LOCUS       sequence-382324-        5388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY46
FEATURES             Location/Qualifiers
     source          1..5388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1063..1083
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1108..1128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1150..1170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2979..3001)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3101..3120
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3198..3216)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3284..3388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3389..3457,3458..4249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3607..3626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4420..5008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4909..4928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5296..5317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5332..5337,5338..5355,5356..5362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5370..5386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5375..5388,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg agtcatcatt
      181 ggagctgaac gtccaaagaa ataaatgaat ttgtttattg aataaatact attgagcatc
      241 gaatatttgt tctgaaaaga tcacatcgta aatttaaaac actgttttct cactttttaa
      301 tggaaaatat tcaattcttt gaaacttcgt ggaagctgaa aagtatcaga acacaaactc
      361 taaattttcc tgggggcttc tcccgcaact atgaaaatat aaatttccat aacttgttca
      421 ttccaaacct tatttcatca aattcagtca aaagaggttc cttttcatcc gctcgtccgt
      481 gtcccgaaaa tgaagatgtg ctgccctccc atccaagtgt ctctccctgt gtatgggtgg
      541 gggacgttga ttggccattt ctgtctacgt ctcttcttct catacactat tcaaatgctc
      601 agaggtgagt ctgaaatgca atttagggtt tgcgagtgct cggctccctc cgctcctact
      661 ctttacacac ttggctgcgg gcggacgcga aggtcctcca atttttcttc gtctcggtca
      721 cttcacttcg tactttttca atttcgccga aataagacta ttcttcctta gctgacttct
      781 ttcttgtact ctcacccttt ctaagacatg tgttaatccg aaagaacatc caattttctg
      841 tgtacaatga agtgaaggat caaatttccg agaagaaagg caaagcaagt cgacgtcgcc
      901 ggcacccaat ctgacacatt cacacccggg attggccaaa ggacccaaag gtatgtttcg
      961 aatgatacta acataacata gaacattttc aggaggaccc ttggagggta ccggttttcc
     1021 cagtcacgac gcgtggtacc gagctcagaa aaaatgaccg ctccaaagaa gaaacgcaaa
     1081 gtaccgagct cagaaaaaat gacggcacct aaaaagaaac gcaaagtacc ggtcgccgag
     1141 aagacagccc caaaaaagaa gcgtaaagtt ccacgcgtca tgggagtaag tttaaacagt
     1201 tcggtactaa ctaaccatac atatttaaat tttcagcgcg ctgtctccaa gggagaggag
     1261 ctcatcaagg agaacatgca caccaagctc tacatggagg gaaccgtcaa caaccaccac
     1321 ttcaagtgca cccacgaagg ggagggcaag ccctacgagg gcacccagac caacaggatt
     1381 aaggtggtgg agggaggccc cctgccgttc gcattcgaca tcctggccac ctgctttatg
     1441 tacgggagca agaccttcat caaccacacc caaggaatcc cagacttctt caagcaatcc
     1501 ttcgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagcctgag
     1561 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag
     1621 gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc
     1681 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc
     1741 ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc
     1801 gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac
     1861 ctcaagatgc caggagtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     1921 tttcaggtct actttgtgga ccgcagactg gaaagaatca aggaggccga caatgagacc
     1981 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     2041 cacaaactta atggcatgga cgagctgtac aagtaagcgc cggtcgctac cattaccaac
     2101 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     2161 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     2221 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     2281 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     2341 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     2401 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     2461 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     2521 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     2581 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     2641 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     2701 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     2761 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     2821 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     2881 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     2941 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3001 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3061 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     3121 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     3181 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3241 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3361 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3901 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4561 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5041 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5161 cagcgagtca gtgagcgatg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5221 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     5281 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     5341 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
