LOCUS       sequence-382391-       11110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YFP control expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Christen M, Kulasekara HD, Christen B, Kulasekara BR, Hoffman LR, 
            Miller SI
  TITLE     Asymmetrical distribution of the second messenger c-di-GMP upon 
            bacterial cell division.
  JOURNAL   Science. 2010 Jun 4;328(5983):1295-7. doi: 10.1126/science.1188658.
  PUBMED    20522779
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2010-06-4- 4"; volume: "328"; issue: 
            "5983"; pages: "1295-7"
COMMENT     Sequence Label: pMMB67EH-YFP
FEATURES             Location/Qualifiers
     source          1..11110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(43..48,49..64,65..71)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     84..106
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             join(139..141,142..144,145..858)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(445..464)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(903..920)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1073..1159
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1251..1278
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1297..1388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(1970..2503)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2692..2697,2698..2714,2715..2720))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(3186..3205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4265..4659
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            5001..5088
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6327..7298
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             7809..8648
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8635..9486
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(9763..10845)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10807..10826
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10846..10923)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg
       61 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaattcact
      121 ttaagaagga gatatcatat ggtgagcaaa ggcgaagagc tgttcaccgg cgtggtgccc
      181 atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag cggcgagggc
      241 gagggcgacg ccacctacgg caagctgacc ctgaagctgc tgtgcaccac cggcaagctg
      301 cccgtgccct ggcccaccct ggtgaccacc ctgggctacg gcgtgcagtg cttcgcccgc
      361 taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga gggctacgtg
      421 caggagcgga ccatcttctt caaggacgac ggcaactaca agacccgggc cgaggtgaag
      481 ttcgagggcg acaccctggt gaaccggatc gagctgaagg gcatcgactt caaggaggac
      541 ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt gtacatcacc
      601 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccggcacaa catcgaggac
      661 ggcggcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      721 ctgctgcccg acaaccacta cctgagctac cagagcaagc tgttcaagga ccccaacgag
      781 aagcgggacc acatggtgct gctggagttc ctgaccgccg ccggcatcac cgagggcatg
      841 aacgagctct ataagtgact cgagaagctt ggctgttttg gcggatgaga gaagattttc
      901 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
      961 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     1021 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     1081 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1141 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     1201 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     1261 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     1321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1561 ttcgccccga agaacgtttt ccaatgatga gcacttttca tgatgtctaa cggccaaggt
     1621 aagcggcccg cagaatgcgg gtccgcttga ccgcagagtt agacccgaag gccaaaaggg
     1681 agcgcccggc cttgaagaag ggcgcagccc aagaaaaacc gctggaccac gaaagcggcg
     1741 cagcgctgca agaaggatag ctggtggcgc ttgctgcaca agcgctggag gcccaaaagg
     1801 ctagaaagcc tttggaacag ggcggagcgc tgaaataagt ggctgcgcgc ataaagcggg
     1861 tgatgggact aacgcctgag ttaagccgga gcgctttgcg gccgcggcgt tgtgacaatt
     1921 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     1981 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     2041 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     2101 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     2161 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     2221 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     2281 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     2341 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     2401 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     2461 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     2521 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     2581 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     2641 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     2701 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     2761 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     2821 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     2881 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     2941 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     3001 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa
     3061 ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc
     3121 tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcagaaa
     3181 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     3241 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3301 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3361 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3421 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3481 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3541 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3601 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3661 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3721 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3781 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3841 gtttactcat atatacttta gattgatttc tgaaagcgac caggtgctcg gcgtggcaag
     3901 actcgcagcg aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa
     3961 attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg
     4021 ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt
     4081 aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg
     4141 tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca
     4201 gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cgtactaact
     4261 gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg aacccctgca
     4321 ataactgtca cgcccccaaa cctgcaaacc cagcaggggc gggggctggc ggggtgttgg
     4381 aaaaatccat ccatgattat ctaagaataa tccactaggc gcggttatca gcgcccttgt
     4441 ggggcgctgc tgcccttgcc caatatgccc ggccagaggc cggatagctg gtctattcgc
     4501 tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg
     4561 ctaaacccca caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc
     4621 ctttccccct acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg
     4681 atcattcagc ccggctcatc cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg
     4741 tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat cctccggcca ctcgctgctg
     4801 ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc gccttgtttc gttcttgcgc
     4861 tcttgctgct gttcccttgc ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt
     4921 tcttcgagct tggccagccg atccgccgcc ttgttgctcc ccttaaccat cttgacaccc
     4981 cattgttaat gtgctgtctc gtaggctatc atggaggcac agcggcggca atcccgaccc
     5041 tactttgtag gggagggcgc acttaccggt ttctcttcga gaaactggcc taacggccac
     5101 ccttcgggcg gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa aagcaacagc
     5161 gaggcagcat ggcgatttat caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat
     5221 cggccagggc caaggccgac tacatccagc gcgaaggcaa gtatgcccgc gacatggatg
     5281 aagtcttgca cgccgaatcc gggcacatgc cggagttcgt cgagcggccc gccgactact
     5341 gggatgctgc cgacctgtat gaacgcgcca atgggcggct gttcaaggag gtcgaatttg
     5401 ccctgccggt cgagctgacc ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc
     5461 acctgaccgg tgccgagcgc ctgccgtata cgctggccat ccatgccggt ggcggcgaga
     5521 acccgcactg ccacctgatg atctccgagc ggatcaatga cggcatcgag cggcccgccg
     5581 ctcagtggtt caagcggtac aacggcaaga ccccggagaa gggcggggca cagaagaccg
     5641 aagcgctcaa gcccaaggca tggcttgagc agacccgcga ggcatgggcc gaccatgcca
     5701 accgggcatt agagcgggct ggccacgacg cccgcattga ccacagaaca cttgaggcgc
     5761 agggcatcga gcgcctgccc ggtgttcacc tggggccgaa cgtggtggag atggaaggcc
     5821 ggggcatccg caccgaccgg gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga
     5881 tcatcgactt acaggaatac cgggaggcaa tagaccatga acgcaatcga cagagtgaag
     5941 aaatccagag gcatcaacga gttagcggag cagatcgaac cgctggccca gagcatggcg
     6001 acactggccg acgaagcccg gcaggtcatg agccagaccc agcaggccag cgaggcgcag
     6061 gcggcggagt ggctgaaagc ccagcgccag acaggggcgg catgggtgga gctggccaaa
     6121 gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg
     6181 cgggggtggc actggaagct atggctaacc gtgatgctgg cttccatgat gcctacggtg
     6241 gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc cactgacaac cgaggacggc
     6301 tcgatctggc tgcgcttggt ggcccgatga agaacgacag gactttgcag gccataggcc
     6361 gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg
     6421 gccagatgat gaaccgggaa tggtcagccg ccgaagtgct ccagaacacg ccatggctca
     6481 agcggatgaa tgcccagggc aatgacgtgt atatcaggcc cgccgagcag gagcggcatg
     6541 gtctggtgct ggtggacgac ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc
     6601 gggagcctgc cctggtagtg gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg
     6661 ccgacgccgc aggcggtgaa cttcgggggc agattgcccg gacgctggcc agcgagtacg
     6721 acgccgaccc ggccagcgcc gacagccgcc actatggccg cttggcgggc ttcaccaacc
     6781 gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca
     6841 agggcaagac cgccaccgct ggcccggcgc tggtgcagca ggctggccag cagatcgagc
     6901 aggcccagcg gcagcaggag aaggcccgca ggctggccag cctcgaactg cccgagcggc
     6961 agcttagccg ccaccggcgc acggcgctgg acgagtaccg cagcgagatg gccgggctgg
     7021 tcaagcgctt cggtgatgac ctcagcaagt gcgactttat cgccgcgcag aagctggcca
     7081 gccggggccg cagtgccgag gaaatcggca aggccatggc cgaggccagc ccagcgctgg
     7141 cagagcgcaa gcccggccac gaagcggatt acatcgagcg caccgtcagc aaggtcatgg
     7201 gtctgcccag cgtccagctt gcgcgggccg agctggcacg ggcaccggca ccccgccagc
     7261 gaggcatgga caggggcggg ccagatttca gcatgtagtg cttgcgttgg tactcacgcc
     7321 tgttatacta tgagtactca cgcacagaag ggggttttat ggaatacgaa aaaagcgctt
     7381 cagggtcggt ctacctgatc aaaagtgaca agggctattg gttgcccggt ggctttggtt
     7441 atacgtcaaa caaggccgag gctggccgct tttcagtcgc tgatatggcc agccttaacc
     7501 ttgacggctg caccttgtcc ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc
     7561 tcggtgactg atatgaaaga ccaaaaggac aagcagaccg gcgacctgct ggccagccct
     7621 gacgctgtac gccaagcgcg atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc
     7681 aagttctggc tgaccgacga cgaatacgag gcgctgcgcg agtgcctgga agaactcaga
     7741 gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac caccaactgc ctgcaaagga
     7801 ggcaatcaat ggctacccat aagcctatca atattctgga ggcgttcgca gcagcgccgc
     7861 caccgctgga ctacgttttg cccaacatgg tggccggtac ggtcggggcg ctggtgtcgc
     7921 ccggtggtgc cggtaaatcc atgctggccc tgcaactggc cgcacagatt gcaggcgggc
     7981 cggatctgct ggaggtgggc gaactgccca ccggcccggt gatctacctg cccgccgaag
     8041 acccgcccac cgccattcat caccgcctgc acgcccttgg ggcgcacctc agcgccgagg
     8101 aacggcaagc cgtggctgac ggcctgctga tccagccgct gatcggcagc ctgcccaaca
     8161 tcatggcccc ggagtggttc gacggcctca agcgcgccgc cgagggccgc cgcctgatgg
     8221 tgctggacac gctgcgccgg ttccacatcg aggaagaaaa cgccagcggc cccatggccc
     8281 aggtcatcgg tcgcatggag gccatcgccg ccgataccgg gtgctctatc gtgttcctgc
     8341 accatgccag caagggcgcg gccatgatgg gcgcaggcga ccagcagcag gccagccggg
     8401 gcagctcggt actggtcgat aacatccgct ggcagtccta cctgtcgagc atgaccagcg
     8461 ccgaggccga ggaatggggt gtggacgacg accagcgccg gttcttcgtc cgcttcggtg
     8521 tgagcaaggc caactatggc gcaccgttcg ctgatcggtg gttcaggcgg catgacggcg
     8581 gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag caagggggtg ccccgtggtg
     8641 aagcctaaga acaagcacag cctcagccac gtccggcacg acccggcgca ctgtctggcc
     8701 cccggcctgt tccgtgccct caagcggggc gagcgcaagc gcagcaagct ggacgtgacg
     8761 tatgactacg gcgacggcaa gcggatcgag ttcagcggcc cggagccgct gggcgctgat
     8821 gatctgcgca tcctgcaagg gctggtggcc atggctgggc ctaatggcct agtgcttggc
     8881 ccggaaccca agaccgaagg cggacggcag ctccggctgt tcctggaacc caagtgggag
     8941 gccgtcaccg ctgatgccat ggtggtcaaa ggtagctatc gggcgctggc aaaggaaatc
     9001 ggggcagagg tcgatagtgg tggggcgctc aagcacatac aggactgcat cgagcgcctt
     9061 tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc aggggtttcg gctgctgtcg
     9121 gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg ccctgaaccc cttgatcgcg
     9181 caggccgtca tgggtggcgg ccagcatgtg cgcatcagca tggacgaggt gcgggcgctg
     9241 gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg gctggatcga ccccggcaaa
     9301 accggcaagg cttccataga taccttgtgc ggctatgtct ggccgtcaga ggccagtggt
     9361 tcgaccatgc gcaagcgccg ccagcgggtg cgcgaggcgt tgccggagct ggtcgcgctg
     9421 ggctggacgg taaccgagtt cgcggcgggc aagtacgaca tcacccggcc caaggcggca
     9481 ggctgacccc ccccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt
     9541 attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata
     9601 ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt tcctggcttt
     9661 gcttccagat gtatgctctt ctgctcccga acgccagcaa gacgtagccc agcgcgtcgg
     9721 ccagcttgca attcgcgcta acttacatta attgcgttgc gctcactgcc cgctttccag
     9781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9841 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     9901 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     9961 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
    10021 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
    10081 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
    10141 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
    10201 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
    10261 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
    10321 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
    10381 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
    10441 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
    10501 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
    10561 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
    10621 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
    10681 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
    10741 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
    10801 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
    10861 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcacc attcgatggt
    10921 gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca agctgatccg ggcttatcga
    10981 ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt
    11041 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
    11101 gttttttgcg
//
