LOCUS       sequence-382720-         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian
            cells with localization in the endoplasmic reticulum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Martin A, Rivera-Fuentes P
  TITLE     A general strategy to develop fluorogenic polymethine dyes for 
            bioimaging.
  JOURNAL   Nat Chem. 2024 Jan;16(1):28-35. doi: 10.1038/s41557-023-01367-y. 
            Epub 2023 Nov 27.
  PUBMED    38012391
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-023-01367-y"; journalName: "Nat Chem"; date: 
            "2024-01"; volume: "16"; issue: "1"; pages: "28-35"
COMMENT     Sequence Label: SNAPf-mTurquoise2-KDEL
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..543
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
       61 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
      121 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
      181 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
      241 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
      301 ctgctgaaag tggtgaagtt cggagaggtc atcagctaca gccacctggc cgccctggcc
      361 ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt
      421 ctgatcccct gccaccgggt ggtgcagggc gacctggacg tggggggcta cgagggcggg
      481 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
      541 ggt
//
