LOCUS       sequence-382469-        5767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5767)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD empty polycistronic destination vector (8D)
FEATURES             Location/Qualifiers
     source          1..5767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(21..67)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     220..237
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(422..438)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(495..779)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(553..572)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             806..1684
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(1843..1862)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             3320..3511
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3505..3527)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3613..3753
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3668..3687
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3768..3785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3939..4527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4019..4038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4697..5488,5489..5557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5320..5339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5558..5662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5730..5748
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
       61 ttatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
      121 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg
      181 ccaggcaaat tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggctg
      241 aaaatcttct ctcatccgcc aaaacagcca ggccggccag tactttaatt aacgtctatc
      301 agggcgatgg caccaggtgc gcgagcggcc gcgactggga aaaccctggc gggcgcgccg
      361 atatccctgc aggatgtgaa attgttatcc gctgcgatcg ccccgggact cgagtgaatt
      421 cactggccgt cgttttacag gatcttgtac agggcccgga tccgaggcct tctagatagc
      481 ccaaaaaaac gggtatggag aaacagtaga gagttgcgat aaaaagcgtc aggtaagatc
      541 cgctaatctt atggataaaa atgctatggc atagcaaagt gtgacgccgt gcaaataatc
      601 aatgtggact tttctgccgt gattatagac acttttgtta cgcgtttttg tcatggcttt
      661 ggtcccgctt tgttacagaa tgcttttaat aagcggggtt accggtttgg ttagcgagaa
      721 gagccagtaa aagacgcagt gacggcaatg tctgatgcaa tatggacaat tggtttcttc
      781 tctgaatggc gggagtatga aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg
      841 atactcgttt aatgcccatc tggtggcggg tttaacgccg attgaggcca acggttatct
      901 cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc tcaccattcg
      961 cggtcagggg gtggtgaaaa atcagggacg agaatttgtt tgccgaccgg gtgatatttt
     1021 gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc gcgaatggta
     1081 tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta actggccgtc
     1141 aatctttgcc aatacggggt tctttcgccc ggatgaagcg caccagccgc atttcagcga
     1201 cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg agctgctggc
     1261 gataaatctg cttgagcaat tgttgctgcg gcgcatggaa gcgattaacg agtcgctcca
     1321 tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc acctggcaga
     1381 cagcaatttt gatatagcca gcgtcgcaca gcatgtttgc ttgtcgccgt cgcgtctgtc
     1441 acgtcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg accaacgtat
     1501 cagccaggcg aagctgcttt tgagcaccac ccggatgcct atcgccaccg tcggtcgcaa
     1561 tgttggtttt gacgatcaac tctatttctc gcgggtattt aaaaaatgca ccggggccag
     1621 cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc
     1681 ataattggta acgaatcaga caattgacgg ctcgagggag tagcataggg tttgcagaat
     1741 ccctgcttcg tccatttgac gggcacatta tggcggtcgc gatcctctac gccggacgca
     1801 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     1861 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     1921 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     1981 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     2041 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2101 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2161 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     2221 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     2281 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     2341 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     2401 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     2461 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     2521 ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
     2581 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
     2641 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     2701 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
     2761 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
     2821 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     2881 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     2941 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     3001 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     3061 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     3121 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
     3181 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
     3241 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
     3301 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
     3361 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
     3421 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc
     3481 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     3541 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     3601 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     3661 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     3721 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     3781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4681 taaacttggt cccgggttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4741 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4801 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4861 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4921 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4981 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     5041 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5101 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5161 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5221 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5281 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     5341 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5401 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5461 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5521 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatgttatt
     5581 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5641 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     5701 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     5761 aagaatt
//
