LOCUS       sequence-382438-        4125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC2A2_STOP
FEATURES             Location/Qualifiers
     source          1..4125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1682..1781)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1798..1817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1799..1817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1822..1838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1822..1838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1940..1959)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1951..2760
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2018..2037)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2907..3495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3396..3415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3649..3666
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3825..3852)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3944..4030)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4079..4101
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4093..4110
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4094..4110
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac cgaggacaag
      121 gtgaccggca cactggtgtt caccgtgatc acagccgtgc tgggctcttt ccagtttggc
      181 tacgatatcg gcgtgatcaa tgccccccag caggtcatca tcagccacta taggcacgtg
      241 ctgggcgtgc ctctggacga tcgcaaggcc atcaacaatt acgtgatcaa ctccaccgac
      301 gagctgccca caatcagcta ttccatgaat cccaagccta ccccatgggc agaggaggag
      361 acagtggcag cagcacagct gatcaccatg ctgtggtctc tgagcgtgag ctccttcgcc
      421 gtgggaggaa tgacagccag cttctttgga ggatggctgg gcgataccct gggaaggatc
      481 aaggccatgc tggtggccaa tatcctgtcc ctggtgggcg ccctgctgat gggcttttcc
      541 aagctgggcc cttctcacat cctgatcatc gccggcaggt ccatctctgg cctgtactgc
      601 ggcctgatct ccggcctggt gcctatgtat atcggcgaga tcgcaccaac cgccctgagg
      661 ggcgccctgg gcacattcca ccagctggcc atcgtgaccg gcatcctgat ctctcagatc
      721 atcggcctgg agtttatcct gggcaactac gacctgtggc acatcctgct gggactgtct
      781 ggcgtgcggg caatcctgca gagcctgctg ctgttctttt gtccagagag ccccagatac
      841 ctgtatatca agctggacga ggaggtgaag gccaagcagt ccctgaagag gctgcgcggc
      901 tacgacgatg tgacaaagga tatcaacgag atgcggaagg agagagagga ggcctctagc
      961 gagcagaagg tgtccatcat ccagctgttc accaattcct cttatcggca gcctatcctg
     1021 gtggccctga tgctgcacgt ggcccagcag ttttccggca tcaacggcat cttctactat
     1081 agcacctcca tctttcagac agccggcatc tctaagccag tgtacgccac aatcggcgtg
     1141 ggcgccgtga atatggtgtt caccgccgtg agcgtgtttc tggtggagaa ggccggccgg
     1201 agaagcctgt tcctgatcgg catgtccggc atgttcgtgt gcgccatctt tatgtctgtg
     1261 ggcctggtgc tgctgaacaa gttttcctgg atgtcttatg tgagcatgat cgccatcttc
     1321 ctgtttgtga gcttctttga gatcggcccc ggccctatcc catggttcat ggtggcagag
     1381 ttcttttccc agggaccaag gcctgccgcc ctggcaatcg ccgccttttc taactggaca
     1441 tgcaatttca tcgtggccct gtgctttcag tacatcgccg atttctgtgg cccatacgtg
     1501 ttcttcctgt tcgccggcgt gctgctggcc ttcaccctgt ttacattctt taaggtgccc
     1561 gagaccaagg gcaagagctt cgaggagatc gccgccgagt ttcagaagaa gtctggaagc
     1621 gcccacagac caaaggcagc agtggagatg aagtttctgg gcgccaccga gacagtgtaa
     1681 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1741 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1801 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1861 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1921 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1981 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2041 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2101 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2161 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2221 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2281 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2341 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2401 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2461 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2521 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2581 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2641 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2701 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2761 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2821 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3601 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3661 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3721 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3781 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3841 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3901 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3961 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4021 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4081 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
