LOCUS       sequence-382450-       10740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Test fluorescent properties in Neurospora crassa. Nuclear envelope 
            fluorescent reporter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
  TITLE     Optimized fluorescent proteins for 4-color and photoconvertible 
            live-cell imaging in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2022 Dec 5:103763. doi: 
            10.1016/j.fgb.2022.103763.
  PUBMED    36481248
REFERENCE   2  (bases 1 to 10740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763."
COMMENT     Sequence Label: csr-1_son-1_sGFP
FEATURES             Location/Qualifiers
     source          1..10740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(924..971)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1344..1362
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1400..1422)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1522..1541
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1565..1780
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             1781..2584
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(2715..3170)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2852..2873)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3064..3083
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             6097..6108
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(6597..6599,6600..6602,6603..7316)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(6642..6663)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6903..6922)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7250..7271
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8561..8583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8572..8588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8596..8602,8603..8620,8621..8626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8641..8662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8779..8796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8950..9538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9030..9049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9709..10500,10501..10569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10332..10351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10570..10674)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(10701..10740,1..1303)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
       61 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
      121 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
      181 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
      241 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
      301 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
      361 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
      421 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
      481 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
      541 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
      601 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
      661 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
      721 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
      781 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
      841 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg
      901 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
      961 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
     1021 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
     1081 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
     1141 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
     1201 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
     1261 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatactaa gaaaccatta
     1321 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     1381 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     1441 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     1501 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatacca
     1561 ccttttcaat tcatcatttt ttttttattc ttttttttga tttcggtttc cttgaaattt
     1621 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
     1681 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
     1741 actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
     1801 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
     1861 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
     1921 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
     1981 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
     2041 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
     2101 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     2161 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
     2221 tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
     2281 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
     2341 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
     2401 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
     2461 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
     2521 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
     2581 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
     2641 ttaattatat cagttattac cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2701 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     2761 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     2821 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     2881 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2941 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3001 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3061 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     3121 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     3181 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     3241 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     3301 tcacgacgct ggagaagctc attccacccg aaatgcccgt gaacttgccc ttgtttgcgc
     3361 gggccttctt gcgctctgct cggatacgct cgacatcgct gaggagctcg gcgagttcct
     3421 tggcgcggtt gcggacgttg ataccctggt ctttgccgtt ctggtcgata aaatggaact
     3481 ggcgcagcat cttgaggagg gtaagatgcg accgagcatc gtccaccact cgttcggaac
     3541 cgtgcttaat caggtactct agtagttgaa gggccttgta gatctggcgc cattcttcgg
     3601 cggacttttc tgtgaaacgg cggtaaatca tgggcatgat ttcgttgagt gtttggctaa
     3661 gaacaaggag ggaaacgcaa gttagccgcg ttcgtataca agcgagcagt tctcgagaac
     3721 gtactagttg aaggtgccat cggcgatctc ttgcatcaaa gtcgacgatg caccccaagg
     3781 ttcattgttg gtggcctcgc gcacctgggt tcgctgtcag ctcccgttcc atggtctctt
     3841 gtctgggatc aagtatatgg cgaagggcgc aagagaatgg tgccagtgtg gcgcgtggat
     3901 acgggcatac cttggactcc atctcggtat agttcatgac agctggttgg agccattgtc
     3961 agcaccctga acacgaaatc atgagggagt cttggttctg gactaggtgg tcacctacca
     4021 ttttgaacct ttcgtacgcc agccttgatg tcgtagagtg tcaggttgga cacggtatcc
     4081 ttgaggctat tgaaatccat tgcgtaaacg tgtctatcgc cggagttgct gggaattgtt
     4141 ctgatgggct aaggtaattc ttctctagat ccaatctgag cctcgcggtg atgattgcca
     4201 gacgggcggg atgctgtggc gacttggtgg tttttgttcg ggctgatcgc tttcagggcg
     4261 acgtttcacg gtcttgtcgg gatcagatgg acattgaggt gcggaattat acgatttagg
     4321 tgactgcagg ccatacagat ctccctagac gccgggccaa tccgttcctt gcaccttctc
     4381 tcacacatgc aggttttgga atgtggcttc atcgtgtcca gaactgggcg cattggggcc
     4441 aggctcagtg tcagtgtagg gatcgatgta catatggcag ttgacagcga gagagccagg
     4501 tgtcacaggc agtccgtcag tggccgcggt ggtggccacg cactttctgc aggagccgtc
     4561 tggggtgcgt gcagctggcg gccgcttccg ggcatacggc ttgtgtaacg agggtgttga
     4621 ccctcggctt ggcgcttgaa cttggcgctg cggtcccccc acagttgacc acaatccccg
     4681 gccaatcgaa cgacggcaac aagggcgcac tagccagtga acgcctgggg atagtgggtg
     4741 gtcaaagaga attgggcaaa cgagacgaca atggagggtg aaccgggaat aataaacagc
     4801 tggtgagacc acccatcaaa cgaatcaccc caccatggga tgccctcaga tgccctccat
     4861 gccaccataa ggaatgatct tacggcttgc cgaagttaac aggcctgctt ttgcatatga
     4921 acgagagcct ggtgttgcat tccacattgg ctatctacag tacctaccca acctacacac
     4981 cctgatgcga aagggacaga aggggagaag gatacataca tatacctgac tcgtgcccat
     5041 caacaacaga gaaagggtga tcgctgaaca catcagagca tctcttcccc cctggcgact
     5101 aaaagtgctc ccaatctcgt cttacatctg atacgttgtt caaactccgc aacccgatct
     5161 gcacactgcc ctgccttggc ccttcttgga gtctccgctt ccccacgcca tctcacgctc
     5221 gaggactccg ctgctaggta tgagctaggc ctgccgtcga caggatccac cagacgtcaa
     5281 caataaccca tcttggaaat acataagaag acgtcacttc cctccctttt cgaaaggaag
     5341 agatcatcat cagcatccat catggctggt ctttttggtt ccgccgccgc atccgcgagc
     5401 aacaccctcg gcgatcttaa gcaggatgtc gagctcggcc aaccccccga ggacagcatc
     5461 agcgaccttg ccttcaaccc caaccccgcc gaccagaagg acttcctcgc cgtagccagc
     5521 tgggacaaga agacacgcat ttacgagatc ttgagtaatg gtcaaggtca gggacaggcc
     5581 atgattgagc atgatgcccc cgttttctct tgcgatttct tcaaggtatg tttgcgtcga
     5641 ttggcctggc aggtagcccg acaaaacact gacatgcact tctcccaaca ggacggcaca
     5701 aaggtcattt ccgccggcgc cgacaaggcc gccaaggttc tcgatttggc caccggtcag
     5761 tcgatgcagg tcgctgctca cgatatgccc atcaagtgcg ttcgctactt cgaggccaat
     5821 ggcacgccca tggcggtgac aggtggctgg gacaagcaga tcaagtactg ggatttccgc
     5881 tccgcgaacc cggccgccac ggtgcaggcc caggagcgcg tttataccat ggatgtgcgc
     5941 gacaacttgc tcgtggtcgg aaccgctgat cgctatatca acgtgatcaa cctgaaggac
     6001 cccggcaagt tctacaagac aatgcagagc cctcttaaat ggcagactcg tgtggtcagc
     6061 tgcttcaacg attctcaggg ctttgctatc ggctcgatcg agggtagatg cgccattcaa
     6121 tatgtggagg acaaggattc tgcgtacgcc ttcacctcct ccccctttac tatcccatct
     6181 gccgaatacg ctgactaact gacttgtttt ccagctccaa cttctctttc aaatgccacc
     6241 gcgaccccgc ccaaggcaac accacggccg tccacgctgt caacgacatc tccttccacc
     6301 cccaacacgg caccttcagc acagccggta gcgacggcac cttccacttc tgggacaagg
     6361 acgccaagca ccgtctcaag ggttacccca acgtcggcgg cagcatcacc tcgacaacgt
     6421 tcaacaagac cggcagcatc tttgcctacg ccattagtta tgactggagc aaggggtacc
     6481 aaggcaactc gcccacgtac ccgaccaagg tcatgctcca cccagtacag caggacgagt
     6541 gcaagccccg tccgagcgtt aagaagaggg ctggtattgg tttggctatt gctggcatgg
     6601 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     6661 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     6721 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     6781 tgaccacctt cacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     6841 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     6901 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     6961 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     7021 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     7081 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     7141 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     7201 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     7261 tggagttcgt gaccgccgcc gggatcactc acggcatgga cgagctgtac aagtgactag
     7321 aggatccgac actatagaat acctagacag tttgcaggtc atgtgaatgt tatcgccaac
     7381 acattgaatg ctaaacagat tacctccaac gcctagatga aaccaaatta cccttgcctt
     7441 cttttttttt acgccgttaa tgcagttgtg atagagacca tgcaacgtct tctaaacgga
     7501 gacacaaaca cattcgagtc gatcagatcg aaatgacttg ttcctgtacc tcgtcaccat
     7561 ccagccaccg tctgaaatag acgcagttgc tggggctgac aggtccgcct tcctcgcaca
     7621 tatcatagac agggcaccgc ccacaaggcg cctccataag gccacttgca ctcggctcaa
     7681 ctcccataaa tgaggtaaca atgcctcctt tggttggatc atggtcgagg cgtcgttgat
     7741 tgcacgtcct cggatcctgt ttggggatgc gcgtcacacg gtaccccttg cgcccattga
     7801 ccttgacagg ctctactaag ccatcgtagt atagcacatc caccaactgc tggatatccg
     7861 cctcgctcaa cgtcgtatta ttcgtgatgc cggcgtggtg gatgaacttt gcaatgtccg
     7921 acacagtcgg gtacgacttg taaccggccg ggagaggcag caattgcggc ttgccgggcc
     7981 tggtcttctt ggccggtgcc ggtgccgccg tgtcgtcgtt ggaaatctcg cccgcgctgc
     8041 gcttcgtccc cttggaaata tcctccgagt ggggaatggt cccctgaacc acgcccttct
     8101 tgggattctt ttcacgctgc tgtgctcctc ctccttgtgc tcctactcct ggtactccag
     8161 caccgccgcc gtgggaagta tatgtcgatt tctgcttgat aaagtcaaac acaatttcct
     8221 gtagctcgtt gatgaaggcc gtgtcgagct cgccctcagt aaaccagggg ccacctgtgg
     8281 cgcgatcgga gggacgtagg ttcgccttga tgtacatctt cttgttgggg tgctcgacgt
     8341 tcttcatgct ggcgatgtag cccttttgct cgagatactt gatgcaatgc ttcataagcg
     8401 agtcggccat gttgagcttg ttcttgatgg tccggttcca gatgccgtcg gccccggctt
     8461 cgtcgaccat ggcgtatacc atcattattt cggagttggg gagggaggtg taccttttgg
     8521 gaggaagggg aggttagttg gtgtgctcgg ttggctgttt cctgtgtgaa attgttatcc
     8581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8701 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgtgtat
     8761 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9361 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10501 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10561 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10681 tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
//
