LOCUS       sequence-382442-        1925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1925)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046784
FEATURES             Location/Qualifiers
     source          1..1925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             272..292
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             293..319
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      440..1028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     929..948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1134..1793
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1200..1219)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1864..1898
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag actcttctat gtttcatctc gttgactttc aggttactat
      121 agcagagata ttactaatta ttatgaggac ttttaaagtt tccatttgga atcttgatta
      181 catcataaac ctcataatta aaaatttatc taagtcacta actgagaata aatattctca
      241 attagatgaa gagcaaccaa tggagattga tgagaacctg tactttcaaa gctacccgta
      301 tgacgttcct gattatgcct aaccggctta tcggtcagtt tcacctgatt tacgtaaaaa
      361 cccgcttcgg cgggtttttg cttttggagg ggcagaaaga tgaatgactg tccacgacgc
      421 tatacccaaa agaaaaagct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      841 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1021 tatggaaatt gatcgggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     1081 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
     1141 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     1201 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     1261 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     1321 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg
     1381 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     1441 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     1501 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     1561 ttttcgtttc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     1621 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1681 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1741 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgata
     1801 tcgagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     1861 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtcatcgatc tcgatcccgc
     1921 gaaat
//
