LOCUS       sequence-382386-        5200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHERD30T-crRNA(Lse)_V3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Guan J, Oromi-Bosch A, Mendoza SD, Karambelkar S, Berry JD, 
            Bondy-Denomy J
  TITLE     Bacteriophage genome engineering with CRISPR-Cas13a.
  JOURNAL   Nat Microbiol. 2022 Oct 31. pii: 10.1038/s41564-022-01243-4. doi: 
            10.1038/s41564-022-01243-4.
  PUBMED    36316452
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2022 Oct 31. pii: 10.1038/s41564-022-01243-4. doi: 
            10.1038/s41564-022-01243-4."
COMMENT     Sequence Label: pSDM177
FEATURES             Location/Qualifiers
     source          1..5200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     561..580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            730..838
                     /note="incP origin of transfer"
     CDS             complement(995..1873)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1900..2184
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2107..2126
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    2245..2301
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2305..2321)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2314..2336)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2482..3315)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3329..3680
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        join(3721..3726,3727..3743,3744..3749)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3938..4471
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRHGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacaag
      721 ctagcttatc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      781 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      841 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      901 tcgtgctaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      961 tgcagcggaa agtatacctt aaggaatccc caaattatga caacttgacg gctacatcat
     1021 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
     1081 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
     1141 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
     1201 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
     1261 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     1321 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     1381 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     1441 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     1501 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     1561 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     1621 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     1681 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     1741 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     1801 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     1861 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
     1921 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
     1981 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
     2041 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
     2101 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
     2161 cgcaactctc tactgtttct ccatggtaag agactacctc tatatgaaag aggactaaaa
     2221 ccgagaccac gtacgtacgg tctcgaattc gagctcggta cccggggatc ctctagagtc
     2281 gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2341 cactggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2401 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga aaggcaggcc
     2461 gggccgtggt ggccacggcc tctaggccag atccagcggc atctgggtta gtcgagcgcg
     2521 ggccgcttcc catgtctcac cagggcgagc ctgtttcgcg atctcagcat ctgaaatctt
     2581 cccggccttg cgcttcgctg gggccttacc caccgccttg gcgggcttct tcggtccaaa
     2641 actgaacaac agatgtgtga ccttgcgccc ggtctttcgc tgcgcccact ccacctgtag
     2701 cgggctgtgc tcgttgatct gcgtcacggc tggatcaagc actcgcaact tgaagtcctt
     2761 gatcgaggga taccggcctt ccagttgaaa ccactttcgc agctggtcaa tttctatttc
     2821 gcgctggccg atgctgtccc attgcatgag cagctcgtaa agcctgatcg cgtgggtgct
     2881 gtccatcttg gccacgtcag ccaaggcgta tttggtgaac tgtttggtga gttccgtcag
     2941 gtacggcagc atgtctttgg tgaacctgag ttctacacgg ccctcaccct cccggtagat
     3001 gattgtttgc acccagccgg taatcatcac actcggtctt ttccccttgc cattgggctc
     3061 ttgggttaac cggacttccc gccgtttcag gcgcagggcc gcttctttga gctggttgta
     3121 ggaagattcg atagggacac ccgccatcgt cgctatgtcc tccgccgtca ctgaatacat
     3181 cacttcatcg gtgacaggct cgctcctctt cacctggcta atacaggcca gaacgatccg
     3241 ctgttcctga acactgaggc gatacgcggc ctcgaccagg gcattgcttt tgtaaaccat
     3301 tgggggtgag gccacgttcg acattccttg tgtataaggg gacactgtat ctgcgtccca
     3361 caatacaaca aatccgtccc tttacaacaa caaatccgtc ccttcttaac aacaaatccg
     3421 tcccttaatg gcaacaaatc cgtccctttt taaactctac aggccacgga ttacgtggcc
     3481 tgtagacgtc ctaaaaggtt taaaagggaa aaggaagaaa agggtggaaa cgcaaaaaac
     3541 gcaccactac gtggccccgt tggggccgca tttgtgcccc tgaaggggcg ggggaggcgt
     3601 ctgggcaatc cccgttttac cagtccccta tcgccgcctg agagggcgca ggaagcgagt
     3661 aatcagggta tcgaggcgga ttcacccttg gcgtccaacc agcggcacca gcggcgcgaa
     3721 ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac
     3781 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     3841 tgttatgact gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     3901 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt
     3961 tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg
     4021 cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg
     4081 tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg
     4141 gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt
     4201 tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat
     4261 ctatgatctc gcagtctccg gcgagcaccg gaggcatggc attgccaccg cgctcatcaa
     4321 tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta
     4381 cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca
     4441 ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc
     4501 ggccgcggag ttgttcggta aattgtcaca acgccgcggc caattcgact caccagtcac
     4561 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4621 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4681 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4741 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4801 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4861 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4921 gtttattgct gataaatctg gagccggtga gcgtgggtca cgcggtatca ttgcagcact
     4981 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5041 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5101 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5161 taaaaggatc taggtgaaga tcctttttga taatctcatg
//
