LOCUS       sequence-382440-        5097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO5A1_STOP
FEATURES             Location/Qualifiers
     source          1..5097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2654..2753)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2770..2789)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2771..2789)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2794..2810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2794..2810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2912..2931)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2923..3732
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2990..3009)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3879..4467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4368..4387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4621..4638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4797..4824)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4916..5002)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5051..5073
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5065..5082
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5066..5082
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga tgagggcacc
      121 ggcctgcagc caggagcagg agagcagctg gaggcaccag caaccgccga ggcagtgcag
      181 gagcggtgcg agcctgagac actgagatct aagagcctgc cagtgctgag ctccgccagc
      241 tgcaggccat ccctgtctcc tacctccggc gatgccaatc ctgccttcgg ctgcgtggac
      301 tctagcggcc accaggagct gaagcaggga cctaacccac tggcaccaag cccttccgcc
      361 ccatctacca gcgccggact gggcgattgt aatcacaggg tggacctgtc taagacattc
      421 agcgtgtcct ctgccctggc catgctgcag gagcggagat gcctgtacgt ggtgctgacc
      481 gattcccgct gttttctggt gtgcatgtgc ttcctgacct tcatccaggc cctgatggtg
      541 agcggctacc tgagctccgt gatcaccaca atcgagaggc gctattccct gaagtctagc
      601 gagagcggcc tgctggtgtc ctgcttcgac atcggcaacc tggtggtggt ggtgtttgtg
      661 agctacttcg gaggaagagg ccggagacca ctgtggctgg cagtgggagg actgctgatc
      721 gcctttggcg ccgccctgtt cgcactgcca cactttatct ccccccctta tcagatccag
      781 gagctgaacg cctctgcccc aaatgatgga ctgtgccagg gaggaaattc taccgccaca
      841 ctggagccac cagcatgtcc aaaggacagc ggaggaaaca atcactgggt gtatgtggcc
      901 ctgttcatct gcgcccagat cctgatcggc atgggctcca cccctatcta cacactgggc
      961 ccaacctatc tggacgataa cgtgaagaag gagaattcct ctctgtacct ggccatcatg
     1021 tatgtgatgg gcgccctggg accagcagtg ggatacctgc tgggcggcct gctgatcggc
     1081 ttttatgtgg atcctaggaa cccagtgcac ctggatcaga atgaccctcg cttcatcggc
     1141 aactggtgga gcggctttct gctgtgcgcc atcgccatgt ttctggtcat cttcccaatg
     1201 ttcacctttc ccaagaagct gcctccaagg cacaagaaga agaagaagaa gaagttcagc
     1261 gtggatgccg tgagcgacga tgacgtgctg aaggagaaga gcaacaattc cgagcaggcc
     1321 gacaagaagg tgagctccat gggctttggc aaggatgtgc gcgacctgcc tagggcagcc
     1381 gtgagaatcc tgtctaacat gaccttcctg ttcgtgagcc tgtcttacac agccgagagc
     1441 gccatcgtga cagcctttat caccttcatc ccaaagttca tcgagtccca gtttggcatc
     1501 cccgcctcta atgccagcat ctacaccggc gtgatcatcg tgccaagcgc cggagtggga
     1561 atcgtgctgg gcggctatat catcaagaag ctgaagctgg gagcaaggga gtccgccaag
     1621 ctggccatga tctgctccgg cgtgtctctg ctgtgctttt ctaccctgtt catcgtgggc
     1681 tgcgagagca tcaacctggg cggcatcaat atcccttaca ccacaggccc aagcctgaca
     1741 atgccccacc gcaacctgac cggctcctgc aacgtgaatt gcggctgtaa gatccacgag
     1801 tacgagcccg tgtgcggatc cgacggaatc acctatttca atccatgcct ggcaggatgc
     1861 gtgaactccg gaaatctgtc tacaggcatc cggaactata ccgagtgcac atgcgtgcag
     1921 tccagacagg tcatcacccc ccctacagtg ggccagaggt ctcagctgcg cgtggtcatc
     1981 gtgaagacct acctgaacga gaatggctat gccgtgagcg gcaagtgcaa gcggacctgt
     2041 aacacactga tccccttcct ggtgttcctg tttatcgtga cctttatcac agcatgcgca
     2101 cagccaagcg ccatcatcgt gacactgagg tccgtggagg atgaggagcg ccctttcgcc
     2161 ctgggcatgc agtttgtgct gctgcggaca ctggcctaca tcccaacccc catctatttc
     2221 ggcgccgtga tcgacaccac atgtatgctg tggcagcagg agtgcggagt gcagggatct
     2281 tgttgggagt acaacgtgac cagcttcaga ttcgtgtact tcggactggc agcaggcctg
     2341 aagtttgtgg gcttcatctt tatcttcctg gcctggtaca gcatcaagta taaggaggat
     2401 ggactgcaga ggcgccggca gcgggagttc cccctgagca ccgtgtccga gagagtggga
     2461 cacccagaca atgccagaac aaggtcctgc cctgccttct ctacccaggg cgagtttcac
     2521 gaggagacag gcctgcagaa gggcatccag tgtgccgccc agacataccc tggcccattt
     2581 ccagaggcaa tctctagctc cgccgaccca ggcctggagg agtcccctgc cgccctggag
     2641 ccaccctctt aagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     2701 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     2761 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2821 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     2881 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     2941 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     3001 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     3061 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     3121 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     3181 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     3241 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     3301 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     3361 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     3421 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     3481 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     3541 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     3601 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     3661 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     3721 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     3781 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     3841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4621 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4681 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4741 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     4801 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     4861 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     4921 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     4981 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     5041 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
