LOCUS       sequence-382588-        9717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VHL Expression vector with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, 
            Perrimon N, Signoretti S, Kaelin WG Jr
  TITLE     HIF-independent synthetic lethality between CDK4/6 inhibition and 
            VHL loss across species.
  JOURNAL   Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 
            10.1126/scisignal.aay0482.
  PUBMED    31575731
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2019-10-1"; volume: "12(601). pii: 12/601/eaay0482.
            doi"; pages: " 10.1126/scisignal.aay0482"
COMMENT     Sequence Label: pLX304-VHL-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             623..649
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1287..1311)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1313..1354
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(1396..1970,1971..1973,1974..1982)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1563..1580)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1790..1809
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1983..1985,1986..1988,1989..2702)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1983..1985,1986..1988,1989..2699)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2028..2049)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2289..2308)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2636..2657
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2725..3313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2778..2798)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3196..3207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3385..3618
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3696..3817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3733..3752)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3787..3806
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3857..3992
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4012..4031)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4013..4031)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4041..4058)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4041..4057)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4050..4072)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4199..4654
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4286..4305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4496..4517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4680..4784
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4785..4853,4854..5645)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5003..5022)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5816..6404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6305..6324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6558..6575
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6692..6713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6728..6733,6734..6751,6752..6758)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6766..6782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6771..6793
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6790..6806
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6790..6806
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6825..6845
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6827..6845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6873..7099
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7100..7280
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7327..7452
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7945..8178
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8363..8407
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8582..9082
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9001..9019
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9094..9489
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9549..9666
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagttggca tgtacccata cgatgttcca gattacgctc cccggagggc
      661 ggagaactgg gacgaggccg aggtaggcgc ggaggaggca ggcgtcgaag agtacggccc
      721 tgaagaagac ggcggggagg agtcgggcgc cgaggagtcc ggcccggaag agtccggccc
      781 ggaggaactg ggcgccgagg aggagatgga ggccgggcgg ccgcggcccg tgctgcgctc
      841 ggtgaactcg cgcgagccct cccaggtcat cttctgcaat cgcagtccgc gcgtcgtgct
      901 gcccgtatgg ctcaacttcg acggcgagcc gcagccctac ccaacgctgc cgcctggcac
      961 gggccgccgc atccacagct accgaggtca cctttggctc ttcagagatg cagggacaca
     1021 cgatgggctt ctggttaacc aaactgaatt atttgtgcca tctctcaatg ttgacggaca
     1081 gcctattttt gccaatatca cactgccagt gtatactctg aaagagcgat gcctccaggt
     1141 tgtccggagc ctagtcaagc ctgagaatta caggagactg gacatcgtca ggtcgctcta
     1201 cgaagatctg gaagaccacc caaatgtgca gaaagacctg gagcggctga cacaggagcg
     1261 cattgcacat caacggatgg gagattgccc aactttcttg tacaaagtgg ttggtaagcc
     1321 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcc gctacgtaaa
     1381 ttccgccccc ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     1441 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     1501 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     1561 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     1621 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     1681 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     1741 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     1801 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     1861 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     1921 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     1981 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2041 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2101 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2161 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2221 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2281 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2341 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2401 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2461 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2521 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2581 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2641 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     2701 aaaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     2761 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2821 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2881 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2941 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3001 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3061 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3121 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3181 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3241 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3301 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3361 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3421 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3481 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3541 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3601 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     3661 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     3721 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3781 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     3841 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3901 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3961 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     4021 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4081 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4141 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4561 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4621 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4681 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4741 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4801 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4861 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg cgcacgagtg ggttacatcg
     4921 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4981 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5041 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5101 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5161 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5221 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5281 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5341 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5401 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5461 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5521 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5581 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5641 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5701 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5941 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6481 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6541 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6601 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6661 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6721 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6781 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     6841 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     6901 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     6961 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     7021 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     7081 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     7141 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7201 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7261 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7321 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7381 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7441 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7501 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7561 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7621 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7681 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7741 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     7801 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     7861 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     7921 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     7981 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8041 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8101 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8161 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8221 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8281 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8341 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8401 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8461 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8521 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8581 cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     8641 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     8701 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     8761 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     8821 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     8881 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     8941 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     9001 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     9061 aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc tcaagaagaa
     9121 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     9181 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     9241 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     9301 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     9361 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     9421 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     9481 tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat ggcagtattc
     9541 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     9601 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     9661 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagggg
//
