LOCUS       sequence-382610-        9622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutant VHL Expression vector with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, 
            Perrimon N, Signoretti S, Kaelin WG Jr
  TITLE     HIF-independent synthetic lethality between CDK4/6 inhibition and 
            VHL loss across species.
  JOURNAL   Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 
            10.1126/scisignal.aay0482.
  PUBMED    31575731
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2019-10-1"; volume: "12(601). pii: 12/601/eaay0482.
            doi"; pages: " 10.1126/scisignal.aay0482"
COMMENT     Sequence Label: pLX304-VHLdeltaB-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             623..649
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1194..1218)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1220..1261
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(1301..1875,1876..1878,1879..1887)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1468..1485)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1695..1714
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1888..1890,1891..1893,1894..2607)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1888..1890,1891..1893,1894..2604)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1933..1954)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2194..2213)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2541..2562
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2630..3218
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2683..2703)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3101..3112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3290..3523
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3601..3722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3638..3657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3692..3711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3762..3897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3917..3936)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3918..3936)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3946..3963)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3946..3962)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3955..3977)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4104..4559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4191..4210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4401..4422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4585..4689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4690..4758,4759..5550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4908..4927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5721..6309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6210..6229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6463..6480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6597..6618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6633..6638,6639..6656,6657..6663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6671..6687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6676..6698
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6695..6711
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6695..6711
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6730..6750
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6732..6750
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6778..7004
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7005..7185
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7232..7357
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7850..8083
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8268..8312
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8487..8987
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8906..8924
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8999..9394
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9454..9571
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagttggca tgtacccata cgatgttcca gattacgctc cccggagggc
      661 ggagaactgg gacgaggccg aggtaggcgc ggaggaggca ggcgtcgaag agtacggccc
      721 tgaagaagac ggcggggagg agtcgggcgc cgaggagtcc ggcccggaag agtccggccc
      781 ggaggaactg ggcgccgagg aggagatgga ggccgggcgg ccgcggcccg tgctgcgctc
      841 ggtgaactcg cgcgagccct cccaggtcat cttctgcaat cgcagtccgc gcgtcgtgct
      901 gcccgtatgg ctcaacgcag ggacacacga tgggcttctg gttaaccaaa ctgaattatt
      961 tgtgccatct ctcaatgttg acggacagcc tatttttgcc aatatcacac tgccagtgta
     1021 tactctgaaa gagcgatgcc tccaggttgt ccggagccta gtcaagcctg agaattacag
     1081 gagactggac atcgtcaggt cgctctacga agatctggaa gaccacccaa atgtgcagaa
     1141 agacctggag cggctgacac aggagcgcat tgcacatcaa cggatgggag attgcccaac
     1201 tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     1261 gtagtaatga gctagccgct acgtaaattc cgcccccccc cccctctccc tccccccccc
     1321 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     1381 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     1441 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     1501 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     1561 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     1621 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     1681 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     1741 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     1801 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     1861 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg
     1921 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1981 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2041 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     2101 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2161 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2221 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2281 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2341 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2401 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2461 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2521 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2581 ctcggcatgg acgagctgta caagtaaacc ggtggcgcgt taagtcgaca atcaacctct
     2641 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2701 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2761 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2821 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2881 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2941 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3001 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     3061 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3121 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     3181 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     3241 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     3301 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     3361 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3421 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3481 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     3541 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     3601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3721 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     3781 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3841 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3901 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     3961 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4021 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4081 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4141 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4201 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4261 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4321 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4381 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4441 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4501 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     4561 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4921 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5221 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5401 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5581 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5641 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6121 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6361 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6481 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6541 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6601 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6661 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6721 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     6781 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     6841 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     6901 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     6961 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     7021 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7081 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7141 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7201 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     7261 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     7321 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     7381 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     7441 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     7501 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     7561 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     7621 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     7681 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     7741 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     7801 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7861 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7921 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     7981 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     8041 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     8101 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8161 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     8221 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     8281 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     8341 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     8401 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     8461 cccacctccc aaccccgagg ggacccttgc gccttttcca aggcagccct gggtttgcgc
     8521 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     8581 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     8641 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     8701 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     8761 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     8821 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     8881 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     8941 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gtaccaccat
     9001 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     9061 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     9121 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     9181 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     9241 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     9301 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     9361 tgaattgctg ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aagaattcta
     9421 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     9481 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9541 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     9601 actttggcgc cggctcgagg gg
//
