LOCUS       sequence-38274-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of nucleus-targeting red fluorescent 
            genetically encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-NLS-R-GECO
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..28
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             32..52
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             68..124
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 catggaccca aaaaagaagc ggaaggtcga tcctaagaag aaacgcaagg tgatggtcga
       61 ctcatcacgt cgtaagtgga ataaggcagg tcacgcagtc agagctatag gtcggctgag
      121 ctcacccgtg gtttccgagc ggatgtaccc cgaggacggc gccctgaaga gcgagatcaa
      181 gaaggggctg aggctgaagg acggcggcca ctacgccgcc gaggtcaaga ccacctacaa
      241 ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt tggacatcgt
      301 gtcccacaac gaggactaca ccatcgtgga acagtgcgaa cgcgccgagg gccgccactc
      361 caccggcggc atggacgagc tgtacaaggg aggtacaggc gggagtctgg tgagcaaggg
      421 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      481 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc
      541 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      601 cctgtcccct cagttcatgt acggctccaa ggcctacatt aagcacccag ccgacatccc
      661 cgactacttc aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga
      721 ggacggcggc attattcacg ttaaccagga ctcctccctg caggacggcg tattcatcta
      781 caaggtgaag ctgcgcggca ccaacttccc c
//
