LOCUS       sequence-382362-        7959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chromogenic reporter for gene knockout in Streptomyces.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Dong J, Wei J, Li H, Zhao S, Guan W
  TITLE     An Efficient Markerless Deletion System Suitable for the Industrial 
            Strains of Streptomyces.
  JOURNAL   J Microbiol Biotechnol. 2021 Dec 28;31(12):1722-1731. doi: 
            10.4014/jmb.2106.06083.
  PUBMED    34489377
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4014/jmb.2106"; 
            journalName: "J Microbiol Biotechnol"; date: "2021-12-28- 28"; 
            volume: "31"; issue: "12"; pages: "1722-1731"
COMMENT     Sequence Label: pSUC01
FEATURES             Location/Qualifiers
     source          1..7959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             638..1438
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(1872..2243)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2276..2385
                     /note="incP origin of transfer"
     CDS             complement(2623..2634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2705..2804
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(7403..7419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7403..7419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7416..7438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7427..7443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7451..7457,7458..7475,7476..7481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7496..7517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7634..7651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(7805..7959,1..434))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7885..7904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
       61 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      541 ttttggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta
      601 tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa
      661 tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca accttggggt
      721 tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc ccctcgaaga
      781 tgggccactt ggactgatcg aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt
      841 catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt cgcccgttac
      901 accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc
      961 ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct ctgatccatt
     1021 gcccctgcca cctcactcgc ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca
     1081 ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg ccgagttgat
     1141 ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg gcaagttggt
     1201 acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg cggacaggtg
     1261 gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg ctcggttgat
     1321 ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc cgttgatctt
     1381 cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt cgattggctg
     1441 agctcatgag cggggaacga gatgacgttg gaggggcaag gtcgcgctga ttgctggggc
     1501 aacacgtgga gcggatcggg gattgtcttt cttcagctcg ctgatgatat gctgacgctc
     1561 aatgccgttt ggcctccgac taacgaaaat cccgcatttg gacggctgat ccgattggca
     1621 cggcggacgg cgaatggcgg agcagacgct cgtccggggg caatgagata tgaaaaagcc
     1681 tgaactcacc gcgacgtatc gggccctggc cagctagcta gagtcgacct gcaggtcccc
     1741 ggggatcggt cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct
     1801 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc
     1861 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc
     1921 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc
     1981 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg
     2041 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag
     2101 ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc
     2161 tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg
     2221 gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc
     2281 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
     2341 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
     2401 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
     2461 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag
     2521 atccgtcgac ctgcaggcat gcaagctcta gcgattccag acgtcccgaa ggcgtggcgc
     2581 ggcttccccg tgccggagca atcgccctgg gtgggttaca cgacgcccct ctatggcccg
     2641 tactgacgga cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg
     2701 ttttcccagg tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt
     2761 ctctcagttg ggggcgtagg gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca
     2821 cgtacgcggg tgcttacgac cgtcagtcgc gcgagcgcga aaattcgagc gcagcaagcc
     2881 cagcgacaca gcgtagcgcc aacgaagaca aggcggccga ccttcagcgc gaagtcgagc
     2941 gcgacggggg ccggttcagg ttcgtcgggc atttcagcga agcgccgggc acgtcggcgt
     3001 tcgggacggc ggagcgcccg gagttcgaac gcatcctgaa cgaatgccgc gccgggcggc
     3061 tcaacatgat cattgtctat gacgtgtcgc gcttctcgcg cctgaaggtc atggacgcga
     3121 ttccgattgt ctcggaattg ctcgccctgg gcgtgacgat tgtttccact caggaaggcg
     3181 tcttccggca gggaaacgtc atggacctga ttcacctgat tatgcggctc gacgcgtcgc
     3241 acaaagaatc ttcgctgaag tcggcgaaga ttctcgacac gaagaacctt cagcgcgaat
     3301 tgggcgggta cgtcggcggg aaggcgcctt acggcttcga gcttgtttcg gagacgaagg
     3361 agatcacgcg caacggccga atggtcaatg tcgtcatcaa caagctttgt tcacattcga
     3421 acggtctctg ctttgacaac atgctgtgcg gtgttgtaaa gtcgtggcca ggagaatacg
     3481 acagcgtgca ggactggggg agttatgagc accagcacgc cgccacccgc cacccgccag
     3541 cacgcctccc tgcccgccct gctgcgccac caggcccgtg agcgcggcga cgacaccgcc
     3601 gtgacagcgg cggagggccg tctcaccttc gcggaactcc actccgcggc cacgcggatg
     3661 ggctcccacc tggtcggcgc cggagtgtcg cccgacacct gcgtcgggct cttcgtggag
     3721 ccctccgccg acctggtgac aggtgtgtgg ggcatcctca ccgcaggcgc ggcctatctt
     3781 ccgctctcgc ccgactaccc ggactcccgg cttcgccaca tggcggcgga cgccggagtc
     3841 ggcgtggtcg tcacccagag ccacctgcgc gccaggctgc acggcctggt tcccgcgggc
     3901 accctggtcg tcaccgtgga cgaggccacg gacgccgacg gcgcgcaccc ccttcccgag
     3961 ccccgggagg accacctcgc ctacgtcatc cacacctccg gcagcacggg cagccccaag
     4021 ggcgtgatga tcgaacaccg cagtgtcgtc gcccagttac ggtggctgac gcgccgtggg
     4081 tatctcggcc cggcggtctc cgtcctgcag aagacgccga tcagcttcga cgcggcgcag
     4141 tgggagatcc tggcctgtgc ggcgggggcg cgggtggtca tgagtccgcc ggggtccttc
     4201 cgcgacccgc aggcggtcat cgacgcgatc cacacgtacg gcgtcacctg cctgcagggg
     4261 gtgcccaccc tgctgcgggc cctcgtggac accggtgagc tggacacctg ctcgacactg
     4321 acccggatct tctccggcgg cgaagccctc acccgggccc tcgcgcagga gctgctccgc
     4381 accctgccag gcgtctcgct ggtcaacctc tacggcccga ccgagacgac catcaacgcg
     4441 acgtccctcg aggtctcccc ggacaccctc gacgacgagg acctccacat cctgcccatc
     4501 ggcagacccg cggacggcgt cacctgccac atcctggacg agaacctccg gcccgtcggc
     4561 accgggcgga gcggggaact gttcatcggc ggggtccagg tggcccgcgg gtacgccggc
     4621 cggcccgagg cgacccggga gcgcttcctc accgcgccgt ccgcccctgg cgagcggctg
     4681 taccgcacgg gcgacctcgc ccggtggcgg cccgacggca cgatccagta cgagggccgc
     4741 accgacaacc aggtcaaact ccgcggctac cgggtcgagc tggaggaggt cgcgtcgcgc
     4801 gtcgaggagc acatgtgggt gcgcaaggcc gccgcggtgg tcacggacaa cccccgcacg
     4861 ggctcgcagg tcctggtcgc cgccgtcgaa ctcaacgaac acacggcggc ggtgatggac
     4921 caggggcgct ccgacgccgg gcaccaccag tccaagtcct ccaagctcca ggtgaaggcc
     4981 cagctcgcca accccgggct gcgggaaccg gaagaactgg caggccggac cgtcgtcccg
     5041 ctggccggcg ccgaggcgac gccggagcag cgaagagccg ccttcgcccg caagtcgtac
     5101 cggttctacg aggggggacg ggcgaccgcc gagcagctca ccgcgctgct caccccgccc
     5161 ccgccgcccg ctgccgccgc ccgccggccg ggcggactca ccccgcagga gctcggctcc
     5221 ctcctgcggt ggctcggtcc gcaccgcagc cctgaccgac tgctgccgaa gtacgcctac
     5281 gcgtcccccg gagccctgta cgccacccag ctgtacctgg agatcgacgg tgtggccggg
     5341 ctgtcgggcg tctactacca ccaccccttc gaccacaccc tcgtacgcgt cggagaccac
     5401 ccggcgccgg gccgcaccgc cgagctgaag atccacttcc tggggaagcg gcgggccgtc
     5461 gagcccgtct acaagaacaa catcgtcgag gtgctggagt tcgaggccgg gcacatgctc
     5521 ggggtcctgg aggaggtgct gccgggcatg ggcctcatgg tggccccgca cggcttcgac
     5581 ccggacgccg cggcccggct cgacgtcgcg gcggacgacc actacctggg caccttcgcg
     5641 gtggttccgc acgacggccg gccccgcccc gaggaggtgg agctgttcct ccaggtgcac
     5701 ggtgaccggg tcgagggact gcccggcggc ctctaccggc accaggacgg cacgctggtc
     5761 cgtctcgggg acgaggtggt ggagcagcgc cacgtcatcg cgatcaacca gggcgtgtac
     5821 gagcgggcct cgttcggcat cggcgccgtc gcgcggacga gcgacgactg gcgccggtac
     5881 atggtgctcg ggaccctgct gcaccgcctc cagcgcgtcg ggggcatggg gctgatgtcg
     5941 gcggggtact cctcgcggac cggccacccg ctgcccgccg cgcagcgcct ggacgacctc
     6001 ctgagccgcg cctcggtgcg cgccgccccc gcctcgtact tcttcgtggg ggggccggtc
     6061 tccgcggaac aggccgcgca cgagggcatg aacgaggacg ccgtgcacac caagggaccg
     6121 gcggagatga tccgcgacga cgccgcccag ttcctccccg actacatggt cccggcgcgg
     6181 gtcgtcgtgg tggaccagct gcccgtcacc cccaacggca agaccgatct caaagccacc
     6241 gccctcctgc ccgaggtggc cgccggccgg aacaccaccc cgcacgtcgc accggcgacg
     6301 cccaccgaac ggtggctggc cggcgagtgg ggccgcctgc tcggctacga agacgtctcc
     6361 accagggacg agttcttcgc ctccggaggc aactccctgc actccgttgc cctggtgaac
     6421 cggatcaaca gacggttcgg gacacagttc ccgctccaga cggtgttcga ggcacccacc
     6481 ctcgccgaac tggccgcccg cgtggcggag cgcgccccgg cacccgcctc caggctgata
     6541 cgcctcaaca gcgacggcgc cccggcccgg gccttcgtct ggcccggcct gggcggctac
     6601 cctatgaacc tgcgcccgct cgccctgggc ttcgacggtg agcgctcggt gaccgccgta
     6661 caggcccagg gcatcaacac cggcgagacc ccccacccca ccgtccggcg gatggcggag
     6721 gcggacatcg ccgaaatacg gcgcgtgcag ccctcggggc cgtacaccct ctggggctac
     6781 tccttcggcg cgcgggtggc cttcgaagcc gcctggcagc tggagcaggc gggcgagaag
     6841 gtggaggacc tgctgctgat ctgcccgggc aacccgcgcg tccggaccga ggacggccgg
     6901 cgccggggcc gcgaggcatc gttcgccaac ccggtctacg tgaccattct ccactccgtg
     6961 ttcaccggca ccacccacgg gcccgccgtg gccgcctgcc tgggcgagac caccgacgag
     7021 gactccttcg tcgccttcgt ccatcgcacc atgcccgcgc tgtccccgga gacgatccgg
     7081 cgcatcaccc aggtcgtcgc ggcgacctac gcgttcgaat acaccttcag cgaactgcgg
     7141 gagcgtcggg tcgaggcacc ggtgaccgtc ttcaaggcgc acggggacga ctactcgttc
     7201 ctggaggaca gttccggcta ctcggccagg ccaccgcggg tggtgcccct caccggcgac
     7261 cactacgagg tgctgaggga gcggggcgtg gccgaactga tctccggcat acgcgccttg
     7321 gacctcttcg cctaggaatt caagcttgga tccgatatca gatctcatat gggtacccca
     7381 tggtctagaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7441 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7501 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7561 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7921 tgcgctctcc tgttccgacc ctgccgctta ccggatacc
//
