LOCUS       sequence-382459-        5629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5629)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD LIC cloning vector (8A)
FEATURES             Location/Qualifiers
     source          1..5629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(21..67)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     220..237
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     RBS             301..323
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(357..641)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(415..434)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             668..1546
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(1705..1724)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             3182..3373
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3367..3389)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3475..3615
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3530..3549
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3630..3647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3801..4389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3881..3900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4559..5350,5351..5419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5182..5201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5420..5524)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5592..5610
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
       61 ttatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
      121 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg
      181 ccaggcaaat tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggctg
      241 aaaatcttct ctcatccgcc aaaacagcca agggatcctt atggagttgg gatatctata
      301 tctccttctt aaagttaaac aaaattattt ctagatgtta gcccaaaaaa acgggtatgg
      361 agaaacagta gagagttgcg ataaaaagcg tcaggtaaga tccgctaatc ttatggataa
      421 aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa tcaatgtgga cttttctgcc
      481 gtgattatag acacttttgt tacgcgtttt tgtcatggct ttggtcccgc tttgttacag
      541 aatgctttta ataagcgggg ttaccggttt ggttagcgag aagagccagt aaaagacgca
      601 gtgacggcaa tgtctgatgc aatatggaca attggtttct tctctgaatg gcgggagtat
      661 gaaaagtatg gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt ttaatgccca
      721 tctggtggcg ggtttaacgc cgattgaggc caacggttat ctcgattttt ttatcgaccg
      781 accgctggga atgaaaggtt atattctcaa tctcaccatt cgcggtcagg gggtggtgaa
      841 aaatcaggga cgagaatttg tttgccgacc gggtgatatt ttgctgttcc cgccaggaga
      901 gattcatcac tacggtcgtc atccggaggc tcgcgaatgg tatcaccagt gggtttactt
      961 tcgtccgcgc gcctactggc atgaatggct taactggccg tcaatctttg ccaatacggg
     1021 gttctttcgc ccggatgaag cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat
     1081 taacgccggg caaggggaag ggcgctattc ggagctgctg gcgataaatc tgcttgagca
     1141 attgttgctg cggcgcatgg aagcgattaa cgagtcgctc catccaccga tggataatcg
     1201 ggtacgcgag gcttgtcagt acatcagcga tcacctggca gacagcaatt ttgatatagc
     1261 cagcgtcgca cagcatgttt gcttgtcgcc gtcgcgtctg tcacgtcttt tccgccagca
     1321 gttagggatt agcgtcttaa gctggcgcga ggaccaacgt atcagccagg cgaagctgct
     1381 tttgagcacc acccggatgc ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca
     1441 actctatttc tcgcgggtat ttaaaaaatg caccggggcc agcccgagcg agttccgtgc
     1501 cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg tcataattgg taacgaatca
     1561 gacaattgac ggctcgaggg agtagcatag ggtttgcaga atccctgctt cgtccatttg
     1621 acgggcacat tatggcggcc gcgatcctct acgccggacg catcgtggcc ggcatcaccg
     1681 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     1741 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     1801 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     1861 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     1921 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     1981 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     2041 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     2101 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     2161 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     2221 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     2281 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     2341 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     2401 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     2461 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2521 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     2581 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     2641 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     2701 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     2761 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     2821 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     2881 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
     2941 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
     3001 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
     3061 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
     3121 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
     3181 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
     3241 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
     3301 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
     3361 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     3421 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     3481 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     3541 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3601 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3661 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3721 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3781 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3841 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3901 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3961 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4021 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4081 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4141 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4201 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     4261 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4321 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4381 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4441 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4501 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtcccgggtt
     4561 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4621 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4681 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4741 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4801 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4861 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4921 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4981 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5041 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5101 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5161 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5221 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5281 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5341 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5401 ggaaatgttg aatactcata ctcttccttt ttcaatgtta ttgaagcatt tatcagggtt
     5461 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5521 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5581 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatt
//
