LOCUS       sequence-382518-        4885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of vioC, truncated vioD and tdMCP form a J23105 
            constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Guo H, Ryan JC, Song X, Mallet A, Zhang M, Pabst V, Decrulle AL, 
            Ejsmont P, Wintermute EH, Lindner AB
  TITLE     Spatial engineering of E. coli with addressable phase-separated 
            RNAs.
  JOURNAL   Cell. 2022 Sep 29;185(20):3823-3837.e23. doi: 
            10.1016/j.cell.2022.09.016.
  PUBMED    36179672
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09"; journalName: "Cell"; date: "2022-09-29- 
            29"; volume: "185"; issue: "20"; pages: "3823-3837.e23"
COMMENT     Sequence Label: pVioCD53
FEATURES             Location/Qualifiers
     source          1..4885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(120..708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(200..219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(879..1670,1671..1739))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1502..1521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1740..1844)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1912..1930
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1934..1977)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1980..2001
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        2036..2070
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             2075..2086
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        3494..3543
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3567..3578
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    4732..4752
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      4753..4810
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(4834..4851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
       61 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      121 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      361 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      421 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      541 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      721 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      781 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      841 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
      901 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      961 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1021 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1081 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1141 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1201 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1261 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1321 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1381 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1441 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     1501 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     1561 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatata acccactcgt
     1621 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1681 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     1741 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     1801 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1861 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1921 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
     1981 aattcgcggc cgcttctaga gcgccgaaaa ccccgcttcg gcggggtttt gccgctttac
     2041 ggctagctca gtcctaggta ctatgctagc tgccaaagag gagaaatact agatggatgc
     2101 acaaacccgc cgtcgtgaac gtcgtgcgga gaagcaagca cagtggaaag caaataccgg
     2161 tggaggcggt tcaaccagcg gaggtggtgg cagtaccaaa cgtgcgatta tcgttggtgg
     2221 cggcctggcg ggtggcctga ccgcgatcta cctggcgaag cgtggctacg aagtgcacgt
     2281 cgtggagaag cgtggtgatc ctctgcgcga tctgagctct tacgtggacg ttgttagcag
     2341 ccgtgcgatc ggcgtgagca tgaccgttcg tggtatcaag agcgttttgg ctgcgggcat
     2401 tccgcgtgca gagctggatg cgtgtggcga accgatcgtg gcaatggctt tctccgtggg
     2461 tggtcagtat cgcatgcgcg aactgaagcc gttggaggat ttccgtccgc tgagcttgaa
     2521 ccgtgcggcg tttcaaaagc tgctgaacaa atacgcgaac ctggcaggcg ttcgttacta
     2581 ctttgagcat aagtgcctgg atgttgacct ggatggtaag agcgtgttga ttcagggcaa
     2641 agatggtcag ccgcagcgtc tgcaaggtga catgattatc ggtgcggatg gcgcccacag
     2701 cgccgtccgt caggcgatgc agagcggcct gcgtcgtttc gagttccagc aaacgttctt
     2761 ccgccatggc tacaaaaccc tggttttgcc ggacgcgcaa gcactgggtt accgtaaaga
     2821 cacgctgtac tttttcggca tggattccgg tggcctgttc gcgggtcgtg cggctacgat
     2881 cccagatggt agcgtcagca tcgccgtttg cctgccgtac tcgggtagcc cttccctgac
     2941 gaccaccgac gaaccgacga tgcgtgcgtt cttcgatcgt tacttcggtg gcctgccgcg
     3001 tgacgcgcgt gacgaaatgc tgcgtcagtt tctggcgaag ccgagcaacg acctgattaa
     3061 cgtgcgctct agcacctttc actataaggg taatgtgctg ttgctgggtg atgctgcgca
     3121 tgcgactgcg ccgttcctgg gtcagggtat gaacatggcg ctggaggacg cccgcacgtt
     3181 tgtcgagctg ctggaccgcc accagggcga ccaagacaaa gcctttccgg agttcacgga
     3241 gctgcgcaaa gtccaggcag acgcaatgca agacatggct cgcgccaact atgacgtttt
     3301 gagctgctcg aacccgatct ttttcatgcg tgcgcgttac acgcgttaca tgcattccaa
     3361 gtttccgggc ctgtatccgc cggatatggc cgagaaactg tactttacga gcgagccgta
     3421 cgatcgtctg caacaaatcc agcgtaaaca gaatgtttgg tacaagattg gtcgcgtgaa
     3481 ttaataaatt cagctgtcac cggatgtgct ttccggtctg atgagtccgt gaggacgaaa
     3541 cagcctctac aaataatttt gtttaaaaag aggagaaata ctagatgaag attctggtca
     3601 ttggtgctgg tccagctggt ctggttttcg catcccaact gaagcaggca cgccctttgt
     3661 gggccattga catcgtggag aagaatgacg agcaagaagt gctgggctgg ggtgtcgtgc
     3721 tgcctggccg tccgggtcag cacccggcga acccgctgtc ctatctggat gcaccggagc
     3781 gtctgaatcc gcaatttctg gaggacttca aactggtgca tcataatgag ccgtccttga
     3841 tgtccacggg cgttttgttg tgcggcgtgg agcgtcgcgg tctggttcac gcgctgcgcg
     3901 ataagtgccg cagccaaggc attgctattc gtttcgaaag cccgttgctg gaacacggtg
     3961 agctgccgct ggcggactat gatctggtgg tcctggctaa tggtgttaat cacaaaaccg
     4021 cgcatttcac cgaggctctg gtcccgcagg tggactacgg ccgcaataag tacatttggt
     4081 atggcactag ccagctgttc gatcagatga atctggtttt tcgtacccat ggtaaagata
     4141 tctttatcgc gcatgcctat aagtatagcg ataccatgag cacgttcatt gtcgaatgta
     4201 gcgaagagac ttacgcacgc gcacgcctgg gcgaaatgtc cgaagaggcg agcgcagaat
     4261 acgttgctaa ggtgttccag gccgagctgg gtggtcacgg cctggtgagc cagccgggtc
     4321 tgggttggcg taacttcatg acgttgtctc atgaccgttg tcatgatggt aagtatctct
     4381 agcaattcac gctcacaggc ttacaaagta acttgttcgg ttcgtcagag cagtgcacag
     4441 aaccgtaagt acactattaa ggttgaagta ccgaaagttg caactcagac ggtaggtggc
     4501 gtcgaacttc ctgtagctgc ctggcgttcg tatctgaata tggaactgac gatccccatt
     4561 tttgcaacta actctgattg cgagttgatt gtgaaagcga tgcaagggct tctgaaggac
     4621 ggcaatccaa ttcccagcgc aatcgcggcc aatagtggga tttactaata aggaaacaca
     4681 gaaaaagccc gcacctgaca gtgcgggctt ttttttttcg accaaaggag ctactagtag
     4741 cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
     4801 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     4861 cgttcggctg cggcgagcgg tatca
//
