LOCUS       sequence-382457-        6002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6002)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD His6 Sumo TEV LIC cloning vector (8S)
FEATURES             Location/Qualifiers
     source          1..6002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(21..67)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     220..237
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(298..318)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(325..618)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(637..654)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             674..696
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(730..1014)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(788..807)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1041..1919
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             3555..3746
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3740..3762)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3848..3988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3903..3922
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4003..4020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4174..4762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4254..4273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4932..5723,5724..5792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5555..5574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5793..5897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5965..5983
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
       61 ttatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
      121 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg
      181 ccaggcaaat tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggctg
      241 aaaatcttct ctcatccgcc aaaacagcca agggatccgt tatccacttc caatattgga
      301 ttggaagtac aggttttcct cgatcccacc aatctgttct ctgtgagcct caataatatc
      361 gttatcctcc atgtccaaat cttcaggggt ctgatcagct tgaattctaa taccgtcgta
      421 caagaatctt aaggagtcca tttccttacc ctgtctttta gcgaacgctt ccatcagcct
      481 tcttaaagga gtggtctttt tgatcttgaa gaagatctct gaagatccat cggacacctt
      541 taaattgatg tgagtctcag gcttgacttc tggcttgacc tctggcttag cttcttgatt
      601 gacttctgag tccgacatgc tagccataga agaaccatgg tgatggtgat ggtgagaaga
      661 tttcatatgt atatctcctt cttaaagtta aacaaaatta tttctagatg ttagcccaaa
      721 aaaacgggta tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta agatccgcta
      781 atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt
      841 ggacttttct gccgtgatta tagacacttt tgttacgcgt ttttgtcatg gctttggtcc
      901 cgctttgtta cagaatgctt ttaataagcg gggttaccgg tttggttagc gagaagagcc
      961 agtaaaagac gcagtgacgg caatgtctga tgcaatatgg acaattggtt tcttctctga
     1021 atggcgggag tatgaaaagt atggctgaag cgcaaaatga tcccctgctg ccgggatact
     1081 cgtttaatgc ccatctggtg gcgggtttaa cgccgattga ggccaacggt tatctcgatt
     1141 tttttatcga ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc
     1201 agggggtggt gaaaaatcag ggacgagaat ttgtttgccg accgggtgat attttgctgt
     1261 tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc
     1321 agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatct
     1381 ttgccaatac ggggttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt
     1441 ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa
     1501 atctgcttga gcaattgttg ctgcggcgca tggaagcgat taacgagtcg ctccatccac
     1561 cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca
     1621 attttgatat agccagcgtc gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacgtc
     1681 ttttccgcca gcagttaggg attagcgtct taagctggcg cgaggaccaa cgtatcagcc
     1741 aggcgaagct gcttttgagc accacccgga tgcctatcgc caccgtcggt cgcaatgttg
     1801 gttttgacga tcaactctat ttctcgcggg tatttaaaaa atgcaccggg gccagcccga
     1861 gcgagttccg tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcataat
     1921 tggtaacgaa tcagacaatt gacggctcga gggagtagca tagggtttgc agaatccctg
     1981 cttcgtccat ttgacgggca cattatggcg gccgcgatcc tctacgccgg acgcatcgtg
     2041 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catgaccgat
     2101 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     2161 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     2221 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     2281 aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     2341 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     2401 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     2461 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     2521 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     2581 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     2641 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     2701 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     2761 ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
     2821 tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
     2881 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
     2941 acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
     3001 aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
     3061 gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
     3121 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
     3181 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
     3241 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
     3301 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
     3361 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
     3421 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
     3481 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
     3541 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
     3601 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
     3661 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
     3721 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3781 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3841 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     3901 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     3961 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     4021 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4081 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4141 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4261 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4381 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4501 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4561 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4621 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4681 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4741 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4861 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4921 ttggtcccgg gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5041 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5221 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     5281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5521 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     5581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatg ttattgaagc
     5821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     5941 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     6001 tt
//
