LOCUS       sequence-382511-        8755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for 
            auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology 
            arms for integration into the genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 8755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT AID*-6MYC osTIR1
FEATURES             Location/Qualifiers
     source          1..8755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2655..2786
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             2811..2840
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2847..2876
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2892..2921
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2928..2957
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2964..2993
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3009..3038
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3045..3074
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3580..3981
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4030..4050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4066..5787
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(5982..6002)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5982..6000)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6021..6037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6021..6037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6034..6056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6045..6061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6069..6075,6076..6093,6094..6099))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6114..6135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6252..6269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6423..7011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6503..6522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7182..7973,7974..8042))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7805..7824
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8043..8147)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8184..8687
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8728..8746
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atcccctaaa gatccagcca aacctccggc caaggcacaa gttgtgggat
     2701 ggccaccggt gagatcatac cggaagaacg tgatggtttc ctgccaaaaa tcaagcggtg
     2761 gcccggaggc ggcggcgttc gtgaaggtat cacccgggtt aattaacggt gaacaaaagc
     2821 taatctccga ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg
     2881 gactcgacgg tgaacaaaag ttgatttctg aagaagattt gaacggtgaa caaaagctaa
     2941 tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac ttgaacggac
     3001 tcgacggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa aagctaatct
     3061 ccgaggaaga cttgaacggt gaacaaaaat taatcaatca ctagtgaatt cgcgccactt
     3121 ccttaagagt tcttctttga gatatcgatt gaacgagctc gaattcatcg atctatatta
     3181 ccctgttatc cctagcgtaa ctatgccaaa tgacaataag acacccaata ggtccagcac
     3241 tcccaagttt actaaaaaac cagtaacccc gaatgataaa attcctgaaa gacgggtgta
     3301 caatatggac ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct
     3361 tcgttggtct ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag
     3421 acaagacata atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac
     3481 ataacgaact aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta
     3541 ccctttttcc atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt
     3601 tttttctttt ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaatgat
     3661 ggaagacact aaaggaaaaa attaacgaca aagacagcac caacagatgt cgttgttcca
     3721 gagctgatga ggggtatctc gaagcacacg aaactttttc cttccttcat tcacgcacac
     3781 tactctctaa tgagcaacgg tatacggcct tccttccagt tacttgaatt tgaaataaaa
     3841 aaaagtttgc tgtcttgcta tcaagtataa atagacctgc aattattaat cttttgtttc
     3901 ctcgtcattg ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct
     3961 ataccaagca tacaatcaac tatctcatat acaactagaa tggataaagc ggaattaatt
     4021 cccgagcctc caaaaaagaa gagaaaggtc gaattgggta ccgccacgta cttcccggag
     4081 gaggtggtgg agcacatctt cagcttcctg ccggcgcagc gcgaccgcaa cacggtctcg
     4141 ctcgtctgca aggtgtggta cgagatcgag aggctgagcc gccgcggcgt cttcgtgggc
     4201 aactgctacg ccgtgcgcgc cggccgcgtc gccgcgcggt tccccaacgt gcgggcgctc
     4261 acggtgaagg ggaagcccca cttcgccgac ttcaacctcg tgccccccga ctggggcggc
     4321 tacgcggggc cgtggatcga ggcggccgcg aggggatgcc acggcctgga ggagctcagg
     4381 atgaagcgga tggtggtgtc cgacgagagc ctcgagctgc tggctcgctc gttcccgcgg
     4441 ttcagggctc ttgttcttat cagctgcgag gggttcagca ctgacgggct agccgccgtc
     4501 gcgagccatt gcaagcttct gagggagttg gatttgcagg aaaatgaagt ggaggatcga
     4561 gggcctaggt ggctttcctg cttccctgat tcctgcacat cacttgtctc attgaatttt
     4621 gcctgcatca aaggggaggt taatgctggt tcactggaga gacttgttag caggtcccca
     4681 aacctgcgga gtttgaggct gaatcgatct gtatcggtag atacacttgc aaagatacta
     4741 ctgcgtaccc ctaacttgga ggatttgggg acagggaatt tgacagatga cttccaaact
     4801 gagtcctact ttaagcttac cagtgctctg gagaaatgca agatgttgag gagtttgtct
     4861 ggattctggg atgcttctcc tgtttgcctg tcatttatct accccctgtg tgctcaactg
     4921 acaggattga acttgagcta tgcacccaca cttgatgctt ctgaccttac aaaaatgatt
     4981 agccgctgtg tgaagctcca acgcctttgg gtactggatt gtatctcgga caaaggcttg
     5041 caagtggtgg cctccagttg caaagacttg caagaactca gggtatttcc atcagatttc
     5101 tacgtagctg gttattctgc agtgacagag gagggacttg ttgcagtatc cttgggctgt
     5161 ccaaaactga actcactact gtacttctgt caccaaatga ctaatgctgc actagttact
     5221 gtcgccaaga actgtccaaa tttcacacga ttcagacttt gtattcttga gccagggaag
     5281 cctgatgttg tgacaagcca accattagat gaaggctttg gagctattgt tcgtgagtgc
     5341 aagggattac aacgtttgtc aatatctggt cttctcacag acaaagtttt catgtatatt
     5401 gggaaatatg caaaacaact tgagatgctt tctatagcat ttgctggtga cagtgataag
     5461 ggtatgatgc atgttatgaa tggatgcaag aatttaagga aactggagat aagagatagc
     5521 ccgtttggtg atgctgcact cttggggaat tttgctaggt acgagacaat gcgatccctt
     5581 tggatgtcat cttgcaatgt cacgttaaag gggtgccaag tccttgcgtc aaagatgccg
     5641 atgctcaatg ttgaggtcat aaatgagcgg gatggtagca atgaaatgga ggaaaaccat
     5701 ggagatctgc ctaaagtgga gaaattatat gtgtaccgca caactgctgg ggcgagggat
     5761 gatgcaccaa attttgttaa aatcctagtc gactccggtt ctgctgctag tggttgaacg
     5821 cgtggcgcct ggaaaaagat tttcaagtga ataaggagat aaaaccgtat tgacttttta
     5881 tcattctaca aaagaccagt aacgaagtgt accctgctat attaccctgt tatccctagc
     5941 gtaactcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     6001 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6061 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6121 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6181 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6241 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6841 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6961 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7021 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7081 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7141 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7201 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7261 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7321 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7381 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7441 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7501 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7561 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7621 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7681 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7741 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7801 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7861 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7921 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7981 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8041 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8101 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8161 aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa taattataat ttaaattttt
     8221 taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt
     8281 tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc
     8341 ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac
     8401 cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt
     8461 taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt
     8521 taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt
     8581 tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt
     8641 ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt ctaagaaacc
     8701 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
