LOCUS       sequence-382424-       10436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Gata2 without the 
            C-terminal zinc finger (amino acids 343-379), in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: pFUW-tetO-mCh-Gata2-del-C-ZF(343-379)
FEATURES             Location/Qualifiers
     source          1..10436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        370..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             586..766
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    813..938
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1431..1664
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1849..1893
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2191..2308
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2365..2383,2384..2406,2407..2425,2426..2448,2449..2467,
                     2468..2490,2491..2509,2510..2532,2533..2551,2552..2574,
                     2575..2593,2594..2616,2617..2635)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2668..2706
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2703..2727
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2818..3519
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2951..2969)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3219..3238
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3413..3436
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4879..5467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4932..4952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5350..5361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5470..5486)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5471..5487)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5992..6172
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6198..6215)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6204..6428
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6474..6902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6561..6580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6771..6792
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6911..6931)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6916..7245
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7096..7231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7158..7177
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7293..7340
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7359..7733
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7863..7984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7900..7919)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7954..7973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8033..8049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8033..8049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8046..8068)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8057..8073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8081..8087,8088..8105,8106..8111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8126..8147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8264..8281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8435..9023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8515..8534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9194..9985,9986..10054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9817..9836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10055..10159)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10234..10253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10425..10436,1..368)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
      601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      781 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      841 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      901 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      961 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1021 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1081 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1141 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1201 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1261 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     1321 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1381 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1441 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1501 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1561 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1621 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1681 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1741 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1801 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1861 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1921 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1981 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2041 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2101 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     2161 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2221 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2281 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     2341 ttggttagat ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2401 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2461 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2521 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2581 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2641 gaaagtcgag ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc
     2701 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2761 catagaagac accgggaccg atccagcctc cgcggccccg aattcaccgg ttctagaatg
     2821 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     2881 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2941 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     3001 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     3061 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     3121 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     3181 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     3241 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     3301 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     3361 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     3421 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     3481 caccacctgt tcctgtacgg catggacgag ctgtacaagg aggtggcgcc tgagcagcct
     3541 cgctggatgg cgcaccccgc cgtattgaat gcgcagcacc ccgactcgca ccatccgggc
     3601 ctggcgcata actacatgga gccagcacag ctgctgcctc ccgacgaggt ggatgtcttc
     3661 ttcaaccatc tcgactcgca gggcaaccct tactacgcca acccggccca cgcgcgcgcg
     3721 cgcgtttcct acagcccggc gcatgcccgt ctcaccggag gccagatgtg ccgaccacac
     3781 ttgttgcaca gcccaggctt gccgtggctg gacgggggca aagcagctct ctctgccgcc
     3841 gctgcccatc accacagtcc ctggaccgtc agcccgttct ccaagacccc gctgcacccc
     3901 tcagctgctg gagcacccgg agggcctctg tctgtttacc caggggctgc gggtgggagc
     3961 gggggaggca gtgggagctc cgtggcctcc ctcaccccca ctgcagccca ctcgggctcc
     4021 catctcttcg gcttcccacc cacgccaccc aaagaagtgt ctccagaccc cagcacaaca
     4081 ggagctgctt ccccggcctc ttcttctgca gggggtagtg tagcccgggg tgaggacaag
     4141 gatggcgtca agtaccaagt gtcactctcc gagagcatga agatggaagg cggcagtccc
     4201 ctgcgcccgg gcctagctac catgggcacc cagcctgcaa cacaccaccc gatacccacc
     4261 tatccctcct atgtgcccgc cgcagctcat gactatggca gcagtctctt ccatccagga
     4321 ggcttcctgg gtggccccgc ctccagcttc acccctaagc agagaagcaa ggctcgctcc
     4381 tgctcagaag gccgggagtg tgtcaactgt ggtgccacag ccacccctct ctggcgacga
     4441 gatggcacgg gccactacct gtgcaatgcc tgtgggctct accacaagat gaatggacag
     4501 aaccggccgc tcatcaagcc caagcggagg ctgtctgctg cccacaatgt taacaggcca
     4561 ctgaccatga agaaggaagg gatccagacc cggaatcgga agatgtccag caaatccaag
     4621 aaaagcaaga aaggggctga atgtttcgag gagctctcca agtgcatgca agagaagtca
     4681 ccgcccttca gtgcggctgc cctggctgga cacatggcac ctgtgggaca cctcccacct
     4741 tttagtcact ctggacacat cctacccacg cccacgccta tccacccttc ctccagtctc
     4801 tcttttggcc acccccaccc gtccagcatg gtgactgcca tgggctaggc tagcttcgaa
     4861 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4921 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4981 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5041 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5101 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5161 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5221 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5281 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5341 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5401 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5461 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     5521 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     5581 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5641 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5701 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     5761 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     5821 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     5881 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     5941 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     6001 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6061 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6121 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     6181 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6241 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6301 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6361 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6421 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     6481 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6541 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6661 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6781 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6841 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6901 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6961 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7021 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7081 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7141 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     7201 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     7261 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     7321 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     7381 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     7441 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     7501 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     7561 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     7621 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     7681 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     7741 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7801 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7861 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7921 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7981 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8041 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8101 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8161 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8221 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8281 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    10201 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10261 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    10321 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    10381 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattat
//
