LOCUS       sequence-382445-        3551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 helicase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046911
FEATURES             Location/Qualifiers
     source          1..3551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1895..1915
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1916..1945
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      2066..2654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2555..2574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2760..3419
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2826..2845)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3490..3524
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag actcttctat ggctgtgggt gcatgtgtct tatgcaactc
      121 acagaccagc cttcgctgcg gcgcgtgcat tcgtcgtccc ttcctgtgtt gcaaatgctg
      181 ttatgaccat gtaatcagta cgtcgcacaa gctggttctg tccgttaatc cgtacgtttg
      241 caacgctccg ggatgtgatg taacagacgt gactcaactc tacctgggtg gcatgtctta
      301 ctattgcaaa agccacaaac caccgatatc ctttcctctg tgcgccaacg gccaggtatt
      361 cggtctgtac aaaaatacct gtgttgggtc tgataacgtg actgacttta acgcgatcgc
      421 aacttgcgat tggaccaacg ctggcgacta tatcctggcg aatacctgca ctgagcgatt
      481 gaaactgttc gctgctgaaa ctctgaaggc gaccgaagaa accttcaaac tctcctacgg
      541 tattgcaacg gtccgtgagg ttctatctga tcgtgaactg catctgagct gggaagtagg
      601 taagccgcgc ccgcctctga accgcaacta cgtttttact ggctatcgtg ttacaaaaaa
      661 ctctaaagtg cagatcggtg aatacacctt cgagaaaggg gactacggcg atgcagttgt
      721 ctatcggggc accactacat ataagttaaa cgtcggtgac tacttcgttc tgacttcaca
      781 cacggtgatg ccactgtccg ctccgactct ggttccgcag gaacactacg tgcgcattac
      841 tgggctgtac ccgacattga acatctcgga tgagttctcc tctaacgtag ccaattacca
      901 gaaagtaggt atgcaaaaat acagcactct gcaaggcccg cctggtaccg gaaaaagcca
      961 tttcgctata ggtctggccc tgtattaccc atccgcgcgc atcgtctaca ctgcatgcag
     1021 tcacgctgcg gtggacgcac tgtgcgaaaa ggcccttaaa tatctcccta ttgataaatg
     1081 ttctcgtatc atcccggcga gagctcgtgt tgaatgcttt gacaaattta aagttaacag
     1141 caccctggag cagtacgtgt tctgtaccgt taatgcactg ccggaaacta ccgctgatat
     1201 tgtggtgttc gacgaaatct ctatggcgac caactacgat ctgagcgtag ttaacgcgcg
     1261 tttacgtgct aaacactacg tatacatcgg cgacccagca cagctgccgg caccgaggac
     1321 tctgcttact aaaggcaccc tggaaccgga gtacttcaac tcagtttgcc gtctgatgaa
     1381 gacgatcggt ccagatatgt ttcttggcac gtgtcgcaga tgcccggcag aaattgtgga
     1441 taccgtatct gctctggttt atgataacaa actgaaagcc cataaggaca agtccgctca
     1501 gtgcttcaaa atgttttata aaggtgttat cacgcacgac gtcagtagcg cgatcaaccg
     1561 tccacagatt ggagttgtgc gagaatttct cacccgcaat ccggcatggc gtaaagctgt
     1621 cttcatctct ccctataact ctcagaacgc agtagcctcc aaaatcctgg gtctgccgac
     1681 gcagactgtg gattctagcc agggctcaga atatgattac gtgattttca cccaaaccac
     1741 tgaaaccgct cactcctgca acgttaaccg gttcaacgta gctattaccc gcgccaaagt
     1801 tggtatcctc tgcatcatgt ctgaccgcga cctgtacgac aaactgcaat ttacgagcct
     1861 cgaaatcccc cgccgtaacg tagctacttt acaggagaac ctgtactttc aaagcgagca
     1921 gaagcttatt tccgaagaag atctctaacc ggcttatcgg tcagtttcac ctgatttacg
     1981 taaaaacccg cttcggcggg tttttgcttt tggaggggca gaaagatgaa tgactgtcca
     2041 cgacgctata cccaaaagaa aaagcttgag atcctttttt tctgcgcgta atctgctgct
     2101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2641 ggagcctatg gaaattgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     2701 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     2761 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2821 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2881 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2941 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
     3001 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     3061 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     3121 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     3181 atatgttttt cgtttcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     3241 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     3301 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     3361 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     3421 ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat
     3481 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtca tcgatctcga
     3541 tcccgcgaaa t
//
