LOCUS       sequence-382711-       19256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sr43 is a wheat stem rust resistance gene transferred from tall 
            wheat grass (Thinopyrum ponticum) into bread wheat (Triticum 
            aestivum)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19256)
  AUTHORS   Yu G, Matny O, Gourdoupis S, Rayapuram N, Aljedaani FR, Wang YL, 
            Nurnberger T, Johnson R, Crean EE, Saur IM, Gardener C, Yue Y, 
            Kangara N, Steuernagel B, Hayta S, Smedley M, Harwood W, Patpour M, 
            Wu S, Poland J, Jones JDG, Reuber TL, Ronen M, Sharon A, Rouse MN, 
            Xu S, Holusova K, Bartos J, Molnar I, Karafiatova M, Hirt H, Blilou 
            I, Jaremko L, Dolezel J, Steffenson BJ, Wulff BBH
  TITLE     The wheat stem rust resistance gene Sr43 encodes an unusual protein 
            kinase.
  JOURNAL   Nat Genet. 2023 Jun;55(6):921-926. doi: 10.1038/s41588-023-01402-1. 
            Epub 2023 May 22.
  PUBMED    37217714
REFERENCE   2  (bases 1 to 19256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-023-01402-1"; journalName: "Nat Genet"; date: 
            "2023-06"; volume: "55"; issue: "6"; pages: "921-926"
COMMENT     Sequence Label: pGGG-Sr43
FEATURES             Location/Qualifiers
     source          1..19256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     misc_feature    640..664
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(764..1016)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          1716..1905
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             complement(2787..2804)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(2807..3649)
                     /label=Act1 promoter
                     /note="rice actin promoter"
     misc_feature    17248..17272
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(17363..17951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17443..17462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(18122..18937)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(18122..18937)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     18845..18864
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     18929..18948
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(19228..19256,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt tagtcatggt tgatgggctg cctgtatcga gtggtgattt
      601 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
      661 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
      721 ttgaacactc tgtgggtctc atgccgaatt cggatccagc gtcgatctag taacatagat
      781 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
      841 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
      901 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
      961 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     1021 tgacaagcct attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     1081 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     1141 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     1201 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     1261 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     1321 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     1381 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     1441 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     1501 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     1561 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     1621 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     1681 gccgcagcga tcgcatccat ggcctccgcg accggctgca gttatcatca tcatcataga
     1741 cacacgaaat aaagtaatca gattatcagt taaagctatg taatatttac accataacca
     1801 atcaattaaa aaatagatca gtttaaagaa agatcaaagc tcaaaaaaat aaaaagagaa
     1861 aagggtccta accaagaaaa tgaaggagaa aaactagaaa tttacctgca gaacagcggg
     1921 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     1981 ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     2041 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     2101 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     2161 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     2221 cgcggtgagt tcaggctttt tcattggctt ctacctacaa aaaagctccg cacgaggctg
     2281 catttgtcac aaatcatgaa aagaaaaact accgatgaac aatgctgagg gattcaaatt
     2341 ctacccacaa aaagaagaaa gaaagatcta gcacatctaa gcctgacgaa gcagcagaaa
     2401 tatataaaaa tataaaccat agtgcccttt tcccctcttc ctgatcttgt ttagcatggc
     2461 ggaaatttta aaccccccat catctccccc aacaacggcg gatcgcagat ctacatccga
     2521 gagccccatt ccccgcgaga tccgggccgg atccacgccg gcgagagccc cagccgcgag
     2581 atcccgcccc tcccgcgcac cgatctgggc gcgcacgaag ccgcctctcg cccacccaaa
     2641 ctaccaaggc caaagatcgt gtccgagacg gaaaaaaaaa acggagaaag aaagaggaga
     2701 ggggcggggt ggttaccggc gcggcggcgg cggaggggga ggggggagga gctcgtcgtc
     2761 cggcagcgag gggggaggag gtggaggtgg tggtggtggt ggtggtaggg ttggggggat
     2821 gggaggagag gggggggtat gtatatagtg gcgatggggg gcgtttcttt ggaagcggag
     2881 ggagggccgg cctcgtcgct ggctcgcgat cctcctcgcg tttccggccc ccacgacccg
     2941 gacccacctg ctgttttttc tttttctttt ttttctttct tttttttttt ttggctgcga
     3001 gacgtgcggt gcgtgcggac aactcacggt gatagtgggg gggtgtggag actattgtcc
     3061 agttggctgg actggggtgg gttgggttgg gttgggttgg gctgggcttg ctatggatcg
     3121 tggatagcac tttgggcttt aggaacttta ggggttgttt ttgtaaatgt tttgagtcta
     3181 agtttatctt ttatttttac tagaaaaaat acccatgcgc tgcaacgggg gaaagctatt
     3241 ttaatcttat tattgttcat tgtgagaatt cgcctgaata tatatttttc tcaaaaatta
     3301 tgtcaaatta gcatatgggt ttttttaaag atatttctta tacaaatccc tctgtattta
     3361 caaaagcaaa cgaacttaaa acccgactca aatacagata tgcatttcca aaagcgaata
     3421 aacttaaaaa ccaattcata caaaaatgac gtatcaaagt accgacaaaa acatcctcaa
     3481 tttttataat agtagaaaag agtaaatttc actttgggcc accttttatt accgatattt
     3541 tactttatac caccttttaa ctgatgtttt cacttttgac caggtaatct tacctttgtt
     3601 ttattttgga ctatcccgac tctcttctca agcatatgaa tgacctcgag tatgctagct
     3661 ccgcaagagg cggccgctgt aggcacacaa gtaggggagg tgtagttcaa ctcatgatct
     3721 atcatatccc tactagttca taaattgatc atatctttca catccaacat ttatgatgtc
     3781 acatgtgctt caaagatgaa tatttgtttt gggaccaatg tttatatctt cgcggtgcca
     3841 agccaaacat actcaaacat ggtggctacg gccaccgtcc tcctcttgag gaggttggat
     3901 cttaatttgc attttatttg ggtattctac aagtcaacta taggctaaat atgatcatac
     3961 actcatcatt tggtgttttt ttcgttcgtg caaatatgag tgcataagat ctctatacac
     4021 ccttttagtg tgattctatc aaatgccttc ttatctaaaa gacatgtttt cacctcttgc
     4081 aagtgttcct cttggcttaa actcaagtag aattggattt cattgcgaca atgtcctttt
     4141 gtgtgagggc tctacagctt ggaacttgtg aaggtcagac aagtctaacc acctaagtcc
     4201 cttttcctat actggtctca aatgcttgtg gacaccccgt gcacatgagc ttgtaccatg
     4261 cgcatggggc cccatgcaca tggggttgag gttccaggcg gggtatgagt aggcgcgcgg
     4321 ggtggttagg gttccccact ccacatccaa cccccacgcg cccagacgac tcactccgct
     4381 gccaccgttg cctccttcat ctccgacgag atcacgtacc cacaggcctc tccctcggca
     4441 tcctcttgct ggaatgggga gctcctccgg tacgtgatcc cgcgcctctt attcattcga
     4501 tgaaactcct cctttttcct ccatatattt tagggtttcg tccactgctc gggtagattc
     4561 acataatcta ggactaaatt ggaatagaaa ctgctaggaa ccatctggat gggtatagcc
     4621 gcattcactc ccgtggattt atccttggag tggccatggc tggagcaccc catgtgcacg
     4681 ggcttgtacc atgcacatgg ggctcacctc ccgtgcacac atggtatcca gaggagttcc
     4741 acatgtttct tttttagttt tgttcttctg cacataacca aacacttgct cttatcttct
     4801 ctgtctatct atctctctca gatatgtccg ctagcaccgt ggaggcttct catagcaaga
     4861 agaccaaccc ctccgagagc gcctccaact tcaccatctg ttgtcgtgag ccagtggatt
     4921 cttctgcagt ccatcagtca gacggtgtac gcagggcaac caacaagaag gtcgatgcag
     4981 cttctcatgg tgttgagatt cagcctggat ctgaggacgt ggatagctag gctaccgact
     5041 atgataaccc actagcagga tggcgctttg atgatgacca ggatgatctc acgagtggcc
     5101 ctctgcgtgt ctaggtcacg ttagcctctc ctgttccctt tttggcgtct tgatgccaaa
     5161 gggggagaag tgttctatat aggagcattt gtatttggga gatttcaagc cttgttcttc
     5221 attgttggct tttatgtggc ttgaaatact tttattgatg tgagtcactt ttttgtgatg
     5281 atgctatctt tatgacttgg tatgctatgc ttattgattc catgtgatca tatcatgtca
     5341 tctattgctc atagtctttg atatattgct ctcacaccac acctcttgac tgtgatctct
     5401 agcacaagtg gtcgccggtt ccagcacgag tggtcaccgg ttccagcatc caccgcgtcc
     5461 ggttctagca atgttgcaac gccgtcgaag cacgaccgca cgccgggtcc agcacgaccg
     5521 gttccagcat caccgtgtgc acgttccatt aatgctacaa ctccgtcgaa ccacggccga
     5581 gcgccggttc ccgcacgaat gccgtgcccg ttctgccggc ggccatccgt cgtaacatct
     5641 ccgtggcctc gcgtcgcagc gccggtgggc tctagcatcg cagcaccggt ggctgcccgt
     5701 cgcagcggtg ccggtggacg ctcgtcacag ctccacccct tgtagcagcg cctgcgacaa
     5761 gccggcaacc tgaaaacgtg cggcgtctgg ggattttgca gcagcgcgca gcccctgtag
     5821 cgcggcggtc aggctcgaga tagcggggga ttcaggcttg cgcgcggcgg tggtcaagga
     5881 ctcggcggca gtgaggattc agcatgcacg gcatgctccc agctgcggtg cgcaagatga
     5941 gaggaaggaa gcgacgagat agaggagatg caggaggaag aaacgagtgg ttcgtctggg
     6001 gataaggttg ttgcgaaagc ggtggaagca gggcccacag aaggacacgc ggccgcgcaa
     6061 aagtggagga ccggctacgc gtgggaaagg caggaaggat gctgagcgca cgatctcatt
     6121 gatcgagcgg tgcaagcgcg accggcgaga gtcaaggccg gtcggctgat acagtacccg
     6181 cgagaggcga aatgaaaagc gtcgagtccc ctcgttccgc cggcgaccag accgagggtt
     6241 ccctccctct ccgtccgctg ctccagcagc gcgaaccccg gatctgtgtc tccggtgtgt
     6301 acacagtagg gtaggtgctc ggatggtggc gcggtggggc caaacgcggc gtaaaaaaat
     6361 cagggggtat gtacatatat cttactggat gcagaacaaa aacagatgca tacaaagacg
     6421 agtggacgaa aaatccaagg tcatggtagc tttcactttc aggcagacga gttagtgggc
     6481 gacatccacc ggattcagat aaccacaaac actatagaaa tagaataaag tccccaaaag
     6541 tccaaacaat gaattgatca gctcatcatc gtcgcaagga gctgtagccc aagaggccaa
     6601 gaccatcacg agctggcggt tcattcccgt tccaggagcg ttgaccagct ccacatcgtt
     6661 cctcacagca gcctttttca aggttagtac tagtgctttg ctctctgcct ccctcccaga
     6721 cgtctcctac tgcttgcttt aattcgtaac ctgtggttaa attcatgctt ctttgctctt
     6781 caagatcgga gctcgtttca ggcccagtct gcgaacccac ccaatttccc ccttctgtgg
     6841 aactcaaccg tttgatacac atggaaccat agcggagatc catatacacg tttaattttg
     6901 tattgtattg tgtttccagg catggataat atatttcgca aattcggatg gaaagctggt
     6961 agacggcaac aaggagaaag gcagtttgat tcgccatgag tctcgattcc ttatacaggc
     7021 ataaaaattt ctctgatgtg acgagtgaaa ttgctatatt tatttttgcc taccaggaga
     7081 gggaatagca cgggcagggc gtcgccaagg cctctttcgt accatttatt aggagaaatt
     7141 acaaatggtt tctcggagaa ccgaaaactt ggtactggtg catatggaag tgtttacaag
     7201 gtaagatttc agccacaatc tcatgaagtt agacatgggg tggtacttgt ggatgcacac
     7261 cacacattat ttggtcaaca acttgctatt caccagccac tacaccatta ttcagcggtt
     7321 aacgagagta ccatgcatgt acaaccatta aatgcataca gaagctaact cgtgtatata
     7381 aatacaggca cattcaagtt cacaggccac acatatatct tttgatcttt tttttttttt
     7441 ttttgcgaga aaatttccaa tctattaata ttcaatcatg gcagtacaac gaatatcaga
     7501 aataatggaa attacatcca gatccgtaga ccacctagcg acgactacca gcactgaaac
     7561 gagccgaagg cgcgccgccg tcatcgcccc tccatcgccg gagtcgggca caacttgttg
     7621 tagtagacag tcgggaagtc gtcgtgctaa ggccccgtag gaccagcgca ccagtactac
     7681 aaccgtcgca gatgaagaat aacgtagatc agaaagatcc aatccgaaaa cacacgaacg
     7741 tagacgaaca acgacgagat ccgagcaaat ccaccaaaga tagatccgcc ggagacaaaa
     7801 ctccacacgc ccaccaatga tgctaggggc accgccggaa cgggggctag gcggggagac
     7861 ctttattcca tcttcaggga gccgccgccg tctcgtcttc ctgatcagga cacaaaccct
     7921 agcaaaactg aaagaaacaa ctaaaaacgg agccctcccg ccggccattg ccgagatcca
     7981 ccacgccccc atggcccttt tgatctactg atcttgttaa tgactagcat gcataccccc
     8041 gcaaaaaaaa aaaatgacta gcttgcatac ttggtcttgc gccgctgtgc aattttacgc
     8101 catataacaa caatacaccc tcggtcccat aacatggtgc acatggtttt cgcgaaagtc
     8161 aatcttcaca aaatttgacc aagttaattg agaaaattgt ctatgtctac actaccaaat
     8221 atataccata tgaaagtata tttcatagtg aatctaatga tattgatttg gtattctaga
     8281 cgttgataaa ttttcctata aacttagtca atgtttacac agtttgactt tgaaaataat
     8341 ctaatacaca ctattttttg ggggacggag ggattataag ggcatttcta accaatccct
     8401 tataacatgt tagcctctaa ccgggaccta aaaaatctat tcggccgccg caaatctcac
     8461 tatttttact ccaccgctcg gcggccgagt aaaaatctct ccctcgcctc tgtcgcctct
     8521 tctcccctcg ccctgccgcc ggcgcgtccc ccgcccttcc ctgccgcccc ctctcctccc
     8581 aatggccgga cacggtggcc ggaggtcccc tccgccacgc cggcgggtcg tcgtccgccg
     8641 gtggatcctc gatgcgcccg ccctcacccg gttcgccgat ttcccggagc tccctccgct
     8701 gcggccgtcg cagaggacct acctcggcgt ccgacaacgc tcgtggggga catgggtggt
     8761 ggagatcacc gacctggaga cccaccaatg gaagtggatt gggtcgttcc acacggcaga
     8821 tctcgcggcc atggaatacg actggtggta agtccggtac cacggctccg tcgcccggct
     8881 gaactttcca ttcgggacgg cgccggtcca ccttcttccg ctagagtcgt gtgtgctgag
     8941 cgcggcgata gcgtaggagg accgagagcc gagggagtgc ctcgaggtgg aggccgccgc
     9001 tgaggcgtac atggatgacc tccgccgcta gcacccggag ctcgtggagg cggagcggct
     9061 gatattcgcc gacaatggcg gggaggtcat cgtcatctcc gacgatgagg tggaaggagg
     9121 cgaggagggc agcaagaata ctgcgctatt ttttagggga tcaattagaa atgccctaac
     9181 aatagagaat acgggtcaac atatattcta taatgagtat taactcattc tgctctgtac
     9241 ttgaaactta taccgctaca aacaggaaac taaaccattc ttgtgaaata ttgaataggg
     9301 ggagcataaa gacggggaaa agattgctgt gaagatgctt catgatactc taggacttga
     9361 caatgagcaa tttgaaaagg agtattttaa ccttgcagat ctacagcaca aaaatattgt
     9421 ccgattagtt ggctattgcc atgaaactcg gcgagaatgt gtgccttaca acggaagaat
     9481 ggttttcgct gacataacaa agagagcgct ttgctttgag tatatgcgta atggaggtct
     9541 tgacaaatgt ctttcaggta taatctatgg gtaatctact tgtattagga ggtagaaatg
     9601 caaggaatag ttattaatat acgtttcgcg gatattttct atgtttatgc agatgaaact
     9661 agtggacatg attggtgcac acgctattca atcattaagg ggatttgcga gggtctgaaa
     9721 tacctgcacg aggaattgga atctcctatg tatcatttgg acttaaaacc agccaatata
     9781 ttactcgatg agaaaatggt gccgaaaatc gcagatttcg gcttgtcaag gttcttcaga
     9841 ggagagcaat cgcaaatcac aaaaagtgct ataggaacac agtaagctta cactatcatg
     9901 tgaactgaaa ttttctttag caggaaattc tgtttgcact aaatatacgg ccttttttta
     9961 aacatatatc ctgtatcatg cagtggatac gtaccgcccg aatacataga tgcatctgta
    10021 ctctcaatta agtttgacat attcagcttg ggtgttgtaa tcataaagat aatgactgga
    10081 ccaacaggct acttcagaag tgctgaaatg tctgcaaaac aattcataga acttgtaagc
    10141 aaaattgttg cctttacaga tatcaatagt aaatatttcc gaattaaact gccatcttta
    10201 ctgatatact atggtttttt atgttccgta ctggtaggta ctggcaaact ggaggaagag
    10261 gctacctgca acatcagtgt acctgctgga gtcatactcg gaacaagtaa agaggtgcac
    10321 cgcaatagct gtaagttgtg tggaggctga tcgatccaaa aggccgtcta taggggaaat
    10381 tgtgaataag ctgaatgaga cagagacaat gattcaaaat gtggcaagga aacatacacg
    10441 atcgtcggtg gaccaggtgt gatcactaat ggcacaagtt atttacttgt gttttctatt
    10501 ttcactataa aataaaataa ataaagtgaa attagcacgc atgcatgtat gtatacagag
    10561 agtagtgcta atatacccca gtactgaata aaccatttaa tgtttatagc gatgcacata
    10621 gcaatatttc ataattttgc caccacatta gtaagtccag cataaccagt tcgaaacgaa
    10681 catgtcttcc tatgttagat gaacatgtct tcctatgtta gattgttagt ttctttgtat
    10741 aagctagcac atgttattgt ttcttataag ggacaagacg gcctcctttc ttccctttgc
    10801 agtgtgctat aacttaccaa ctttcaattc attgttcaaa acatagaaat cagagattat
    10861 tacctgagaa gacagcagta tccttttcca attagctcta acatactatt ttatactagg
    10921 tatcagaacc agttacattt ataggaggag ctgttggggt gttagatacc cttggcggtg
    10981 ttagcttaaa taataatcat ggcagtggtt tcttgggcgg aggaacaaat cgaggaagta
    11041 aagtcagtat tttggcattt gaagtcgcaa atacaatagc taaagcttcc gatttgtgga
    11101 gatcatgctc tgacgagagc ataaaagaac tgaaggaaga aattttgcat tcagatggtg
    11161 tgcgaatatt aatttcctcg aattccagtg agctcctgca tattgctgct atcggcaaga
    11221 ggtatggtta gtttcaaagt aagttttttt tattcatctt actgtaaacg ttgttgatgt
    11281 ggatggaacc caacagggaa gaactcgcca tcctttcaag agaagtaatt cgatttggtg
    11341 acctgtttat agaccccata tggcataact tgagacgcta ttttgaaaag tatgcttctg
    11401 agaattatgc atttcatttc gacatgtcat tatttaccgt cttatcagca cctcttgttc
    11461 tgaacactat gggttactct ggtgtgcagg tcaacgaaag attccatgcc ccaggatcat
    11521 tcaaaagagc atatcggaac tactgtccag cagttgatta gtttggctca aaacacttct
    11581 gtatgttcta cttaatttcc acattcctat atttcagaag gtgcttgtac ttgataataa
    11641 tactaacgtt tatggagtca agaagtggca tcatcattac aactgtactt cttgaaatta
    11701 ttgaacggct aaacatcttt aatgaaatca agaatctttc agcaggttaa taaccaagaa
    11761 tcaggtctct tgtatatcag ccagctagaa ctacagtctg agggtggatc aggctatttt
    11821 tttgcattca tactctggct tgaagcttat gctgatgagc tagttctata actgcagaag
    11881 ctttaccatg aattgcacgc tctggataga ttggcgcagg aatttcaaag gaaatttcag
    11941 gaagaggagt ctgtaccagc aactagacga ggtacaacca gactgatgac attgttctta
    12001 agcaacttca gtagatgcta aaactgaatg catattgctt ttccagtatc tcacccggaa
    12061 ataacgactg tactacataa attaagactt tagtgaagag atcttaatac agttttttta
    12121 ctacgagcac tgtagttttc catcaaaagg ggaaaatgat gcttttgttg gttgtgatgc
    12181 atttttttac tctgaacgtt ctctgctgat attttatctt acctattgcc aatccaaagc
    12241 tccaagtgaa caaaattttg acagctgctg tgattattga taaatagtgg taaaccaagc
    12301 atcacagcta aggttcttac atggagaacc agggcctaga ggaaaatttc acatttttag
    12361 ataatacaag aaatgccaat ttatcttcct ggcaatgcca ttggaggcat tctgataaat
    12421 atttaaatat ctttgtcaca gtatactttt atacagcctt tttttataga tgatatgata
    12481 ttaatttaag acaccatgta ctgcaaattt gctactatta gagatattta ttgtattcaa
    12541 tttttgctct ttgcagagag tgttatgatt ttgcacagtg aactaaagcg ccaatggaag
    12601 cttgtgaaaa atttgaagaa gaaatccttg tggtccagac ctttggagga tgtaattatc
    12661 ttcatccaca tttgcttcat tacttcggcc ttctgtaatt ctgtctgttg ggttatgcct
    12721 ggcagcgttt tgttgagata gtttttgatg aatgcgctac tggcatattt gctttctttt
    12781 gatggatgct gacattgctt tgtgttatcc tctatccaga ttgtggaaaa gcttgttgat
    12841 atcgtcagtt ttctagataa acaaatccgg gatgcatttg gtgaagccgg taattgatta
    12901 ttgattggct gctttattgt tcgcagaaaa tgtatccgag tacctgacaa tatcctgaca
    12961 cctctctgaa taaacagttc ctgtaggtac tgacttcatc gagcaagttc agaacaaaag
    13021 gctaggttcc tgtggtctgg cactacatta cgctaacatt atcaatcaaa ttgaaaatat
    13081 agtaagttaa tttgacgacc catgtcatgt ttattgtgtt tgcttaatgt agccaatttc
    13141 catgtgacct cttatttatc tatgaaacta tttctgttat gtcgatgaac cgtgcattta
    13201 tttatttccc tttcctttcg gtgaacagaa gtgttcaata gtgatctgta atgaatattt
    13261 tatggtcacc tgcataaata tcagtctatc tcctagctgc aaaataccaa tccgaaagta
    13321 atttttgaaa taaagaagtt acaacgtcat agtgcatata ttgtatcacc gaagtctaca
    13381 tgcttcagtc tacgtcgttg cttaagaaat aagaaaaagg catagagttc tcaatctttt
    13441 gtgtacatat gcaggtttct cggccactct ctcttcctcc tagtgctagg gacaacttgt
    13501 accatggact accagtaaca gtgaagtcag ctctgcggtc aaggttgcaa tcagttgata
    13561 ctgaagagga ggtttgtctt acacaactcg gcagaatatt tatttaatta tgcacatgga
    13621 atatatagta gaaacttata actggtggtt gagcttagcc accagaatac tgttttatgt
    13681 ctgtccagac atgattcagt tctaagcact gaaagtttag ttgtccatgt gggcagtgta
    13741 accacatgat atccaaagga gcaaatacat gggatacata ttgtagaatg tgataggcat
    13801 tgtgcatata ttttcttgac aagagccagg acttgatttt gttgctatta ttggtacatc
    13861 aaagcgtatg actgaattgc gatccttttt ttattctctg cagcatactg tatctcaaat
    13921 caaagctgaa atgcagaaaa cccttcgctg gattctgcca atagcagaaa atacaaggag
    13981 gtacttcaaa tcattctgat ttctgagcat agttgttttt ctccttgttt acaaaattca
    14041 ctgtttccat acttgtacaa gagcacacca agtatttgga tgggtcggcg aatgggcaca
    14101 ctttgggtta gtctcctcaa atatcatgat ttcctcatct tatccccttt gaatttctat
    14161 cgaatatatc gtatacactt gtttgcaaca taaccttttt tttttttttt gcgaatttgt
    14221 ttgcaacata acctgacgta gagattttac aggagtgaca tggatgagaa gtcaggttcc
    14281 cggcacagcg tgatcggggt ccagacgctt catcatgccg acaaggcgaa gacggaggag
    14341 cacatcctcg agcttgtggt gttgctccat catctcgtgg tccaggtgaa aagccgaggc
    14401 tacgagcaca acaattccac aaagcaggac cggtctcggt cctgcaaggt atcatcggaa
    14461 ccgcaagccg atgccaagca ctacacatct ccgacgaaca actgtgagat ctgcccgagc
    14521 ccgctgtcgg actccgagcg ccggacgctg gaccacctga gcttcaagag ctgcgagcct
    14581 cagcctggca gggtggacaa ggcgcatagg agcggggact cgtgccggag ccatggcagc
    14641 tcgccggcga gggagttcag tcgtagcttg acctcgggcc gtgagctggt gaaggatctg
    14701 gatgtaatag atggtctagc agactgacta tcgtttagtt aagagtgaag acatttggtt
    14761 gcacagcaga gtaggtattc tttggccttt gccgccgcct tcttctatct cgtcgatcag
    14821 atcgtatata tcttgtatgt gtgttttcga ccgttacata tgtagattat ataaatgaca
    14881 gtgttacagc tgggttgtga tttgaatgga aatatagcaa gatcacaagt gatgctccat
    14941 tgcctccatt gtgtcactct gtatactgcg agcatccaca gtgtggtatt ttgctgcctc
    15001 tatccttgcc tctaagctat ttggagctgc ctctatacat tgcatggatg agcctctaag
    15061 caaaaatgct tggcgtcgcc tctaaggtaa gaggcagcct ctaagccttt tcttggacta
    15121 aaataatggc atggccctat gggtgtgtac acataaaagc tggctggtga agttttgttg
    15181 ataaaataat attactttct actgtggtgg gatccatgtt agaggctgta tcaaagattg
    15241 aacattgcag gttaggacgc ttagcttaga ggcagcatgg tgggacccag catagaggca
    15301 gcagcgcctc caaacactgc tgatgcttta tgatacctga attttcagtg tgtcggcagc
    15361 ttgcctttac tagagtttat tgtgactttc tgaacacttg atcagtagaa cctcccaata
    15421 aaactgtttg gatctttctc atcgaaggcc aaataaaacc taggactccg cttgaaatgg
    15481 tttgtagaac tagcgcggcc accggtactc aagtcaattt tgaactagaa attagagata
    15541 attgttccag agggcctgca tgcttgtgaa gtcctcctct actcccaatc tcccacgcat
    15601 acgcatgcta ccctttttct tcttcttaaa aaaagtgtgt tcgcaaaata ccatcgccat
    15661 gattcggccg gggtggtcca gagcttatct actgctaact gagtactact actactgata
    15721 gtgtcttctc ttctcttcac caggtcatcc atcttatcag gatatcacat atcattatat
    15781 caacaataat gcaagctatc tactccctcc gttcctaaat atacgtcttt ctagagattc
    15841 cactacggac tacatacgga gcaaaatgag tgaatctaca ctctaaagta cgtctatata
    15901 catccgtatg tagtctggtt tgaaatcttt aaaaagactt atatttaaga acggcgggag
    15961 taaaaaataa taataatgca agcaagcact tttcccatac atatccgtac cctattcatt
    16021 tcacggcgac gtccacgtcc atatgtccgc cgctcccctc atccccagct ctgccaccca
    16081 cgtgtacgcg gcgccgagag agatgagacc ccctgccgta cgtcgccgtc gacgtcgcca
    16141 tggcgcgcgt ggcacggtcg gcgtcggcgt ctccgacgac ccacccaccc gccgaattga
    16201 taacgccgtc catcacggct cggtctgccg ccagctgcat gcatggccga ctggcttctt
    16261 gtcggcgctg cccgcctaga catgccatgt gcaagcctga gataggcagc gcataacacg
    16321 tacgaccaca ggagggcatc tctgaacacg taccatacga gtagcttcag cttcggagat
    16381 actagcatgc atcttgaacc atctagaaca ggaagaaaaa caggctaatt aattcttggt
    16441 ggtgattggt gacaagacaa acgcggtgtt tgtgtagaaa aatcagaggt actgtgtcca
    16501 tctggccttg acggagaacg tgatcagata cgaagaaaca tgagtgcaga aaaatcagag
    16561 gtcacggcag atcattgcgt gcagatgcat acgtagctat cactttcagg aagacagatt
    16621 actgggcgac gtgcaccgga ttcagataac cacaaacact atagaataac aaagtcccca
    16681 aaagtccgaa cgacgaaaga tatgtgacca cacaatcgat tagcccatca ccgttgcgag
    16741 gagctatggc ttaccttgga gaacaaagga aaaatctatg atctccattt ggggcgtcca
    16801 ggaaaaatgt catgaattct tgtaaaacaa gtcaacgata tgaaaataga gattcgaaat
    16861 tcgaacgttc cagataaaca caaacgctag aataaacaac tagtccacac cgtgaaagaa
    16921 tatcatcacc atctgtcagc atcgcgagac ctagctcggc aagaacaata acggaaagcc
    16981 tatcgttctt gttgggaatc caaacacaaa gtctcatgta ctattttttc taaaacaagt
    17041 tggccatatt gaaccatagg gtgacaccta tgtatatgat ggcaccgaga tttcaaaatt
    17101 caaattcgac atgtcagcac gccaagaaaa caaacagtac tatccacagc tccatctgcc
    17161 atcttgacca gaagcttttt attttccgaa gcacgaggaa gactgagtgg gtttaaacga
    17221 cgagggacac gaagtgatcc gtttccttga caggatatat tggcgggtaa actaagtcgc
    17281 tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    17341 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    17401 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    17461 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    17521 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    17581 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    17641 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    17701 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    17761 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    17821 gcgctctgct gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac
    17881 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    17941 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    18001 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    18061 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgtgtaacat tggtctagtg
    18121 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
    18181 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
    18241 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
    18301 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
    18361 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
    18421 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
    18481 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
    18541 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
    18601 attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat
    18661 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
    18721 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
    18781 acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga
    18841 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
    18901 gccttgagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
    18961 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
    19021 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
    19081 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa
    19141 tccccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc
    19201 tggatgatgg ggcgattcag gcgatcccca tccaacagcc cgccgtcgag cgggct
//
