LOCUS       sequence-382382-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of mouse cFos fused to mCherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: pHAGE-cFos-mCh
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(21..26,27..44,45..51)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    59..75
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     64..86
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     83..99
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     83..99
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     149..168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             360..993
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1040..1165
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1662..1895
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2080..2124
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2582..3760
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2813..3751
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3708..3728
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4916..5614
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(5046..5064)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5314..5333
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5508..5531
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5627..6194
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5644..6196
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5788..5805)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6015..6034
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6214..6813
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6214..6233)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6710..6730
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6988..7014
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7258..7287
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    7364..7952
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7417..7437)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7835..7846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    8088..8121
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8148..8328
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8375..8393)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8461..8565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8566..8634,8635..9426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8784..8803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9597..10185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10339..10356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    join(10596..10611,1..6)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
       61 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
      121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
      181 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
      241 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
      301 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
      361 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
      421 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      481 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      541 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      601 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      661 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      721 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      781 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      841 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      901 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      961 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     1021 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     1081 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     1141 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     1201 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     1261 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1321 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1381 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1441 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1501 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1561 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1621 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1681 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1741 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1801 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1861 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1921 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1981 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     2041 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     2101 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2161 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2221 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2281 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2341 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtcgtg
     2581 aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     2641 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     2701 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2761 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2821 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2881 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     2941 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     3001 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     3061 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     3121 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     3181 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     3241 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     3301 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     3361 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     3421 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     3481 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     3541 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     3601 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     3661 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     3721 agtggttcaa agtttttttc ttccatttca ggtgtcgtga agcggccgca tgatgttctc
     3781 gggtttcaac gccgactacg aggcgtcatc ctcccgctgc agtagcgcct ccccggccgg
     3841 ggacagcctt tcctactacc attccccagc cgactccttc tccagcatgg gctctcctgt
     3901 caacacacag gacttttgcg cagatctgtc cgtctctagt gccaacttta tccccacggt
     3961 gacagccatc tccaccagcc cagacctgca gtggctggtg cagcccactc tggtctcctc
     4021 cgtggcccca tcgcagacca gagcgcccca tccttacgga ctccccaccc agtctgctgg
     4081 ggcttacgcc agagcgggaa tggtgaagac cgtgtcagga ggcagagcgc agagcatcgg
     4141 cagaaggggc aaagtagagc agctatctcc tgaagaggaa gagaaacgga gaatccgaag
     4201 ggaacggaat aagatggctg cagccaagtg ccggaatcgg aggagggagc tgacagatac
     4261 actccaagcg gagacagatc aacttgaaga tgagaagtct gcgttgcaga ctgagattgc
     4321 caatctgctg aaagagaagg aaaaactgga gtttattttg gcagcccacc gacctgccta
     4381 caagatcccc gatgaccttg gcttcccaga ggagatgtct gtggcctccc tggatttgac
     4441 tggaggtctg cctgaggctt ccaccccaga gtctgaggag gccttcaccc tgccccttct
     4501 caacgaccct gagcccaagc catccttgga gccagtcaag agcatcagca acgtggagct
     4561 gaaggcagaa ccctttgatg acttcttgtt tccggcatca tctaggccca gtggctcaga
     4621 gacctcccgc tctgtgccag atgtggacct gtccggttcc ttctatgcag cagactggga
     4681 gcctctgcac agcaattcct tggggatggg gcccatggtc acagagctgg agcccctgtg
     4741 tactcccgtg gtcacctgta ctccgggctg cactacttac acgtcttcct ttgtcttcac
     4801 ctaccctgaa gctgactcct tcccaagctg tgccgctgcc caccgaaagg gcagcagcag
     4861 caacgagccc tcctccgact ccctgagctc acccacgctg ctggccctgt tcgaagtgag
     4921 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     4981 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5041 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     5101 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     5161 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5221 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     5281 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     5341 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     5401 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     5461 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     5521 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     5581 cctgttcctg tacggcatgg acgagctgta caagtagtct agaggatccc tccccccccc
     5641 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5701 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5761 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5821 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5881 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5941 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     6001 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     6061 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     6121 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     6181 aaacacgatg ataatatggc cacacatatg accatgaccg agtacaagcc cacggtgcgc
     6241 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6301 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6361 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6421 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6481 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6541 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6601 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6661 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6721 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6781 acctggtgca tgacccgcaa gcccggtgcc tgaatcgatg ttgtttctgt ggaaaagagg
     6841 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct
     6901 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca
     6961 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc
     7021 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc
     7081 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta
     7141 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc
     7201 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag
     7261 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa
     7321 cttgaacagc tacggaactc ttgtgcgtaa gtcgatagat cctaatcaac ctctggatta
     7381 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7441 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7501 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7561 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7621 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7681 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7741 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7801 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7861 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7921 gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     7981 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8041 actcccaacg aagacaagat cacctgcagg acaggcgcgc aggtaccata acttcgtata
     8101 atgtatgcta tacgaagtta tggcgcgccc tgctttttgc ttgtactggg tctctctggt
     8161 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8221 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8281 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta
     8341 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     8401 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8461 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8521 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     8821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9481 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9721 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10261 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    10321 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    10381 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
    10441 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    10501 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt
    10561 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct c
//
