LOCUS       sequence-382399-       11246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It expresses HA-fused mouse Set2 isoform protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Molla MR, Shimizu A, Komeno M, Rahman NIA, Soh JEC, Nguyen LKC, Khan
            MR, Tesega WW, Chen S, Pang X, Tanaka-Okamoto M, Takashima N, Sato 
            A, Suzuki T, Ogita H
  TITLE     Vascular smooth muscle RhoA counteracts abdominal aortic aneurysm 
            formation by modulating MAP4K4 activity.
  JOURNAL   Commun Biol. 2022 Oct 7;5(1):1071. doi: 10.1038/s42003-022-04042-z.
  PUBMED    36207400
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-10-7"; volume: "5(1):1071. doi"; pages: " 
            10.1038/s42003-022-04042-z"
COMMENT     Sequence Label: pCMV-HA Set2
FEATURES             Location/Qualifiers
     source          1..11246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          32..259
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      278..287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             284..994
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(429..447)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    1017..1241
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1249..1837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1329..1348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2008..2799,2800..2868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEMGAS
                     LIKHW"
     primer_bind     2631..2650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2869..2973)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3012..3260
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3012..3032
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3183..3202
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3279..3354
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3441..4619
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3672..4610
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4567..4587
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4636..4654
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4704..4724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5319..5342,5343..5345,5346..9419)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5319..9419
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9462..9482
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10458..10478
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10490..10624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10527..10546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10581..10600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        10638..10923
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        11076..11127
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11078..11098
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        11172..11190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
       61 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
      121 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga gcaagaggta
      181 agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg agcacctgcc
      241 tgaaatcact ttttttcagg ttggcagatc cgctagcgcc accatggtga gcaagggcga
      301 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      361 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      421 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      481 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      541 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgatga
      601 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      661 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaaccat
      721 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      781 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      841 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      901 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      961 ctccaccggc ggcatggacg agctgtacaa gtaaaccggt ctcttggccc ggatccctgt
     1021 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1081 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     1141 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     1201 aaacaatagc aggcatgctg gggatgcggt gggctctatg gtggaagctt tccataggct
     1261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1681 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacccatctc
     2041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggc ttccacgctc
     2161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3001 cgtctaagta ggagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     3061 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     3121 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     3181 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3241 tgtggaaagg acgaaacacc gggtcttcga gaagacctgt tttagagcta gaaatagcaa
     3301 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     3361 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca
     3421 attctgcaga caaatggctc ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     3481 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     3541 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     3601 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     3661 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     3721 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     3781 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     3841 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3901 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     3961 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     4021 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     4081 cggcgaggcg gggcctgcga gcgcggcaac cgagaatcgg acgggggtag tctcaagctg
     4141 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     4201 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     4261 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     4321 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     4381 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     4441 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     4501 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     4561 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     4621 gaattagctt ggtactaata cgactcacta tagggagagc cgccaccatg aaacggacag
     4681 ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtctctgag gtggagtttt
     4741 cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgc gatgagaggg
     4801 aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag ggctggaaca
     4861 gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg agacagggcg
     4921 gcctggtcat gcagaactac agactgattg acgccaccct gtacgtgaca ttcgagcctt
     4981 gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg tttggcgtga
     5041 ggaactcaaa aagaggcgcc gcaggctccc tgatgaacgt gctgaactac cccggcatga
     5101 atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc ctgctgtgcg
     5161 atttctatcg gatgcctaga caggtgttca atgctcagaa gaaggcccag agctccatca
     5221 actccggagg atctagcgga ggctcctctg gctctgagac acctggcaca agcgagagcg
     5281 caacacctga aagcagcggg ggcagcagcg gggggtcaga caagaagtac agcatcggcc
     5341 tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca
     5401 gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg
     5461 gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca
     5521 gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg
     5581 agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag
     5641 aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc
     5701 acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg
     5761 ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc
     5821 tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg
     5881 tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca
     5941 aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc
     6001 tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga
     6061 cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg
     6121 acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc
     6181 tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga
     6241 acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc
     6301 accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag
     6361 agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc
     6421 aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac
     6481 tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca
     6541 gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt
     6601 tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc
     6661 cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga
     6721 gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc
     6781 agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc
     6841 ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat
     6901 acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg
     6961 tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact
     7021 tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg
     7081 cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca
     7141 atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca
     7201 gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga
     7261 agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg
     7321 gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg
     7381 ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc
     7441 agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg
     7501 gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga
     7561 aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga
     7621 ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca
     7681 aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg
     7741 agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg
     7801 acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg
     7861 acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca
     7921 acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg
     7981 ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga
     8041 gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa
     8101 agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc
     8161 tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg
     8221 atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc
     8281 tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg
     8341 tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa
     8401 tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg
     8461 agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa
     8521 ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca
     8581 tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt
     8641 ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta
     8701 agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag
     8761 tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca
     8821 tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag
     8881 aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg
     8941 gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc
     9001 cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc
     9061 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     9121 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     9181 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     9241 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     9301 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     9361 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggtgact
     9421 ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag aagaggaaag
     9481 tcctgggagg tgacagcggc gggagcggcg ggagcggggg gagcgtcacc cccgctttgc
     9541 agatgaagaa accaaagcag ttttgccgac ggatggggca aaagaagcag cgaccagcta
     9601 gagcagggca gccacacagc tcgtccgacg cagcccaggc acctgcagag cagccacaca
     9661 gctcgtccga tgcagcccag gcaccttgcc ccagggagcg ctgcttggga ccgcccacca
     9721 ctccgggccc ataccgcagc atctatttct caagcccaaa gggccacctt acccgactgg
     9781 ggttggagtt cttcgaccag ccggcagtcc ccctggcccg ggcatttctg ggacaggtcc
     9841 tagtccggcg acttcctaat ggcacagaac tccgaggccg catcgtggag accgaggcat
     9901 acctggggcc agaggatgaa gccgcccact caaggggtgg ccggcagacc ccccgcaacc
     9961 gaggcatgtt catgaagccg gggaccctgt acgtgtacat catttacggc atgtacttct
    10021 gcatgaacat ctccagccag ggggacgggg cttgcgtctt gctgcgagca ctggagcccc
    10081 tggaaggtct ggagaccatg cgtcagcttc gcagcaccct ccggaaaggc accgccagcc
    10141 gtgtcctcaa ggaccgcgag ctctgcagtg gcccctccaa gctgtgccag gccctggcca
    10201 tcaacaagag ctttgaccag agggacctgg cacaggatga agctgtatgg ctggagcgtg
    10261 gtcccctgga gcccagtgag ccggctgtag tggcagcagc ccgggtgggc gtcggccatg
    10321 caggggagtg ggcccggaaa cccctccgct tctatgtccg gggcagcccc tgggtcagtg
    10381 tggtcgacag agtggctgag caggacacac aggcctctgg cggctcaaaa agaaccgccg
    10441 acggcagcga attcgagccc aagaagaaga ggaaagtcta aacgcgtcga acttgtttat
    10501 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10561 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10621 gatcggcggc cggtacccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    10681 aacgaccccc gcccattgac gtcaatagta acgccaatag ggactttcca ttgacgtcaa
    10741 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    10801 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcattg tgcccagtac
    10861 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    10921 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
    10981 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggrrngg
    11041 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
    11101 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
    11161 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagyr smkccmkgkm
    11221 srmagmsmsm skmmtkcnat ggrwrc
//
