LOCUS       sequence-38244-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of green excitation ratiometric genetically 
            encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-GEX-GECO1
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..510)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAELKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDLPEFQTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     626..645
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcctac ttcgctgtca tcatttgtac aaactcttcg tagtttacct gaccatcccc
      121 atcgatgtct gctaccctga tcatttcatc tacctcttca tctgttaact tctctccaag
      181 gtttgtcatc acgtggcgaa gctctgctgc gccgatgtag ccattgccgt ccttatcaaa
      241 cacacggaac gcttctctaa tttcctcttc actgtctgtg tcattcattt ttcttgccat
      301 cattgtctgg aactcaggga ggtcgattgt gccgtcaccg tcggcatcta cttcattgat
      361 catgtcctgc agctctgctt ctgtggggtt ctgccccaga gaccgcatca ccgtccccag
      421 ctccttggtt gttatcgtcc catccccgtc cttgtcaaat agggagaaag cctctttaag
      481 ttctgcgatc tgctcttcag tcagttggtc acgcgtgttg tactccagct tgtgccccag
      541 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      601 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      661 gcgctcctgg atgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      721 gtcggggtag cggctgaagc actgcacgcc ataggacagg gtggtcacga gggtgggcca
      781 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgta
//
