LOCUS       sequence-382378-       10911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of mouse Gata2 fused to mCherry in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: pHAGE-Gata2-mCh
FEATURES             Location/Qualifiers
     source          1..10911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..30
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     80..99
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             291..924
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    971..1096
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1593..1826
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2011..2055
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2348..2465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2513..3691
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2744..3682
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3639..3659
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5147..5845
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFETIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(5277..5295)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5545..5564
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5739..5762
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5858..6425
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5875..6427
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6019..6036)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6246..6265
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6445..7044
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6445..6464)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6941..6961
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7219..7245
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7489..7518
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    7595..8183
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7648..7668)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8066..8077)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    8319..8352
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8379..8559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8606..8624)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8692..8796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8797..8865,8866..9657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9015..9034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9828..10416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10570..10587
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10827..10848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10863..10868,10869..10886,10887..10893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(10901..10911,1..6)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10906..10911,1..17)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt
       61 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
      121 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
      181 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
      241 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
      301 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
      361 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
      421 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
      481 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
      541 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
      601 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
      661 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
      721 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      781 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      841 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      901 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      961 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     1021 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     1081 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1141 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1201 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1261 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1321 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1381 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     1441 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1501 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1561 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1621 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1681 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1741 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1801 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1861 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1921 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1981 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     2041 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     2101 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     2161 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     2221 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     2281 tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca
     2341 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2401 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2461 attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt gaggctccgg
     2521 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     2581 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     2641 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     2701 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     2761 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     2821 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     2881 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     2941 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     3001 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     3061 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     3121 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     3181 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     3241 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     3301 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     3361 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     3421 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     3481 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     3541 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     3601 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     3661 aagttttttt cttccatttc aggtgtcgtg aagcggccgc atggaggtgg cgcctgagca
     3721 gcctcgctgg atggcgcacc ccgccgtatt gaatgcgcag caccccgact cgcaccatcc
     3781 gggcctggcg cataactaca tggagccagc acagctgctg cctcccgacg aggtggatgt
     3841 cttcttcaac catctcgact cgcagggcaa cccttactac gccaacccgg cccacgcgcg
     3901 cgcgcgcgtt tcctacagcc cggcgcatgc ccgtctcacc ggaggccaga tgtgccgacc
     3961 acacttgttg cacagcccag gcttgccgtg gctggacggg ggcaaagcag ctctctctgc
     4021 cgccgctgcc catcaccaca gtccctggac cgtcagcccg ttctccaaga ccccgctgca
     4081 cccctcagct gctggagcac ccggagggcc tctgtctgtt tacccagggg ctgcgggtgg
     4141 gagcggggga ggcagtggga gctccgtggc ctccctcacc cccactgcag cccactcggg
     4201 ctcccatctc ttcggcttcc cacccacgcc acccaaagaa gtgtctccag accccagcac
     4261 aacaggagct gcttccccgg cctcttcttc tgcagggggt agtgtagccc ggggtgagga
     4321 caaggatggc gtcaagtacc aagtgtcact ctccgagagc atgaagatgg aaggcggcag
     4381 tcccctgcgc ccgggcctag ctaccatggg cacccagcct gcaacacacc acccgatacc
     4441 cacctatccc tcctatgtgc ccgccgcagc tcatgactat ggcagcagtc tcttccatcc
     4501 aggaggcttc ctgggtggcc ccgcctccag cttcacccct aagcagagaa gcaaggctcg
     4561 ctcctgctca gaaggccggg agtgtgtcaa ctgtggtgcc acagccaccc ctctctggcg
     4621 acgagatggc acgggccact acctgtgcaa tgcctgtggg ctctaccaca agatgaatgg
     4681 acagaaccgg ccgctcatca agcccaagcg gaggctgtct gctgccagaa gagcgggcac
     4741 ctgttgtgca aattgtcaga cgacaaccac caccttatgg cgccggaacg ccaacgggga
     4801 ccctgtgtgc aacgcctgtg gcctctacta caagctgcac aatgttaaca ggccactgac
     4861 catgaagaag gaagggatcc agacccggaa tcggaagatg tccagcaaat ccaagaaaag
     4921 caagaaaggg gctgaatgtt tcgaggagct ctccaagtgc atgcaagaga agtcaccgcc
     4981 cttcagtgcg gctgccctgg ctggacacat ggcacctgtg ggacacctcc caccttttag
     5041 tcactctgga cacatcctac ccacgcccac gcctatccac ccttcctcca gtctctcttt
     5101 tggccacccc cacccgtcca gcatggtgac tgccatgggc ttcgaagtga gcaagggcga
     5161 ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     5221 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     5281 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     5341 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     5401 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     5461 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     5521 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     5581 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     5641 gaagctgaag gacggcggcc actacctggt ggagttcgag accatctaca tggccaagaa
     5701 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     5761 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     5821 gtacggcatg gacgagctgt acaagtagtc tagaggatcc ctcccccccc cctaacgtta
     5881 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5941 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     6001 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     6061 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     6121 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     6181 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     6241 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     6301 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     6361 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6421 gataatatgg ccacacatat gaccatgacc gagtacaagc ccacggtgcg cctcgccacc
     6481 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6541 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6601 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6661 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6721 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6781 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6841 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6901 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6961 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7021 atgacccgca agcccggtgc ctgaatcgat gttgtttctg tggaaaagag gcaggctcct
     7081 ggcaaaaggt cagagtctgg atcaccttct gctggaggcc acagcaaacc tcctcacagc
     7141 ccactggtcc tcaagaggtg ccacgtctcc acacatcagc acaactacgc agcgcctccc
     7201 tccactcgga aggactatcc tgctgccaag agggtcaagt tggacagtgt cagagtcctg
     7261 agacagatca gcaacaaccg aaaatgcacc agccccaggt cctcggacac cgaggagaat
     7321 gtcaagaggc gaacacacaa cgtcttggag cgccagagga ggaacgagct aaaacggagc
     7381 ttttttgccc tgcgtgacca gatcccggag ttggaaaaca atgaaaaggc ccccaaggta
     7441 gttatcctta aaaaagccac agcatacatc ctgtccgtcc aagcagagga gcaaaagctc
     7501 atttctgaag aggacttgtt gcggaaacga cgagaacagt tgaaacacaa acttgaacag
     7561 ctacggaact cttgtgcgta agtcgataga tcctaatcaa cctctggatt acaaaatttg
     7621 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7681 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7741 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7801 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7861 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7921 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7981 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     8041 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8101 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8161 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     8221 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8281 gaagacaaga tcacctgcag gacaggcgcg caggtaccat aacttcgtat aatgtatgct
     8341 atacgaagtt atggcgcgcc ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8401 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8461 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8521 ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccgggcgatt aaggaaaggg
     8581 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8641 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8701 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8761 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8821 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8881 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8941 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9001 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     9061 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9121 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9181 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9241 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9301 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9361 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9421 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9481 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9541 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9601 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9661 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9721 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9841 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9901 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9961 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10021 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10081 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10141 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10201 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10261 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10321 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10381 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10441 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10501 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10561 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10621 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
    10681 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    10741 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
    10801 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    10861 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg a
//
