LOCUS       sequence-382467-        5693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5693)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD Strep TEV LIC cloning vector (8R)
FEATURES             Location/Qualifiers
     source          1..5693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(21..67)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     220..237
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(298..318)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(325..348)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             365..387
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(421..705)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(479..498)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             732..1610
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(1769..1788)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             3246..3437
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3431..3453)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3539..3679
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3594..3613
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3694..3711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3865..4453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3945..3964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4623..5414,5415..5483))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5246..5265
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5484..5588)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5656..5674
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
       61 ttatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
      121 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg
      181 ccaggcaaat tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggctg
      241 aaaatcttct ctcatccgcc aaaacagcca agggatccgt tatccacttc caatattgga
      301 ttggaagtac aggttttcgg cgcctttttc gaactgcggg tggctccagc tagccatatg
      361 tatatctcct tcttaaagtt aaacaaaatt atttctagat gttagcccaa aaaaacgggt
      421 atggagaaac agtagagagt tgcgataaaa agcgtcaggt aagatccgct aatcttatgg
      481 ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc
      541 tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt
      601 acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga
      661 cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga
      721 gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg
      781 cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg
      841 accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg
      901 tgaaaaatca gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag
      961 gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt
     1021 actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaatc tttgccaata
     1081 cggggttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa
     1141 tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg
     1201 agcaattgtt gctgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata
     1261 atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata
     1321 tagccagcgt cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacgt cttttccgcc
     1381 agcagttagg gattagcgtc ttaagctggc gcgaggacca acgtatcagc caggcgaagc
     1441 tgcttttgag caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg
     1501 atcaactcta tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc
     1561 gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa ttggtaacga
     1621 atcagacaat tgacggctcg agggagtagc atagggtttg cagaatccct gcttcgtcca
     1681 tttgacgggc acattatggc ggccgcgatc ctctacgccg gacgcatcgt ggccggcatc
     1741 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     1801 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     1861 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     1921 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     1981 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2041 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2101 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     2161 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     2221 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     2281 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     2341 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     2401 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     2461 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     2521 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     2581 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     2641 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     2701 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     2761 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     2821 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     2881 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     2941 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
     3001 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
     3061 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt
     3121 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca
     3181 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg
     3241 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     3301 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     3361 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg
     3421 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     3481 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     3541 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     3601 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     3661 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3901 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3961 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4021 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4081 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4141 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4201 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4261 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     4321 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4381 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4441 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4501 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4561 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtcccg
     4621 ggttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4921 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5221 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     5281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5461 acacggaaat gttgaatact catactcttc ctttttcaat gttattgaag catttatcag
     5521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5581 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     5641 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga att
//
