LOCUS       sequence-382447-        9674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element
            has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Bai J, Dai Y, Farinha A, Tang AY, Syal S, Vargas-Cuebas G, van 
            Opijnen T, Isberg RR, Geisinger E
  TITLE     Essential Gene Analysis in Acinetobacter baumannii by High-Density 
            Transposon Mutagenesis and CRISPR Interference.
  JOURNAL   J Bacteriol. 2021 May 20;203(12):e0056520. doi: 10.1128/JB.00565-20.
            Epub 2021 Mar 29.
  PUBMED    33782056
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2021-05-20"; volume: "203(12):e0056520. doi"; 
            pages: " 10.1128/JB.00565-20. Epub 2021 Mar 29"
COMMENT     Sequence Label: pYDE009
FEATURES             Location/Qualifiers
     source          1..9674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     584..600
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1135..1668)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1857..1862,1863..1879,1880..1885))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(2019..2645)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     2550..2569
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        2661..2716
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    2697..2715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2734..2745
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2752..2778,2779..2781,2782..5268,5269..5271,
                     5272..6858)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      6882..6953
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6969..6996
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     mobile_element  7191..7356
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            7515..7623
                     /note="incP origin of transfer"
     rep_origin      complement(7855..8443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7935..7954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8614..9405,9406..9474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9237..9256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9475..9579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9647..9665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgacgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcag gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacttggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgagct cactagtctt aagacccact ttcacattta
     2041 agttgttttt ctaatccgca tatgatcaat tcaaggccga ataagaaggc tggctctgca
     2101 ccttggtgat caaataattc gatagcttgt cgtaataatg gcggcatact atcagtagta
     2161 ggtgtttccc tttcttcttt agcgacttga tgctcttgat cttccaatac gcaacctaaa
     2221 gtaaaatgcc ccacagcgct gagtgcatat aatgcattct ctagtgaaaa accttgttgg
     2281 cataaaaagg ctaattgatt ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct
     2341 aaatgtactt ttgctccatc gcgatgactt agtaaagcac atctaaaact tttagcgtta
     2401 ttacgtaaaa aatcttgcca gctttcccct tctaaagggc aaaagtgagt atggtgccta
     2461 tctaacatct caatggctaa ggcgtcgagc aaagcccgct tattttttac atgccaatac
     2521 aatgtaggct gctctacacc tagcttctgg gcgagtttac gggttgttaa accttcgatt
     2581 ccgacctcat taagcagctc taatgcgctg ttaatcactt tacttttatc taatctagac
     2641 atcattaatt cctaattttt gttgacactc tatcgttgat agagttattt taccactccc
     2701 tatcagtgat agagaaaaga attcaaaaga tctaaagagg agaaaggatc tatggataag
     2761 aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat
     2821 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc
     2881 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt
     2941 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag
     3001 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag
     3061 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta
     3121 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta
     3181 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag
     3241 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa
     3301 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca
     3361 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa
     3421 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct
     3481 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa
     3541 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga
     3601 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca
     3661 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa
     3721 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt
     3781 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt
     3841 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg
     3901 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg
     3961 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg
     4021 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc
     4081 ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca
     4141 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat
     4201 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca
     4261 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa
     4321 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa
     4381 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa
     4441 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt
     4501 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat
     4561 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg
     4621 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt
     4681 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct
     4741 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg
     4801 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca
     4861 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat
     4921 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt
     4981 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg
     5041 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga
     5101 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat
     5161 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat
     5221 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca
     5281 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat
     5341 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg
     5401 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct
     5461 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa
     5521 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac
     5581 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt
     5641 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat
     5701 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa
     5761 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct
     5821 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg
     5881 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc
     5941 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg
     6001 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc
     6061 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa
     6121 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc
     6181 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta
     6241 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa
     6301 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt
     6361 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga
     6421 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa
     6481 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag
     6541 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca
     6601 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt
     6661 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct
     6721 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta
     6781 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt
     6841 cagctaggag gtgactaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc
     6901 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     6961 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctaggga tatattccgc
     7021 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     7081 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtctgcag
     7141 gaattcctcg agaagcttgg gcccggtacc tcgcgaaggc cttgcaggcc aaccagataa
     7201 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta
     7261 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc
     7321 acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat
     7381 gtttgggcgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7441 cgctcttccg cttcctcgct cactgacccg ctgcgctcgg tcgttcggct gcggcgagcg
     7501 gtatcagagc ttatcggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
     7561 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
     7621 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
     7681 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
     7741 ggttatgcag cggaaagtat accttaagga atcccctgat aacgcaggaa agaacatgtg
     7801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcgagtcaga ggtggcgaaa
     7921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9601 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     9661 gaggcccttt cgtc
//
