LOCUS       sequence-382555-       12347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The lentiviral vector for NIR-GECO2(I426C) and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12347)
  TITLE     Plasmids for KOBACHI mice project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCSIIbleo-6507-P2A-EGFP
FEATURES             Location/Qualifiers
     source          1..12347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2414..2531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2587..3749
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2818..3740
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3697..3717
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5281..5337
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5347..5349,5350..5352,5353..5865)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(5392..5413)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5653..5672)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6000..6021
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6080..6628
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6224..6241)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6451..6470
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6631..7005
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7041..7629
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7094..7114)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7512..7523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8148..8328
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8354..8371)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8360..8584
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8630..9058
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8717..8736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8927..8948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9067..9087)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9072..9402
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9253..9388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9315..9334
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9450..9497
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9516..9890
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10020..10141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10057..10076)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10111..10130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10190..10206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10190..10206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10203..10225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10214..10230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10238..10244,10245..10262,10263..10268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10283..10304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10421..10438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10592..11180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10672..10691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11351..12142,12143..12211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11974..11993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12212..12316)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagatct ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcta caaatggcag
     2401 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2461 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2521 ttcaaaattt tcgggtttat tacagggaca gcagaaattc actttgaatt aattcaagct
     2581 tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     2641 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     2701 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     2761 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     2821 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     2881 tgaattactt ccacctggct ccagtacgtg attcttgatc ccgagctgga gccaggggcg
     2941 ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     3001 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     3061 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     3121 ttgtaaatgc gggccaggat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     3181 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     3241 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     3301 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     3361 cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg acgcggcgct
     3421 cgggagagcg ggcgggtgag tcacccacac aaaggaaagg ggcctttccg tcctcagccg
     3481 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctgga
     3541 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     3601 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     3661 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     3721 gtttttttct tccatttcag gtgtcgtgaa cacgctaccg gtctcgacga attcgccacc
     3781 atgtcggtac cgctgactac ctcagcattc ggccacgcgt ttctggctaa ctctgaacgt
     3841 gagcagatcc acctggcggg ctccattcag ccgcacggta tcctgctggc tgttaaagag
     3901 cctgacaacg tggtgatcca ggcttctatt aacgctgcgg agttcctgaa caccaacttt
     3961 gttgttggcc gtccgctgcg tgacctgggc ggcgatctgc ctttgcagat cctgccgcac
     4021 ctgaacggcc cgctgcacct ggctccgatg accctgcgtt gtactgtggg ttctccgccg
     4081 cgtcgtgtgg actgtaccat tcatcgaccg tctaacggcg gcctgatcgt agaactggaa
     4141 ccagcaacca aggccactaa cattgcgccg gctctggtcg gtgcgcttca tcgtatcact
     4201 tcttcatcct ccctgatggg cctgtgtgac gaaaccgcga ctattttccg tgagattact
     4261 ggtttcgacc gtgtgatggt aatgcgtctc ggcgcgcttg acgatctgac tgaagagcag
     4321 atcgcagaga ttaaagaggc tttctcccta tttgacaagg acggggacgg gacgataaca
     4381 accaaggagc tggggacggt gttccggtct ctggggcaga accccacaga agcagagctg
     4441 caggacatga tcaatgaagt agatgccgat ggcgacggca tattcgactt ccctgagttc
     4501 ctgacgatga tggcaaggaa aatgaatgac acagacagtg aagaggaaat tagaggagcg
     4561 ttccgcgtgt ttgataagga cggcaatggc tacatcggcg cagcagagct tcgccacgtg
     4621 atgacagacc ttggtgagaa gttaactgat gaggaggttg atgaaatgat cagggtagca
     4681 gacaacgatg gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag
     4741 ggtggcggag gttctgtaga ttcatcacgt cgtaagtgga ataaggcagg tcacgcagtc
     4801 agagctatag gtcggctgag ctcgcgtagg catgatttgc tgtccgaatg tcgtcgtgcg
     4861 gacctggaag cgttcctagg taaccgctac ccggcgtcta ctattccgca gatcgctcgt
     4921 cgcctgtacg aactcaaccg tgttcgcctg ctggtagatg tgaactatac tccggttccg
     4981 ctagagccgc gcatcagccc gctgaacggt cgtgatctgg atatgtccct gtcttgcctg
     5041 cgatcgatgt ccccgtgcca ccagaaatac atgcaggaca tgggcgttgg cgcaaccctg
     5101 gtttgctctc tgatggtgtc tggtcgtctg tggggtctga tcgtttgcca ccactacgaa
     5161 ccgcgctacg ttccgtccca cattcgcgct gctggcgaag cgctggcgga agcatgtgcg
     5221 aaccgcatcg cgacgctgga gagctttgca cagtctcagt ccaaactcga gggaagcgga
     5281 gcgacgaatt ttagtctact gaaacaagcg ggagacgtgg aggaaaaccc tggacctggc
     5341 ggccgcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5401 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     5461 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5521 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5581 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5641 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5701 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5761 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5821 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5881 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5941 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     6001 atggtcctgc tggagttcgt gaccgccgcc tctagcaacg gtttccctct agcgggatca
     6061 attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     6121 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     6181 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     6241 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     6301 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     6361 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     6421 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     6481 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     6541 tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg
     6601 tggttttcct ttgaaaaaca cgataatacc atggccaagt tgaccagtgc cgttccggtg
     6661 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     6721 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     6781 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     6841 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     6901 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     6961 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgaactag agttaacatc
     7021 gagggatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     7081 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7141 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7201 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7261 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7321 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7381 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7441 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7501 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7561 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7621 cctccccgca tcgataccgt cgagacctgg aaaaacatgg agcaatcaca agtagcaaca
     7681 cagcagctac caatgctgct tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7741 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7801 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7861 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     7921 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7981 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     8041 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     8101 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     8161 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8221 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8281 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     8341 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     8401 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     8461 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     8521 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     8581 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     8641 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8701 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8761 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg
     8821 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8881 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8941 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9001 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     9061 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga
     9121 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     9241 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9301 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     9361 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     9421 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     9481 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     9541 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     9601 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     9661 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     9721 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     9781 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     9841 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     9901 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     9961 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
    10021 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10081 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10141 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
    10201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10261 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    10321 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10381 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10441 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
    10801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    11041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11401 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    11461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
