LOCUS       sequence-382379-       10461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of mouse Gfi1b fused to mCherry in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: pHAGE-Gfi1b-mCh
FEATURES             Location/Qualifiers
     source          1..10461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    639..764
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1261..1494
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1679..1723
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2016..2133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2181..3359
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2412..3350
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3307..3327
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4365..5063
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(4495..4513)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4763..4782
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4957..4980
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5076..5643
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5093..5645
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5237..5254)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5464..5483
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5663..6262
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5663..5682)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6159..6179
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6437..6463
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6707..6736
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    6813..7401
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6866..6886)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7284..7295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    7537..7570
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7597..7777
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7824..7842)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7910..8014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8015..8083,8084..8875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8233..8252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9046..9634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9788..9805
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10045..10066
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10081..10086,10087..10104,10105..10111)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10119..10135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10124..10146
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10143..10159
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10143..10159
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10209..10228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10420..10461,1..592)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
       61 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
      121 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
      181 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
      241 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
      301 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
      361 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
      421 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      481 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      541 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      601 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      661 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      721 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      781 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      841 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      901 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      961 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1021 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1081 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1141 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1201 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1261 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1321 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1381 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1441 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1501 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1561 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1621 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1681 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1741 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1801 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1861 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1921 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1981 tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     2041 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2101 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2161 ccagtttgga ctagtcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2221 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2281 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2341 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2401 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     2461 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     2521 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     2581 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     2641 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     2701 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     2761 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     2821 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     2881 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     2941 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3001 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3061 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3121 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     3181 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3241 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3301 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa
     3361 gcggccgcat gccacggtcc tttctagtga agagtaagaa ggcacacact taccaccagc
     3421 cccgggcaca gggtgatgag ctggtctggc ctcctgctgt aattcctgtg gcaaaagagc
     3481 atagccagag tgccagccct cttctcagca caccgcttcc aagccagacc ttggactgga
     3541 acacaatcaa acaggagcgg gagatgttgc tgaaccagag ccttcccaag atggcctcag
     3601 ccccagaggg gcctctcgtg acaccccaac cccaggatgg ggaatcacca ctctctgagt
     3661 cacccccttt ctacaagccc agcttctcct gggatacctt ggcctcctcc tacagccaca
     3721 gctacacaca gaccccctcc accatgcagt ccgccttcct ggagcgctcc gtgaggctgt
     3781 acggcagccc cctcgtgccc agcacagagt ctcccttgga cttccgcctc cgctactctc
     3841 caggcatgga cacttaccac tgtgtcaagt gcaacaaggt gttctccacc cctcatgggc
     3901 tagaagtgca tgtccgccgc tctcacagcg gaacccggcc ctttgcctgt gatgtctgtg
     3961 gcaaaacctt tggccacgct gtgagcttgg agcagcatac tcacgtccac tcacaggagc
     4021 gaagcttcga gtgccggatg tgtggcaaag ccttcaagcg ttcatccacc ctgtccaccc
     4081 acctgctcat ccactcggac actcggccct acccctgcca gttctgtggg aagcgcttcc
     4141 accagaagtc ggacatgaag aaacacacct acatccacac aggtgagaag ccccacaagt
     4201 gccaggtgtg tgggaaagcc ttcagccaga gctccaacct catcacccac agccgcaagc
     4261 acacaggctt caagccgttc agctgtgagc tgtgcaccaa gggcttccag cgcaaggtgg
     4321 acctgcgacg tcaccgtgag agtcaacaca atctcaagtt cgaagtgagc aagggcgagg
     4381 aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
     4441 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     4501 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     4561 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     4621 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     4681 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     4741 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     4801 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     4861 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
     4921 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     4981 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     5041 acggcatgga cgagctgtac aagtagtcta gaggatccct cccccccccc taacgttact
     5101 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     5161 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     5221 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     5281 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     5341 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     5401 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     5461 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     5521 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     5581 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5641 taatatggcc acacatatga ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5701 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5761 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5821 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5881 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5941 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     6001 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     6061 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     6121 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     6181 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6241 gacccgcaag cccggtgcct gaatcgatgt tgtttctgtg gaaaagaggc aggctcctgg
     6301 caaaaggtca gagtctggat caccttctgc tggaggccac agcaaacctc ctcacagccc
     6361 actggtcctc aagaggtgcc acgtctccac acatcagcac aactacgcag cgcctccctc
     6421 cactcggaag gactatcctg ctgccaagag ggtcaagttg gacagtgtca gagtcctgag
     6481 acagatcagc aacaaccgaa aatgcaccag ccccaggtcc tcggacaccg aggagaatgt
     6541 caagaggcga acacacaacg tcttggagcg ccagaggagg aacgagctaa aacggagctt
     6601 ttttgccctg cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt
     6661 tatccttaaa aaagccacag catacatcct gtccgtccaa gcagaggagc aaaagctcat
     6721 ttctgaagag gacttgttgc ggaaacgacg agaacagttg aaacacaaac ttgaacagct
     6781 acggaactct tgtgcgtaag tcgatagatc ctaatcaacc tctggattac aaaatttgtg
     6841 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6901 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6961 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7021 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7081 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7141 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7201 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7261 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7321 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7381 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta
     7441 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7501 agacaagatc acctgcagga caggcgcgca ggtaccataa cttcgtataa tgtatgctat
     7561 acgaagttat ggcgcgccct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7621 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7681 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7741 ctcagaccct tttagtcagt gtggaaaatc tctagcaccc gggcgattaa ggaaagggct
     7801 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7921 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7981 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8041 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8101 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8161 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8221 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     8281 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8341 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8401 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8461 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8521 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8581 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8641 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8701 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8761 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8821 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8881 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9721 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9781 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9841 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
     9901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9961 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
    10021 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    10081 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    10141 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
    10201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10261 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10321 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    10381 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
    10441 aaagaagaca agatatcctt g
//
