LOCUS       sequence-382718-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian
            cells with localization in actin filaments..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Martin A, Rivera-Fuentes P
  TITLE     A general strategy to develop fluorogenic polymethine dyes for 
            bioimaging.
  JOURNAL   Nat Chem. 2024 Jan;16(1):28-35. doi: 10.1038/s41557-023-01367-y. 
            Epub 2023 Nov 27.
  PUBMED    38012391
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-023-01367-y"; journalName: "Nat Chem"; date: 
            "2024-01"; volume: "16"; issue: "1"; pages: "28-35"
COMMENT     Sequence Label: LifeAct-SNAPf-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..48
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="GVADLIKKFESISKEE"
     CDS             127..672
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 ggcgtggccg acctgatcaa gaagttcgag agcatcagca aggaggaggg ggatccaccg
       61 gtcgccaccg gatccgaaat cggtactgcg ccagcattta aatctgtaca gaccggtgaa
      121 ttcaccatgg acaaagactg cgaaatgaag cgcaccaccc tggatagccc tctgggcaag
      181 ctggaactgt ctgggtgcga acagggcctg caccgtatca tcttcctggg caaaggaaca
      241 tctgccgccg acgccgtgga agtgcctgcc ccagccgccg tgctgggcgg accagagcca
      301 ctgatgcagg ccaccgcctg gctcaacgcc tactttcacc agcctgaggc catcgaggag
      361 ttccctgtgc cagccctgca ccacccagtg ttccagcagg agagctttac ccgccaggtg
      421 ctgtggaaac tgctgaaagt ggtgaagttc ggagaggtca tcagctacag ccacctggcc
      481 gccctggccg gcaatcccgc cgccaccgcc gccgtgaaaa ccgccctgag cggaaatccc
      541 gtgcccattc tgatcccctg ccaccgggtg gtgcagggcg acctggacgt ggggggctac
      601 gagggcgggc tcgccgtgaa agagtggctg ctggcccacg agggccacag actgggcaag
      661 cctgggctgg gt
//
