LOCUS       sequence-38245-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of green excitation ratiometric genetically 
            encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-GEX-GECO1
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..80
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     293..314
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(681..700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggatcaccat ggtcgactca tcacgtcgta agtggaataa gacaggtcac gcagtcagag
       61 ctataggtcg gctgagctca ctcgagaacg tctatatcaa ggccgacgag cagaagaacg
      121 gcatcaaggc gaacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgcct
      181 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      241 acctgagcgt gcagtccata ctttcgaaag accccaacga gaagcgtgat cacatggtcc
      301 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg
      361 gtaccggagg gagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      421 aggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggtgagg
      481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      541 tgccctggcc caccctcgtg accaccctgt cctatggcgt gcagtgcttc agccgctacc
      601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacatccagg
      661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      781 acatcctggg gcacaagctg gagtacaaca cgcgtgacca actgactgaa gagcagatcg
      841 cagaacttaa agaggctttc tccctatttg acaaggacgg ggatgggacg ataacaacca
      901 aggagctggg gacggtgatg cggtct
//
