LOCUS       sequence-382716-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian
            cells with localization in the mitochondria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Martin A, Rivera-Fuentes P
  TITLE     A general strategy to develop fluorogenic polymethine dyes for 
            bioimaging.
  JOURNAL   Nat Chem. 2024 Jan;16(1):28-35. doi: 10.1038/s41557-023-01367-y. 
            Epub 2023 Nov 27.
  PUBMED    38012391
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-023-01367-y"; journalName: "Nat Chem"; date: 
            "2024-01"; volume: "16"; issue: "1"; pages: "28-35"
COMMENT     Sequence Label: TOM20-SNAPf-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             472..1017
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gtgggtcgga acagcgccat cgccgccggt gtatgcgggg cccttttcat tgggtactgc
       61 atctacttcg accgcaaaag acgaagtgac cccaacttca agaacaggct tcgagaacga
      121 agaaagaaac agaagcttgc caaggagaga gctgggcttt ccaagttacc tgaccttaaa
      181 gatgctgaag ctgttcagaa gttcttcctt gaagaaatac agcttggtga agagttacta
      241 gctcaaggtg aatatgagaa gggcgtagac catctgacaa atgcaattgc tgtgtgtgga
      301 cagccacagc agttactgca ggtcttacag caaactcttc caccaccagt gttccagatg
      361 cttctgacta agctcccaac aattagtcag agaattgtaa gtgctcagag cttggctgaa
      421 gatgatgtgg aagcgccagc atttaaatct gtacagaccg gtgaattcac catggacaaa
      481 gactgcgaaa tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg
      541 tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc
      601 gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc
      661 gcctggctca acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc
      721 ctgcaccacc cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg
      781 aaagtggtga agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat
      841 cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc
      901 ccctgccacc gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc
      961 gtgaaagagt ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggt
//
