 ttatgatgaa tttatattta gtttgaaatg cacaagatta tgacaaaaaa gccacagtta
      301 agtgtttcgg cattcagtgg aaatttgtga gaaatttggt atttgctatt aggaaagttg
      361 aaaacaaaag attgcaaatt aatttcaatc gtaatacgcc aaatagcaaa ctcttgaaac
      421 ttaaaacgaa aattataaac aaatgtcaag aggtgttatg aagttgttta cagaaattat
      481 aaatttatat gagcacatgc catgtagtta aacatttgca agaaatagta atagtcttct
      541 gagaagctca tttttgaaat tgtaaatact atttgatccc gtgactcatt cttaccattg
      601 agtttaataa tgcatttttg tgattgttta atcataattg aattcctaac tcccatcagt
      661 ccgatctttc ctccgtctac tttatattta cttcagttct tttgttagag ggattgtctg
      721 ttaaaatatt taaaatcgaa attcttaaac tagtgaaact ccaacatgaa gacaggaata
      781 caaaagtatt ttaaaatggg tgtcaagttg acatctcgat tacggtaatt aacccatcca
      841 aatgttcacc agcaccacaa ttttatacaa gaattcgctt tccaattcgt aacaattaac
      901 ttgtctgtac aacagctccc gaagtgacac atgtgtcaac agtcacacaa tcacattatc
      961 catttctttc gttttttcgt tttattttgt ttgcagtgaa cgtcgttctt ttcattctag
     1021 tgaatggaat actgtagtta tgtaaatgga gtgtttgctc aattcatggg aaaatttaca
     1081 ttttattggg aacattgatg acaataaaat tacaatctcg gaatggaaag gaatgaatca
     1141 aattatacat aaagaaaggg acttacaaaa acttacaaaa gcatattaca gtacctcact
     1201 atatctcatc tttttacttt tccaaaaacc aacttttcac gcaattagat cttgtaaaat
     1261 gtcggtaact tcaaaaaaat gcggcaaatc ggcgcccttg acacgcggtt ttgccatcga
     1321 attttagaat ctgtgaaaaa catgacatga ggtaagacaa gattatgatc atttaggcca
     1381 tgtcacttcg aataacttta cagaagtgtc atgaaaaggt gatgaaagta accgcaggga
     1441 ttgaaggaaa atgaaaaaac gagtgaagtt cctggagaga aacattttca gatatcacat
     1501 aataaatgac aataatcagt ggctcgaaaa tcattgccaa ctcattctca ttgcctatta
     1561 ttttaatttt agtccctagt tccgttgttt tattcaaacc ttcagcaatt ccatccactg
     1621 tattttggga actagatgaa ttcacagaaa ttttttaaat ttggaagcac accaaaaaaa
     1681 attctaaaac tatttcaatt attttttata gttccactga acaaaacaat tctttaaaaa
     1741 tttcaagaat acaaaaaaag ttgtgttgaa aaacatatga ctcgaaaatc gctgaagtga
     1801 gaacaaaatt tgttaacttc taagtgttta aaaatgcctc gtgtcatttc atagaaaaca
     1861 accttttcga atttttactt tcagagttat agatcgttta ctggattttt tttaattttt
     1921 cttataaatc cttcaaactc aacacgtcaa tatcattttc ttcattttca gactattcta
     1981 ttttttgtgt tagttttata acacaaaaag gcacacttga gctgattgac tcacattcat
     2041 caactgaaat atttcattca ttagatctaa ttgggttttg ttttccagag actggaatct
     2101 aaaaacgaat tagttggaaa gattcgccca caatttgggt ttagtagttt acattttacg
     2161 ttaaaggtca gattgaatca tgacatttta ctggaaaaat ccacgtgatt ctgttttaaa
     2221 gatgttgagc cgggaagatt ggaagtttgg aagacaaaaa agattctgac aaaagttcac
     2281 cgaatctcaa tatttataat tcattacgtc ctactctttt ttgtttttcc ttccaaattt
     2341 ctactcatct gaaaccaaaa aatcttggag caaagacctt ccttcctcat ttttccccat
     2401 tcattttctc acctttttta ttgtttcggg tatgcataac aaataggtcg aaagacactt
     2461 cttccttttt cttctttcca gccgggcgga cggagcacaa acgagaagac cttcgtcgtt
     2521 gtcgtcggct tctttttttg ctcatttgat ttgtagcttt tcaagtggct tttttttgaa
     2581 accaaaattg ttttcgtttt gacaactaaa ctaaaaaact agcgtttcct ccctgtttcc
     2641 caacatttct tactcacaaa aataacataa taaatatcaa gtgaagttac tgctccagtt
     2701 tgattccaac tttgtaaaaa cactttttga aactttcagt gttttataat ataactcaaa
     2761 attcttcgat tactctaatt catctcacca agtttgtttc tgaaaatcgc acttacaatt
     2821 tctttgtctg aaagataata atcactacgg ggactctccc aatgcgaatt gtccctgtag
     2881 gcagatcgaa aggctcagta atctttcttc aaaaatgcac tctatttcat ttccttgtgt
     2941 tttactcgtt ttgtcatatt cacaatcaat tattcattta caaaagtcta taaaagcatg
     3001 taccaaaaaa gcacctgact ttttgtttcc atattccact ccaagttttt gaatttttca
     3061 tatttttgta tctaaattgt gttatagtct agtgagaaag ctaaaaagca tttcgaagta
     3121 aaagttaaaa acgcgctctc agagggtgca tgtttatctc ctttgaatcg ctttattggt
     3181 acttattcga atgtttttcg catgttgact accagaaaaa tggactcctg agagaattct
     3241 atgctcatcc gataccttcg gaacaaaggt atgatcttca aattaaatta atttttagtg
     3301 tttattacag tgatgattac agatacaaac attaatccca ataaaactta gtgaattttt
     3361 gaagttagta gttttaatct tttatttata tattcctacg tatcagttaa cgaaacaaaa
     3421 ttttcacatt ttttaattac atacataagt ccaacctttg ttacatactt tagaatgaaa
     3481 aaaattacag tttgataaat attttaagtt ttgtgttgct tgcaaaacat taaatgcaca
     3541 gcaaactctt gtaatcccac gtgctttctc acactttgct gctagaaatc ataccacatc
     3601 cttcaatgtc ccgaatctca aacaaatgtt tagtggcaat atgtcacact tttctttcgg
     3661 aacaccacct cccatagcat ttttcttgaa cattagtatt cccaacggat cttcgtgtgt
     3721 aggatgaaag tgcacggaga acaatttttc cgttttaaaa attgaatttt ccgcccttcc
     3781 ctgcaatatt ttgaactttc atcagactcc tactttagaa aaaatacgta ttttttctaa
     3841 aattactcaa tattaacagg gaagtcattc atctttcttg atctttctca cttgatcttc
     3901 ctgtcttgac ctttccatcc gagatttttc aaagttaaaa tcctcctccg cctgctcttt
     3961 cttttttttc gtttctttct tactcttcat ctttcatttt cttcataacc acttcgcact
     4021 ctttttctct tcacctcgat ctacgtttcg acatcttctg ctgccgtaca tttgcataga
     4081 attaaacgga ctttcgacga caagaggaag aagatgggag gaggagcaaa aagaaattcg
     4141 gagggagcaa cacagcttct tctgttaaca aacgggatta gttttgagta tgtggcaaag
     4201 gttactattc caataacttt ttgacttgtt caacttttct gccaagacct ttaaaaattc
     4261 tgtagattta cggtaataat ctagaaattc aaataatcat gttatgtgta caagattaat
     4321 ttaatttcta ttttcaacta ggaataattt ctataatttc agagaaaatt caaagactca
     4381 gccacgataa ttttattttt ttaattttaa ttttttgtac ttttagtctc atgaaaacta
     4441 tatttttgaa aactataatc agataatctc tgatttacta taaattcgag gatacttcaa
     4501 ttagctcata gatcaagaga agtagccagt tcttcaggat tggccgaaga aaaggaagat
     4561 tttgaacttt gaagtgagaa aagaggatga aaagaatgag gaacttggga cttttttttt
     4621 gtttttggag tgcaaaaatc gcgttgagca tgaattttga agaaaaaatt attcaaccac
     4681 atcattgatt tacctttgtt ctagtttttg aatttaaaaa aaattattaa ggaggttata
     4741 taacgtattg atgcttatac tgtatatact atactatata ctgtaacaat tggaagttaa
     4801 tttttctact caaaattgtt aggaaatttt ctgtaaattt tataaacgta tttttccgat
     4861 taaaaaatat tagccatcat tttgtaatcg aattcctgga aaaacctaga gcaactttct
     4921 gcctaagctg cgaatttgtt ttttgtagta tcatactttt tttaaatgtt ctaccctata
     4981 aaatagagta aatttcgaaa agaaacgttt aaatctagag aaaactcgtt ccttcctcta
     5041 ttaggttctt gtcaaatgtt ccatcttcct attttccaca ttttccaggg aatgattgac
     5101 aagatgtttg aatatgattc gagtgaaacc ccatagacac tttcagatct ttgctcacgt
     5161 tttccattga tttcatcaga atctagcaga caaatgaatg tgaagttcat tccattttct
     5221 gtttttgaaa tcaatgtcct aatatacttg tttgtcaaat agagcttgtc atttacactc
     5281 tacatatgtt tccttttcag gtttttgagc gaaattttac accgatcggt ttagaataat
     5341 atgcctaaaa aatggaaatt gtctgtttta aaaaactgac tggttaggag tttacgatag
     5401 cttctaaact atacaacttc aataatttgt gtccagactc caacaaaaaa ctataaaata
     5461 ttctagattg tgtctgcact acaaaatcaa agaaccgcct gaattcatat tatttccagt
     5521 taaattaatt cgtaactcca gtgaccttcg tcacttgaat atttttgagc gtcgccgcca
     5581 gaagattttt cttcagagct ctgagatttt tgaatgaaac tctgacacat gtcgttttct
     5641 cttgtctaca tatactctta ccagttcgac acacaattca atttcagagt ctcttttccg
     5701 ccccctatca tcatcatcat catcattctc gttttgtgct cgttggagcc gcccattttc
     5761 tctctctatc cttttttgtg tcccttttcc ttatttccat tcaaaaaaag tttgtcatga
     5821 tagtgcactt ttcagtagag agttgagaca gtctcattct gtcccccttc tgaatgtgat
     5881 catagacatc aaaaattagt tattctccga acgaaagacc cttttcggtt tttttctatc
     5941 atctctaata ttttgatcct ttcatcatga acttcaacgt ttcttctcca aaatagaaca
     6001 tgcatttaag ttccaaagaa accgaaaaat atagggaaaa gctgtgaaat tttaatagtt
     6061 ttcaaaacaa aaaagccaac tgcttctctc tcccaatccg gatttccctt atttttctca
     6121 aaaaaaggtc cagacaggca cagcttcaac cagtgctttt tgcagttgtt cttctaaatt
     6181 cattcacaca tttcaattta ttgaaacctt tcagtcaatt aatcttttat acaaacggta
     6241 gttacttgtt aaaaatcaga aatattgatt tttaaagctt catatccatt ttaaattgta
     6301 ataattacca gattacattg aagaattaat ttttgaaatt ttgtttcgaa atcgaacgaa
     6361 gtcgaggagt aacatttttg acaattcgac agttcgccga atccgtgctc aatttggttt
     6421 tcttgatttt ctaaatttcc tactgttcta ctgaaaatca aattttagaa aaaaagaaag
     6481 ttgagtctta agctcgtttt cctttttttt ttaacatgta actttttttt acagcaactg
     6541 gcaaaaagtt caaactcgtt tacgaaaaaa ttacatataa tgtggcaaaa aaagaagtcc
     6601 aaagttccgg aaaatcagat gaaatctgtg gtgcaatcaa actgcaattc actcctctca
     6661 taacctctct atttcgtttt cctctttctg atcttccctt gggagttcat tccttttctc
     6721 ctttcttttc cttttctctc ttcatttttt cgatagactc tttgatataa aattctgtgc
     6781 tgcaatccct tcctcacatt tttgtctatt cgtcatcgca tcgtttctca ctttttgtat
     6841 aattaatttt tatgatttta gatgagctaa acttttacac agcccgggat tggccaaagg
     6901 acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt
     6961 gcttggaggg taccgagctc agaaaaaatg accgctccaa agaagaagcg caaggttcca
     7021 gtcgagaaga tgactgcccc aaaaaagaaa cgtaaggtcc cgcgggtaag tttaaacagt
     7081 tcggtactaa ctaaccatac atatttaaat tttcagcgcg ctgtctccaa gggagaggag
     7141 ctcatcaagg agaacatgca caccaagctc tacatggagg gaaccgtcaa caaccaccac
     7201 ttcaagtgca cccacgaagg ggagggcaag ccctacgagg gcacccagac caacaggatt
     7261 aaggtggtgg agggaggccc cctgccgttc gcattcgaca tcctggccac ctgctttatg
     7321 tacgggagca agaccttcat caaccacacc caaggaatcc cagacttctt caagcaatcc
     7381 ttcgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagcctgag
     7441 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag
     7501 gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc
     7561 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc
     7621 ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc
     7681 gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac
     7741 ctcaagatgc caggagtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     7801 tttcaggtct actttgtgga ccgcagactg gaaagaatca aggaggccga caatgagacc
     7861 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     7921 cacaaactta atggcatgga cgagctgtac aagcctagga taccggtggg tgaagaccca
     7981 ccaaagccat ctgccaaggg agccaagaag gccgccaaga ccgttacgaa gccaaaggac
     8041 ggaaagaaga gacgtcatgc ccgtaaggaa tcatactccg tctacatcta ccgtgtcctc
     8101 aagcaagttc atccagacac tggagtttcc tccaaagcca tgtctatcat gaactctttt
     8161 gtcaacgatg tcttcgagcg tattgctgct gaagcatccc gtcttgctca ctacaacaag
     8221 cgttccacaa tctcatcccg cgaaattcag accgctgtcc gtctgatcct tccaggagag
     8281 cttgccaagc acgccgtgtc tgagggaacc aaggccgtta ccaagtacac ttccagcaag
     8341 taggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     8401 taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt
     8461 taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     8521 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
     8581 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     8641 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     8701 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     8761 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     8821 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     8881 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     8941 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt
     9001 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     9061 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     9121 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     9181 ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     9241 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     9301 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     9361 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9421 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc
     9481 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9661 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9721 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9781 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9841 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9901 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9961 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10021 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10081 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10141 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10201 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10261 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10321 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10381 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10441 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10501 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10561 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10621 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11401 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11461 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11521 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11581 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11641 tgtgtggaat tgtgagcgga taacaatt
//
